9 research outputs found
Analysis of gene expression induced by chronic exposure in the Mediterranean shrub Phyllirea latifolia L. by cDNA-AFLP
Seedlings of Phillyrea latifolia L., a Mediterranean
shrub, were exposed for 90 days to 110 nl l â1 ozone (O3).
Comparison of the cDNA-amplified fragment length polymorphism
(cDNA-AFLP) patterns for treated and control plants allowed
the identification and cloning of 88 differential sequences
induced by O3. The differential expression of 67
cloned sequenceswas further confirmed byRT-PCR. The functions
of 36 cloned sequences, corresponding to seven of the
twelve gene functional classes of Arabidopsis, were presumed
on the basis of their homology with characterized gene sequences.
Ozone induction of genes homologous to 24 of the
clones has been reported in other plant species, whereas the induction
of the 12 remaining sequences has not been observed
before. Ozone activation of these newly identified genes could
be a result of the chronic exposure to low O3 concentration, because
in most previous studies, acute treatments, involving
high O3 dosages, were applied. Possible roles of the cloned sequences
in the response of P. latifolia to O3 and other causes of
oxidative stress are discussed
The psychology of recycled water: Factors predicting disgust and willingness to use
Water recycling is increasingly recognized as a critical strategy to maintain sustainable water supplies. Yet public acceptance of water recycling often lags behind. It is unclear the degree to which individuals are aware of the role of disgust in their decisions about recycled water, how important anticipated disgust is to willingness to use when controlling for other factors, and what the most effective method of presenting information about water recycling would be to decrease disgust reactions and increase willingness to use. We used a twoâpronged approach, combining a survey with openâended and psychometric measures with an experimental manipulation, in a U.S., webâbased sample (Nâ=â428). Only 2% of participants selfâidentified disgust as important to their decisions about recycled water. When measured directly using a Likert scale, however, anticipated disgust was the strongest predictor of willingness to use recycled water when controlling for individual differences that have been shown to impact willingness to use, including a subscale of individual pathogen disgust sensitivity. Finally, participants were exposed to an educational brochure about water reuse framed either affectively or cognitively or were shown a simple, neutral definition. Exposure to either the affectively or cognitively framed brochures lowered anticipated disgust, but did not significantly affect willingness to use recycled water compared to the neutral condition.
Key Points:
Hierarchical regression was used to model willingness to use recycled water (RW)
A psychometric measure of anticipated disgust was the most important variable in this model
Viewing an informational brochure about RW lowered anticipated disgust compared to a contro