How a grafting anchor influences cellular uptake and in vivo fate of dendronized iron oxide nanoparticles

peer reviewe

Evaluation of the active targeting of melanin granules after intravenous injection of dendronized nanoparticles

peer reviewe

Chemotherapeutic errors in hospitalised cancer patients: attributable damage and extra costs

<p>Abstract</p> <p>Background</p> <p>In spite of increasing efforts to enhance patient safety, medication errors in hospitalised patients are still relatively common, but with potentially severe consequences. This study aimed to assess antineoplastic medication errors in both affected patients and intercepted cases in terms of frequency, severity for patients, and costs.</p> <p>Methods</p> <p>A 1-year prospective study was conducted in order to identify the medication errors that occurred during chemotherapy treatment of cancer patients at a French university hospital. The severity and potential consequences of intercepted errors were independently assessed by two physicians. A cost analysis was performed using a simulation of potential hospital stays, with estimations based on the costs of diagnosis-related groups.</p> <p>Results</p> <p>Among the 6, 607 antineoplastic prescriptions, 341 (5.2%) contained at least one error, corresponding to a total of 449 medication errors. However, most errors (n = 436) were intercepted before medication was administered to the patients. Prescription errors represented 91% of errors, followed by pharmaceutical (8%) and administration errors (1%). According to an independent estimation, 13.4% of avoided errors would have resulted in temporary injury and 2.6% in permanent damage, while 2.6% would have compromised the vital prognosis of the patient, with four to eight deaths thus being avoided. Overall, 13 medication errors reached the patient without causing damage, although two patients required enhanced monitoring. If the intercepted errors had not been discovered, they would have resulted in 216 additional days of hospitalisation and cost an estimated annual total of 92, 907€, comprising 69, 248€ (74%) in hospital stays and 23, 658€ (26%) in additional drugs.</p> <p>Conclusion</p> <p>Our findings point to the very small number of chemotherapy errors that actually reach patients, although problems in the chemotherapy ordering process are frequent, with the potential for being dangerous and costly.</p

Fast switch from extensional exhumation to thrusting of the Ronda Peridotites (South Spain)

International audienceThe Alboran Domain, situated at the western end of the Mediterranean subductionsystem, is characterized by the Ronda Peridotites, one of the world largest exposures of subcontinental mantle. Using U-Pb (LA-ICP-MS) and Ar-Ar dating, we precisely dated twotectonic events associated with the Tertiary exhumation of the Ronda Peridotites. First,shearing along the Crust-Mantle Extensional Shear Zone caused, at ca. 22.5 Ma, mantleexhumation, local partial melting in the deep crust and coeval cooling in the upper crust.Second, the Ronda Peridotites Thrust triggered the final crustal emplacement of theperidotites onto the continental crust at ca. 21 Ma, as testified by granitic intrusions in thethrust hanging-wall. The tectonic evolution of the western Alboran Domain is thereforecharacterized by a fast switch from a continental lithosphere extension in a backarc setting,with sub-continental mantle exhumation, to a rift inversion by thrusting driven by shorteningof the subduction upper plat

Altered heterochromatin organization after perinatal exposure to zidovudine.

International audienceBACKGROUND: Zidovudine (3'-azido-3'-deoxythymidine, AZT), administered to pregnant women alone or in combination with other antiretroviral drugs, greatly reduces the mother-to-child transmission of HIV-1. The potential genotoxicity of these molecules is underestimated and wide-ranging evaluation of its biological and clinical consequences is required. METHODS: We investigated the nuclear organization of constitutive heterochromatin, a major domain participating in epigenetic regulation, in uninfected infants born to HIV-1-infected mothers treated with zidovudine and/or other nucleoside reverse transcriptase inhibitors (NRTIs) during pregnancy. We studied the organization of chromosome 1 heterochromatin (1q12) in peripheral leukocytes of 25 HIV-1-uninfected children (newborn to 9 years old): children born to HIV-1-infected mothers exposed to zidovudine and/or other NRTIs (n=15), children born to HIV-1-infected mothers not exposed to any NRTIs (n=6) and children born to HIV-1-uninfected mothers (n=4). RESULTS: Results differed significantly between NRTI-exposed and -unexposed children. By contrast, there was no difference between NRTI-unexposed children born to HIV-1-infected mothers and children born to HIV-uninfected mothers. The anomaly persisted in lymphocytes cultured for 48 h. There was no evidence of abnormal DNA methylation, a major feature of constitutive heterochromatin and associated with the loss of its structure. In a complementary sample of children, analysis of chromosome 11 and 16 heterochromatin suggests that the defect affects most of the other heterochromatic sites of the human genome. The heterochromatin defect persists long after the end of the exposure and appears in leukocytes of both myeloid and lymphoid lineages, suggesting that haematopoietic stem cells are affected

Peptide screen identifies a new NADPH oxidase inhibitor: impact on cell migration and invasion

International audienceno abstrac

Clinical value of serial quantitative analysis of cytomegalovirus DNA in blood and saliva over the first 24 months of life congenital infection: the French Cymepedia Cohort

International audienceObjective: To evaluate cytomegalovirus (CMV) viral load dynamics in blood and saliva during the first two years of life in symptomatic and asymptomatic infected infants and to identify whether these kinetics could have practical clinical implications.Study design: The Cymepedia cohort prospectively included 256 congenitally infected neonates followed for two years. Whole blood and saliva were collected at inclusion, months 4 and 12, and saliva at months 18 and 24. Real-time CMV PCR was performed, results expressed as log10 IU/mL in blood and in copies/mL in saliva.Results: Viral load in saliva progressively decreased from 7.5 log10 at birth to 3.3 log10 at month 24. CMV PCR in saliva was positive in 100% and 96% of infants at 6 and 12 months, respectively. In the first month of life, neonatal saliva viral load <5 log10 was related to a late CMV transplacental passage. Detection in blood was positive in 92% (147/159) of neonates in the first month of life. No viral load threshold values in blood or saliva could be associated with a high risk of sequelae. Neonatal blood viral load <3 log10 IU/ml had a 100% negative predictive value (NPV) for long-term sequelae.Conclusions: Viral loads in blood and saliva by CMV PCR testing in congenital infection fall over the first 24 months. In this study of infants affected mainly after primary maternal infection during pregnancy, all salivary samples were positive in the first 6 months of life and sequelae were not seen in infants with neonatal blood viral load <3 log10 IU/mL

Prenatal education of overweight or obese pregnant women to prevent childhood overweight (the etoig study): an open-label, randomized controlled trial

Background We aimed to evaluate whether pre and perinatal education of pregnant women would reduce childhood overweight. Methods Four French centers included women at 25 kg/m(2) before pregnancy. Patients were randomized to a control group (routine care including at least one dietary visit) or an intervention group (2 individuals (26 and 30 GW) and 4 group sessions (21, 28, 35 GW, 2 months postpartum)) aimed at educating the future mother regarding infant and maternal nutrition. The primary objective was to reduce post-natal excessive weight gain in the infant from birth to 2 years (NCT00804765). This project was funded by a grant from the National Programme for Hospital Research (PHRC-2007 French Ministry of Health). Results We included 275 women (BMI: 32.5 kg/m(2)). The rate of post-natal excessive weight gain was similar in the intervention (n = 132) and control (n = 136) groups by intention to treat (ITT: 59.1% vs 60.3% respectively, p = 0.84) in available data (AD, n = 206) and by per-protocol analysis (PP, n = 177). Two years after delivery, normalization of maternal BMI and number of infants with BMI 19 kg/m(2) in the intervention group in AD (n = 204: 0% vs 6.8%, p = 0.014) and PP (n = 176: 0% vs 6.4%, p = 0.03). Conclusions An education and nutritional counseling program for overweight women, starting after 3 months of gestation, did not significantly change post-natal excessive weight gain of infants or prevent overweight in mothers and children 2 years after delivery