The effect of the antioxidant drug U-74389G on endometrial edema during ischemia reperfusion injury in rats

The aim of this experiment was to study the effects of the antioxidant drug U-74389G on rat model, particularly in ischemia reperfusion (IR) protocol. The beneficial or other effects of that molecule were studied pathologically using endometrial edema (EE) lesions. Forty rats were used of mean weight 231.875 gr. EE lesions were evaluated 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D), with administration of the drug U-74389G in groups C and D. Results were that U-74389G administration non-significantly decreased without lesions the EE scores by 0.41 [-1.00 - 0.17] (p= 0.1607). This finding was in accordance with the results of Wilcoxon signed-rank test (p= 0.5229). Reperfusion time non-significantly increased without lesions the EE scores by 0.21 [-0.38 - 0.81] (p= 0.4701), also in accordance with Wilcoxon signed-rank test (p= 0.1022). However, U-74389G administration and reperfusion time together non-significantly decreased without lesions the EE scores by 0.17 [-0.53 - 0.18] (p= 0.3383). Results of this study indicate that U-74389G administration hardly non-significantly decreases without lesions the EE within short-term time context of 2 hours

The effect of erythropoietin on ?-glutamyltransferase during ischemia reperfusion injury in rats

The aim of this experimental study was to examine the effect of erythropoietin on rat model and particularly in an ischemia reperfusion (IR) protocol. The effect of that molecule was studied biochemically using blood mean ?-glutamyltransferase (?GT) levels. Materials and methods: 40 rats of mean weight 247.7 g were used in the study. ?GT levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reperfusion. Erythropoietin was administered only in groups C and D. Results were that Epo administration non-significantly decreased the ?GT levels by 12.70% +13.11% results of paired t-test (p= 0.3541). Reperfusion time kept non-significantly increased the ?GT levels by 6.35%+13.24% (P=0.6264). However, erythropoietin administration and reperfusion time together produced a non-significant combined effect in keeping decreased the ?GT levels by 4.62%+7.97% (P= 0.5534). Conclusions are that erythropoietin administration interacted or not with reperfusion time have non significant short term decreasing effects on ?GT levels

Akutan učinak antioksidantnog lijeka U-74389G kod srednjih razina crvenih krvnih tjelešaca za vrijeme hipoksije i ponovljene oksigenacije kod štakora

Aim: The aim of this experimental study was to examine the effect of the antioxidant drug “U-74389G”, on rat model and particularly in a hypoxia – reoxygenation protocol. The beneficial effect or non-effectiveness of that molecule was studied hematologically using blood mean corpuscular hemoglobin (MCH) levels. Methods: 40 rats of mean weight 231.875 gr were used in the study. MCH levels were measured 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D) with the administration of drug U-74389G in groups C and D. Results: The results were that U-74389G administration significantly increased the MCH levels by 2.40% ± 0.57% (p = 0.0001). Reoxygenation time non-significantly decreased the MCH levels by 0.48% ± 0.69 (p = 0.4103). However, U-74389G administration and reoxygenation time together significantly increased the MCH levels by 1.33% ± 0.36% (p = 0.0005). Conclusion: The results of this study indicate that U-74389G administration either alone or interacted with reoxygenation time has significant increasing short – term effects on the pathophysiology recovery of MCH values.Cilj: Cilj ove eksperimentalne studije je ispitatiučinakantioksidantnog lijeka “U-74389G”, na model štakora, a posebno u protokolu hipoksije i ponovljene oksigenacije. Blagotvoran učinak ili neučinkovitost navedene molekule ispitani su hematološki korištenjem srednjih razina crvenih krvnih tjelešaca (MCH). Metode: U studiji je korišteno 40 štakora srednje težine od 231,875 g. MCH razine izmjerene su 60 min nakon reperfuzije (skupine A i C) i 120 min nakon reperfuzije (skupine B i D) davanjem lijeka U-74389G skupinama C i D. Rezultati: Davanje lijeka U-74389G značajno je povećalo razine MCH za 2,40% ± 0,57% (p = 0,0001). Ponovljena oksigenacija smanjila je beznačajno razine MCH za 0,48% ± 0,69 (p = 0,4103). Davanje U-74389G tijekom ponovljene oksigenacije međutim, značajno je povećalo MCH razine za 1,33% ± 0,36% (p = 0,0005). Zaključak: Rezultati ove studije pokazuju da zasebno davanje U-74389G ili u interakciji s ponovljenom oksigenacijom, značajno povećava kratkotrajne učinke na oporavak patofiziološke razine MCH

The trends of the antioxidant drug “U-74389G” on potassium levels during hypoxia reoxygenation injury in rats

Background: This experimental study examined the trends of the antioxidant drug “U-74389G”, on a rat model and particularly in a hypoxia – reoxygenation (HR) protocol. The trends of that molecule were studied biochemically using blood mean potassium levels. Methods: 40 rats of mean weight 231.875 g were used in the study. Potassium (K+) levels were measured at 60 min of reoxygenation (groups A and C) and at 120 min of reoxygenation (groups B and D) with administration of the drug U-74389G in groups C and D. Results: U-74389G administration non significantly decreased the K+ levels by 2.14%+5.06% (p= 0.6730). Reoxygenation time non-significantly increased the K+ levels by 8.66%+4.85% (p= 0.0934). However, U-74389G administration and reoxygenation time together non-significantly increased the K+ levels by 2.07%+3.03% (P= 0.4853). Conclusions: U-74389G administration, reoxygenation time and their interaction have miscellaneous non significant short – term trends on potassium levels. Perhaps, a longer study time or a higher drug dose may reveal clearer and significant effects

The effect of erythropoietin on chloride levels during hypoxia reoxygenation injury in rats

Objective. This experimental study examined the effect of erythropoietin (Epo) in a rat model and particularly in a hypoxiareoxygenation (HR) protocol. The effect of that molecule was studied biochemically using blood mean chloride (Cl) levels. Materials and methods. 40 rats of mean weight 247.7 g were used in the study. Cl levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reoxygenation. Erythropoietin was administered only in groups C and D. Results. Epo administration non-significantly decreased Cl levels by 1.07%+0.91% (p=0.2635). Reoxygenation time nonsignificantly decreased Cl levels by 0.68%+0.92% (P= 0.4457). However, erythropoietin administration and reoxygenation time together produced a non-significant combined effect in decreasing Cl levels by 0.74%+0.54% (P= 0.1701). Conclusions. Epo administration, reoxygenation time and their interaction have non-significant, short-term, decreasing effects on Cl levels

Elevated Circulating IL-1β and TNF-Alpha, and Unaltered IL-6 in First-Trimester Pregnancies Complicated by Threatened Abortion With an Adverse Outcome

The purpose of the present study was to examine the profile of selected proinflammatory cytokines in maternal serum of first-trimester pregnancies complicated by threatened abortion (TACP) and its relevance to obstetric outcome. Serum levels of Th1-type cytokines interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-alpha), and Th2-type cytokine interleukin 6 (IL-6) were measured, by ELISA, in 22 women with TACP and adverse outcome at admission (group A) and compared with the corresponding levels of 31 gestational age-matched women with TACP and successful outcome at admission (group B1) and discharge (group B2) and 22 gestational age-matched women with first-trimester uncomplicated pregnancy (group C) who served as controls. Mann-Whitney U or Wilcoxon test was applied as appropriate to compare differences between groups. IL-1β and TNF-alpha were detected with significantly higher levels in group A, compared to all other groups. On the contrary, IL-6 levels were detected with no significant difference among all the other groups studied. It is concluded that in first-trimester TACP with adverse outcome, a distinct immune response, as reflected by elevated maternal IL-1β, TNF-alpha, and unaltered IL-6 levels, is relevant to a negative obstetric outcome

The effect of erythropoietin on endosalpingeal karyorrhexis during ischemia reperfusion injury in rats

Introduction. The aim of this experiment was to study the effects of the antioxidant drug “U-74389G” on rat model, particularly in ischemia reperfusion protocol. The beneficial or other effects of that molecule were studied estimating the mean endosalpingeal karyorrhexis (EK) lesions. Material and methods. 40 rats were used of mean weight 247.7 g. EK was evaluated 60 min after reperfusion for groups A and C and 120 min after reperfusion for groups B and D. Groups A and B without the drug but C and D with erythropoietin administration. Results. Results were that erythropoietin administration kept non-significantly increased the EK scores by 0.1 (–0.0393284–0.2393284) (p = 0.1544). This finding was in accordance with the results of Wilcoxon signed-rank test (p = 0.1573). Reperfusion time non-significantly decreased the EK scores by 0.1 (–0.2393284–0.0393284) (p = 0.1544), approximately in accordance with the increased EK score by 0.1 (–0.2440518–0.0440518) of Wilcoxon signed-rank test (p = 0.1573). However, Epo administration and reperfusion time together kept non-significantly increased the EK scores by 0.0181818 (–0.0679319–0.1042955) (p = 0.6715). Conclusions. Results of this study indicate that Epo administration kept non-significantly increased the EK scores. Perhaps, a longer study time than 2 hours may provide more significant effects.Due to impossible pasting, you are kindly asked to find the abstract in main documen

THE ACUTE EFFECT OF ERYTHROPOIETIN ON GLUCOSE LEVELS DURING ISCHEMIA REPERFUSION INJURY IN RATS

The aim of this experimental study was to examine the effect of erythropoietin on rat model and particularly in an ischemia reperfusion (HR) protocol. The effect of that molecule was studied biochemically using blood mean glucose (Gl) levels. Materials and methods: 40 rats of mean weight 247.7 g were used in the study. Gl levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reperfusion. Erythropoietin was administered only in groups C and D. Results were that Epo administration non-significantly increased the gl levels by 5.59% +6.46% (p=0.3208). Reperfusion time non-significantly increased the gl levels by 5.63%+6.45% (p=0.4098). However, erythropoietin administration and reperfusion time together produced a non significant combined effect in increasing the gl levels by 4.94%+3.81% (p= 0.1892). Conclusions: Results of this study indicate that erythropoietin administration, reperfusion time, or their interaction non-significantly increase the blood glucose levels in short-term. Opposite bibliographic data are considered more reliable, until a greater sample provide clearer results

The effect of the antioxidant drug “U-74389G†on creatine kinase - MB levels during ischemia reperfusion injury in rats

Background: This experimental study examined the effect of the antioxidant drug “U-74389Gâ€, on a rat model and particularly in a liver ischemia - reperfusion protocol. The effects of that molecule were studied biochemically using blood mean creatine kinase - MB (CK-MB) levels. Methods: 40 rats of mean weight 231.875 g were used in the study. CK-MB levels were measured at 60 min of reperfusion (groups A and C) and at 120 min of reperfusion (groups B and D). The drug U-74389G was administered only in groups C and D. Results: U-74389G administration kept significantly increased the CK-MB levels by 31.60% + 13.10% (p = 0.0148). Reperfusion time kept non-significantly increased the CK-MB levels by 5.40% + 14.09% (p= 0.6358). However, U-74389G administration and reperfusion time together kept non-significantly increased the CK-MB levels by 14.50% + 8.16% (p=0.0745). Conclusions: U-74389G administration reduced at non-significantly increased the CK-MB levels, getting on decline them from significant to non-significant level

The effect of erythropoietin on albumins levels during hypoxia reoxygenation injury in rats

The aim of this experimental study was to examine the effect of erythropoietin on rat model and particularly in an hypoxia reoxygenation (HR) protocol. The effect of that molecule was studied biochemically using blood mean albumins levels.Materials and methods: 40 rats of mean weight 247.7 g were used in the study. Albumins levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reoxygenation. Erythropoietin was administered only in groups C and D.Results were that Epo administration significantly decreased the albumins levels by 9.28%+3.20% (p=0.0054). Reperfusion time non-significantly increased the albumins levels by 3.09%+3.52%  (p= 0.3405). However, erythropoietin administration and reperfusion time together produced a significant combined effect in keeping decreased the albumins levels by 5.37%+2.73% (p= 0.0072).Conclusions are that erythropoietin administration whether it interacted or not with reoxygenation time, has significant decreasing effects on albumins levels in a short-term context of 2 hours