Digraphs with degree equivalent induced subdigraphs

AbstractA digraph G of order n is said to have property Dk if every induced subdigraph of order n − k in G has the same degree sequence. In this paper, we characterize all digraphs with property Dk when k = 1, 2

Genomic and proteomic profiling I: Leiomyomas in African Americans and Caucasians

<p>Abstract</p> <p>Background</p> <p>Clinical observations indicate that leiomyomas occur more frequently in African Americans compared to other ethnic groups with unknown etiology. To identify the molecular basis for the difference we compared leiomyomas form A. Americans with Caucasians using genomic and proteomic strategies.</p> <p>Methods</p> <p>Microarray, realtime PCR, 2D-PAGE, mass spectrometry, Western blotting and immunohistochemistry.</p> <p>Results</p> <p>Using Affymetrix U133A array and analysis based on P ranking (P < 0.01) 1470 genes were identified as differentially expressed in leiomyomas compared to myometrium regardless of ethnicity. Of these, 268 genes were either over-expressed (177 genes) or under-expressed (91 genes) based on P < 0.01 followed by 2-fold cutoff selection in leiomyomas of A. Americans as compared to Caucasians. Among them, the expression E2F1, RUNX3, EGR3, TBPIP, ECM2, ESM1, THBS1, GAS1, ADAM17, CST6, CST7, FBLN5, ICAM2, EDN1 and COL18 was validated using realtime PCR low-density arrays. 2D PAGE coupled with image analysis identified 332 protein spots of which the density/volume of 31 varied by greater than or equal to 1.5 fold in leiomyomas as compared to myometrium. The density/volume of 34 protein-spots varied by greater than or equal to 1.5 fold (26 increased and 8 decreased) in leiomyomas of A. Americans as compared to Caucasians. Tandem mass spectrometric analysis of 15 protein spots identified several proteins whose transcripts were also identified by microarray, including 14-3-3 beta and mimecan, whose expression was confirmed using western blotting and immunohistochemistry.</p> <p>Conclusion</p> <p>These findings imply that the level rather than the ethnic-specific expression of a number of genes and proteins may account for the difference between leiomyomas and possibly myometrium, in A. Americans and Caucasians. Further study using larger sample size is required to confirm these findings.</p

Genomic and proteomic profiling II: Comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars

<p>Abstract</p> <p>Background</p> <p>Leiomyoma have often been compared to keloids because of their fibrotic characteristic and higher rate of occurrence among African Americans as compared to other ethnic groups. To evaluate such a correlation at molecular level this study comparatively analyzed leiomyomas with keloids, surgical scars and peritoneal adhesions to identify genes that are either commonly and/or individually distinguish these fibrotic disorders despite differences in the nature of their development and growth.</p> <p>Methods</p> <p>Microarray gene expression profiling and realtime PCR.</p> <p>Results</p> <p>The analysis identified 3 to 12% of the genes on the arrays as differentially expressed among these tissues based on P ranking at greater than or equal to 0.005 followed by 2-fold cutoff change selection. Of these genes about 400 genes were identified as differentially expressed in leiomyomas as compared to keloids/incisional scars, and 85 genes as compared to peritoneal adhesions (greater than or equal to 0.01). Functional analysis indicated that the majority of these genes serve as regulators of cell growth (cell cycle/apoptosis), tissue turnover, transcription factors and signal transduction. Of these genes the expression of E2F1, RUNX3, EGR3, TBPIP, ECM-2, ESM1, THBS1, GAS1, ADAM17, CST6, FBLN5, and COL18A was confirmed in these tissues using quantitative realtime PCR based on low-density arrays.</p> <p>Conclusion</p> <p>the results indicated that the molecular feature of leiomyomas is comparable but may be under different tissue-specific regulatory control to those of keloids and differ at the levels rather than tissue-specific expression of selected number of genes functionally regulating cell growth and apoptosis, inflammation, angiogenesis and tissue turnover.</p

A Mixing Coupling Scheme for Spectra of Singly Heavy Baryons with Spin-1 Diquarks in P-waves

A new scheme of state classification is proposed and applied to analyze masses of the heavy baryons $\Omega_{Q}$, $\Sigma_{Q}$ and $\Xi_{Q}^{\prime}$ in P-waves. The results confirm all excited $\Omega_{c}$ and $\Omega_{b}$ baryons reported recently by LHCb to be bound states of a P-wave $ss$-diquark and a respective charm or bottom quark, and thereby predict Regge trajectories for more excited $\Omega_{c}$ and $\Omega_{b}$ baryons. We suggest one excited {$J^{P}=5/2^{-}$} $\Omega_{b}$ state to be unseen by LHCb around $6352$ MeV, and predict P-wave masses of all spin-partners of the odd-parity baryons $\Sigma_{c}(2800)/\Xi_{c}^{\prime}(2942)$ and $\Sigma_{b}(6097)$/$\Xi_{b}^{\prime }(6227)$. A computation is further given in a relativized potential quark models to explain matched values of spin couplings of all considered baryons, by which a scaling law for these spin couplings is discussed.Comment: 28 pages, 3 figures in total, with one new figure (FIG. 3) and one new section (Section VI) added in this enlarged version(V3). Due to twice extensions (Section V and Section VI added) including two newly added figures(FIG. 2 and FIG. 3), we also changed the title correspondingl

Inhibition of hepatocelluar carcinoma MAT2A and MAT2beta gene expressions by single and dual small interfering RNA

RNA interference (RNAi) has been successfully applied in suppression of hepatic cancer genes. In hepatocelluar carcinoma cell, one methionine adenosyltransferase (MAT) isozyme, MATII was found to have two catalytic subunits which were encoded by MAT2A and MAT2β respectively. During tumorigeness of hepatocelluar carcinoma, expressions of the two genes were discovered to be increased combining with a switch of MAT (form MATI to MATII), To figure out the role played by MATII in hepatic cancer, In this study, for the first time we established a dual small interfering RNA (siRNA) expression system, which could simultaneously express two different siRNA molecules specifically targeting two genes. To test the effectiveness of this system, we applied this approach to express simultaneously two different siRNA duplexes that specifically target MAT2A and MAT2β genes of hepatocelluar carcinoma respectively in HepG2 cell. Results indicated that dual siRNA could simultaneously inhibit the expression of MAT2A and MAT2β gene by 89.5% and 97.8% respectively, In addition, dual siRNA molecules were able to significantly suppress growth of hepatocelluar carcinoma cell in vitro as well as induce apoptosis which was involved in arrest cell cycle at the G1/S checkpoint and the expressions of p21, p27 and Bax