Evaluation of bacteriological profile and antibiotic susceptibility pattern of patients with otorrhea in a tertiary care teaching hospital

Background: Otitis media is very common disease in developing country and if not treated properly can lead to hearing loss and serious neurological complications. Its bacteriology and antibiotic sensitivity varies in different population. Thus knowledge of the microbiological pattern with antibiotic susceptibility is important to deliver efficacious treatment of this disease. So, purpose of the present study was to determine the microbiological profile and antimicrobial susceptibility pattern of isolates from ear discharge in patients of otitis media.Methods: This study was conducted in Department of Microbiology of our institute for duration of one year. A total of 100 patients’ samples visiting ENT OPD were taken who complaining of ear discharge. Identification of organism was done by using standard biochemical reactions and antibiotic susceptibility testing done by using modified Kirby Bauer method as per CLSI guidelines.Results: Majority of the patients were between 21-30 years of age group (28%). The most common organism isolated was Pseudomonas aeruginosa (25.88%), highly sensitive to aminoglycosides and β-lactam + β-lactamase inhibitor (100%) followed by Staphylococcus aureus (21.17%), highly sensitive to aminoglycosides (100%) and fluoroquinolones (72.22%).Conclusions: Knowledge of the pathogenic agents responsible for otitis media and choice of effective antibiotics according to susceptibility pattern will guide the treatment. It also helps in reducing complications of the disease and decreasing emergence of resistance to antibiotics.

A study of prevalence and seasonal trends of different malarial species in district hospital

Background: Malaria imposes great socio-economic burden on humanity. In India, the epidemiology of malaria is complex because of wide distribution of anopheline vectors which transmitting mainly two Plasmodial species named P. falciparum and P. vivax. Though India is one of the known endemic countries, incidence of malaria is commonly influenced by environmental factors like climate, season, temperature and socioeconomic status. Objectives of the study were to know the prevalence of different malarial species and to know the seasonal trend of malaria.Methods: This study done microbiology department of district hospital over period of June 2015 to December 2015. We had collected total 6763 samples. We used Microscopic examination for the diagnosis of malaria by preparing thick and thin smears and stained using field stain.Results: We had collected total 6763 samples out of which 108 samples are found microscopically positive (1.5%). In our hospital we observed only Plasmodium vivax and Plasmodium falciparum spp. Prevalence of P. vivax (64%) found more compare to P. falciparum (34%) and peak positivity rate found in July to October.Conclusions: In our study the most frequently implicated species was P. vivax. This indicates that P. vivax is the most widespread infection in India which results in a pronounced morbidity and the seasonal prevalence observed high in July to October. In order to implement effective preventive measures, proper surveillance on the incidence and prevalence of malaria is required