Poiseuille flow in a nanochannel – use of different thermostats

Poiseuille flow of a liquid in a nano-channel is simulated by molecular dynamics by embedding the fluid particles in a uniform force field. The channel is periodic in y and z directions and along x direction it is bounded by atomic walls. The imposition of the body force generates heat in the system leading to shear heating and a non-uniform temperature rise across the channel. In this nonequilibrium system, one can attempt to control temperature in different ways: velocity rescaling, thermostats or wall-fluid coupling. We evaluate and compare different methods critically by analyzing the fluctuations and time averaged quantities from various simulations. When particles will be inserted into the flow, it is expected that the dynamics will depend on the thermostat chosen. First observations show little influence of the thermostats on single tracer particles – this needs further study

Meloxicam decreases the migration and invasion of CF41.Mg canine mammary carcinoma cells

Indexación: Web of Science; Scopus.Cyclooxygenase (COX)-2 expression is positively correlated with malignant features in canine mammary carcinomas. Non-steroidal anti-inflammatory drugs (NSAIDs) inhibit COX activity and may therefore possess anticancer effects. Meloxicam is an NSAID that is widely used in human and veterinary medicine. High concentrations of meloxicam have been reported to be antitumorigenic in vitro; however, the effect of meloxicam at concentrations that are equivalent to those that can be obtained in vivo remains unknown. In the current study, the in vitro effects of low-dose meloxicam (0.25 μg/ml) on CF41.Mg canine mammary carcinoma cells were evaluated. The effects on cell proliferation, apoptosis, cell migration and invasion, in addition to the expression of different molecules associated with tumor invasiveness were analyzed. No effect on cell viability and apoptosis were observed. However, cell migration and invasion were significantly reduced following treatment with meloxicam. MMP-2 expression and activity were similarly reduced, explaining the impaired cell invasion. In addition, β-catenin expression was downregulated, while its phosphorylation increased. These results indicate that 0.25 μg/ml meloxicam reduces cell migration and invasion, in part through modulating MMP-2 and β-catenin expression. Additional studies are required to elucidate the mechanism associated with the anti-invasive effect of meloxicam on CF41.Mg cells. The results of the present study suggest that meloxicam has a potential adjunctive therapeutic application, which could be useful in controlling the invasion and metastasis of canine mammary carcinomas.https://www.spandidos-publications.com/10.3892/ol.2017.640