1 research outputs found
Acinetobacter junii AH4-A Potential Strain for Biohydrogen Production from Dairy Industry Anaerobic Sludge
The present study aims to enhance the efficiency of anaerobic sludge microorganisms to produce hydrogen (H2
)
through various pre-treatment methods. The various pre-treatment methods such as base, acid, chloroform, heat
shock, freezing and thawing have enabled to isolate acidogenic bacteria with higher bio H2
producing activity in an
anoxic environment. From these various treatments, bacteria were isolated and screened for bio H2
capabilities.
Among the one bacterial strain, AH4 strain showed maximum cumulative H2
production and Hydrogen Yield (HY)
using 100% diary anaerobic sludge. AH4 strain was identified as Acinetobacter junii using 16S rRNA gene sequence
and used for further experimental analysis. Biohydrogen productions of Acinetobacter junii were measured at
different experimental setup such as various pH levels (5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0) and different substrate
concentration (10 - 100%) of dairy anaerobic sludge substrate. At pH 7.5 and 60% substrate concentration, the strain
AH4 Acinetobacter junii displayed the maximum cumulative H2
production of 945.7 ml/L and H2
yield 1.35 mol H2
/
mol glucose. Based on our results, we concluded that Acinetobacter junii can be used as a promising bio agent for
hydrogen production on a large scale using dairy anaerobic sludge as substrate