Utišani gga-miR-142-3p u pilećem embriju: ekspresija profila XPO1.

Differential expression of gga-miR-142-3p microRNA of haemopoeitic origin during immune organ development and functional stages in chicken embryos creates new opportunities for understanding its pivotal role during embryonic developmental stages. To decipher the role of gga-miR-142-3p in-ovo knockdown was carried out with LNA modified anti-miR-gga-miR-142-3p via intravenous route at developmental and functional stages of the immune organs and other organs. Bioinformatic analysis of the genes targeted by gga-miRNA-142-3p revealed that the predicted gene XPO1 conserved binding sites at 3’UTR. The target gene XPO1 was evaluated as the validated target of gga-miR-142-3p by employing qPCR SYBR green based technology, which was evidenced by its increased expression in the tissues of gga-miR-142-3p knockdown chicken embryos. Histopathological alterations in the immune organs and visceral organs indicated that the systemic knockdown of gga-miR-124-3p led to over expression of the XPO1 gene during the embryonic stages, and changed the environment of the immune organs related to structural integrity, immune response, signal transduction and migration of B and T cells during the embryonic developmental stage in the chicken embryos. The results clearly indicated that these changes could alter the postnatal development and functions of these immune organs, and may lead to development of immuno-compromised chickens.Različita ekspresija gga-miR-142-3p mikroRNA hemopoetskog podrijetla daje nove mogućnosti razumijevanja njezine ključne uloge u embrionalnom razvoju limfnih organa i funkcionalnih zbivanja u pilećem zametku. Za otkrivanje uloge gga-miR-142-3p in ovo provedeno je utišavanje zaključanom nukleinskom kiselinom što preko anti-miR-gga-miR-142-3p preinačuje razvojne i funkcionalne sposobnosti limfnih i drugih organa. Bioinformatička analiza ciljnih gena za gga-miRNA-142-3p otkriva da gen XPO1 ima konzervirana mjesta vezanja na 3’UTR. Gen XPO1 bio je potvrđen kao cilj za gga-miR-142-3p uporabom tehnologije temeljene na qPCR SYBR zelenilu, što je bilo dokazano njegovom povećanom ekspresijom u tkivima pilećih zametaka s utišanim gga-miR-142-3p. Patohistološke promjene u imunosnim i unutarnjim organima pokazuju da sustavno utišavanje gga-miR-124-3p vodi do prevelike ekspresije gena XPO1 tijekom embrionalnog razvoja. To mijenja zadaću imunosnih organa s obzirom na strukturni integritet, imunosni odgovor, prijenos poruka te migraciju B i T limfocita tijekom razvoja pilećih zametaka. Rezultati jasno naznačuju da te promjene mogu preinačiti postnatalni razvoj i funkcije imunosnih organa te mogu dovesti do razvoja imunološki oslabljenih pilića