In vitro versus in situ biofilms for evaluating the antimicrobial effectiveness of herbal mouthrinses

For centuries, diverse mouthrinses have been applied for medicinal purposes in the oral cavity. In view of the growing resistance of oral microorganisms against conventional antimicrobial agents e.g. chlorhexidine, the implementation of alternative treatments inspired by nature has lately gained increasing interest. The aim of the present study was to compare in vitro biofilm models with in situ biofilms in order to evaluate the antimicrobial potential of different natural mouthrinses. For the in vitro study a six-species supragingival biofilm model containing A. oris, V. dispar, C. albicans, F. nucleatum, S. mutans and S. oralis was used. Biofilms were grown anaerobically on hydroxyapatite discs and treated with natural mouthrinses Ratanhia, Trybol and Tebodont. 0.9% NaCl and 10% ethanol served as negative controls, while 0.2% CHX served as positive control. After 64h hours, biofilms were harvested and quantified by cultural analysis CFU. For the in situ study, individual test splints were manufactured for the participants. After 2h and 72h the biofilm-covered samples were removed and treated with the mouthrinses and controls mentioned above. The biofilms were quantified by CFU and stained for vitality under the confocal laser scanning microscope. In the in vitro study, 0.2% CHX yielded the highest antimicrobial effect. Among all mouthrinses, Tebodont (4.708 ± 1.294 log10 CFU, median 5.279, p<0.0001) compared with 0.9% NaCl showed the highest antimicrobial potential. After 72h there was no significant reduction in CFU after 0.2% CHX treatment. Only Trybol showed a statistically significant reduction of aerobic growth of microorganisms in situ (5.331 ± 0.7350 log10 CFU, median 5.579, p<0.0209). After treatment with the positive control 0.2% CHX, a significant percentage of non-vital bacteria (42.006 ± 12.173 log10 CFU, median 42.150) was detected. To sum up, a less pronounced effect of all mouthrinses was shown for the in situ biofilms compared to the in vitro biofilms

Minimalinvasive Versorgung von Erosionen zur Wiederherstellung von Gesundheit, Funktion und Ästhetik mit indirekten Restaurationen

This article discusses the treatment of tooth structure loss due to erosion using indirect lithium disilicate ceramic restorations. Minimally invasive approaches, in which the eroded teeth are conservatively prepared and restored with minimally invasive restorations, are recommended. Lithium disilicate ceramics are currently the material of choice for this type of treatment as they can withstand maximum occlusal forces in the posterior region. The restorative process should be guided by diagnostic procedures that define the clinical therapeutic goal at the beginning of treatment. Adhesive cementation with the correct protocol is crucial for full mechanical strength of the restoration. At the end of the treatment, in addition to preventive measures, an overnight protective splint is recommended to ensure long-term clinical stability

Mechanical properties of toothpastes with diamond abrasives

Objective: The aim of this study was to evaluate and compare surface roughness, gloss, and color change of dental enamel after being brushed with toothpastes containing diamond powder and traditional abrasives. Materials and methods: Seventy enamel slabs were derived from 70 bovine incisors. The slabs were brushed with six different toothpastes and artificial saliva as a negative control. The specimens were then stained with black tea mixed with citric acid (3 days, pH = 4) and again brushed with the same toothpastes. Ra (contact profilometer), gloss (glossmeter), and color (CIE L* a* b* system) values were measured after each step. Results: Emoform-F Diamond (contains diamond powder and traditional abrasives) offered significantly the best improvement of Ra and gloss values after the first brushing sequence and the best recovery of the brightness of enamel after staining and second brushing sequence (P < .05). AMC 2.5 (contains only diamond powder as abrasive) was not able to offer such improvement. Conclusion: Diamond powder as an additional abrasive in toothpastes could be able to offer a further improvement of Ra , gloss, and color values of enamel. Keywords: abrasives; diamond powder; gloss; surface roughness; toothpastes

Salivary biomarkers as key to monitor personalized oral healthcare and precision dentistry: A scoping review

Personalized Oral Healthcare has recently become the new trend word in medicine and dentistry. In this context, saliva diagnostics using various biomarkers seem to be the gateway to personalized dental diagnostics and therapy. But the terminology is not (yet) uniformly defined, furthermore it is unclear to what extent which salivary markers play a relevant role in the therapeutic decision making. In this Scoping Review, an electronic search was conducted in PubMed and Web of Science databases using medical subject headings (MESH terms) "saliva", "biomarker", "personality/persons", and "dentistry". Only human studies were included, in which repeated salivary measurements were performed to analyze monitoring effects with at least ten patients per group. PRISMA-ScR and Tricco guidelines were followed: (i) to examine what salivary biomarkers have been explored in terms of personalized oral healthcare and precision dentistry, (ii) to investigate the clinical relevance for oral health and its correlation to systemic health, and (iii) to summarize an outlook for future developments based on these results. Out of 899 studies, a total of 57 were included for data extraction in this Scoping Review, mainly focusing on periodontal therapy and patient monitoring. Salivary biomarkers have shown the potential to change the field of dentistry in all dental disciplines as a key for personalized workflows. The increasing interest in dental research is obvious, demonstrated by the growing number of publications in recent years. At this time, however, the predominant discipline is periodontology, which allows biomarker-based monitoring of the disease prevention and progression. The studies included showed heterogeneous methods using manifolds biomarkers. Therefore, no uniformly accepted concept can be presented today. Further clinical research with well-defined outcomes including standardized procedures is necessary