3, 4-dihydroxyl-phenyl lactic acid restores NADH dehydrogenase 1 α subunit 10 to ameliorate cardiac reperfusion injury.

The present study aimed to detect the role of 3, 4-dihydroxyl-phenyl lactic acid (DLA) during ischemia/reperfusion (I/R) induced myocardial injury with emphasis on the underlying mechanism of DLA antioxidant. Male Spragu-Dawley (SD) rats were subjected to left descending artery occlusion followed by reperfusion. Treatment with DLA ameliorated myocardial structure and function disorder, blunted the impairment of Complex I activity and mitochondrial function after I/R. The results of 2-D fluorescence difference gel electrophoresis revealed that DLA prevented the decrease in NDUFA10 expression, one of the subunits of Complex I. To find the target of DLA, the binding affinity of Sirtuin 1 (SIRT1) to DLA and DLA derivatives with replaced two phenolic hydroxyls was detected using surface plasmon resonance and bilayer interferometry. The results showed that DLA could activate SIRT1 after I/R probably by binding to this protein, depending on phenolic hydroxyl. Moreover, the importance of SIRT1 to DLA effectiveness was confirmed through siRNA transfection in vitro. These results demonstrated that DLA was able to prevent I/R induced decrease in NDUFA10 expression, improve Complex I activity and mitochondrial function, eventually attenuate cardiac structure and function injury after I/R, which was possibly related to its ability of binding to and activating SIRT1

Sentinel lymph node biopsy as guidance for radical trachelectomy in young patients with early stage cervical cancer

<p>Abstract</p> <p>Background</p> <p>The purpose of this study was to assess the feasibility and accuracy of sentinel lymph nodes (SLNs) detection using 99mTc phytate in predicting pelvic lymph nodes status for radical abdominal trachelectomy (RAT) in patients with early stage cervical cancer.</p> <p>Methods</p> <p>Sixty-eight women with stage IA2-IB1 cervical cancer and scheduled to undergo fertility-sparing surgery enrolled in this study. 99mTc-labeled phytate was injected before surgery. Intraoperatively, SLNs were identified, excised, and submitted to fast frozen section. Systematic bilateral pelvic lymphadenectomy and/or para-aortic lymph node dissection was performed. Then RAT was performed in patients with negative SLNs. All nodes were sent for routine pathological examination and immunostained with anti-cytokeratin antibody to detect micrometastases. Outcomes of follow up and fertility were observed.</p> <p>Results</p> <p>SLNs were identified in 64 of 68 patients (94.1%). Of these, SLNs of 8 patients (11.8%) were positive on frozen sections and proved to be metastasis by final pathologic examination. The sensitivity, accuracy, and false negative rates were 100%, 100%, and 0%, respectively. All 60 patients with negative SLN underwent RAT successfully. Two relapses occurred and no one died of tumor progression during follow-up. Five of the 15 patients with procreative desire conceived 8 pregnancies (3 term delivery, 2 premature birth, 1 spontaneous abortion, and 2 were still in the duration of pregnancy) after surgery.</p> <p>Conclusions</p> <p>The identification of SLN using 99mTc-labeled phytate is accurate and safe to assess pelvic nodes status in patients with early cervical cancer. SLNs biopsy guided RAT is feasible for patients who desire to have fertility preservation.</p

Progression of the Wei-Qi-Ying-Xue syndrome, microcirculatory disturbances, in infectious diseases and treatment with traditional Chinese medicine

Lipopolysaccharide (LPS)-induced endotoxemia is a critical condition that initiates microcirculatory disturbance and may progress to multiple organ failure that threatens the lives of millions of people around the world each year. The pathology of endotoxemia involves multiple insults mediated by a range of signaling pathways. Multitarget management is required to relieve endotoxemia. Traditional Chinese medicine (TCM) is a type of therapeutic that commonly contains numerous components and, thus, exhibits multitarget potential. More importantly, some TCM formulas have been proposed and used for effective treatment of endotoxemia-like diseases. In the past 20 years, an increasing number of studies have explored the effects and mechanisms of these formulas and their major bioactive components on microcirculatory disturbance and organ injury caused by LPS. The results obtained thus far provide support for the clinical use of TCM and shed light on the underlying mechanisms

The Contribution of Different Components in QiShenYiQi Pills® to Its Potential to Modulate Energy Metabolism in Protection of Ischemic Myocardial Injury

Ischemic heart diseases remain a challenge for clinicians. QiShenYiQi pills® (QSYQ) has been reported to be curative during coronary heart diseases with modulation of energy metabolism as one of the underlying mechanisms. In this study, we detected the effect of QSYQ and its components on rat myocardial structure, mitochondrial respiratory chain complexes activity and energy metabolism, and heart function after 30 min of cardiac ischemia, with focusing on the contribution of each component to its potential to regulate energy metabolism. Results showed that treatment with QSYQ and all its five components protected myocardial structure from damage by ischemia. QSYQ also attenuated release of myocardial cTnI, and restored the production of ATP after cardiac ischemia. AS-IV and Rb1, but not Rg1, R1, and DLA, had similar effect as QSYQ in regulation of energy metabolism. These results indicate that QSYQ may prevent ischemia-induced cardiac injury via regulation of energy metabolism, to which each of its components contributes differently

Combination of soy protein isolate and calcium chloride inhibits browning and maintains quality of fresh-cut peaches

Fresh-cut fruits are often more perishable and exhibit browning shortly after pulp is cut and exposed to natural atmosphere. In this study, fresh-cut peaches were treated with distilled water (control),  20 g L-1 soy protein isolate (SPI), 10 g L-1 calcium chloride (CaCl2) or 20 g L-1 soy protein  isolate + 10 g L-1 calcium chloride (20 g L-1 SPI + 1.0 g L-1 CaCl2), respectively, and then stored at 0°C to assess the protective effects of these treatments on the qualities and the antioxidative system of fresh-cut peaches. The result showed that firmness, colour(∆E*) and soluble solid content (SSC) in fresh-cut peaches treated with 20 g L -1 SPI + 10 g L-1 CaCl2 were higher than control. Compared with other treatments, treatment with 20 g L-1 SPI + 10 g L-1 CaCl2 2 significantly decreased weight loss rate, bacterial growth, respiratory rate and ethylene emission of fresh-cut peaches. Treatment with 20 g L-1 SPI + 10 g L-1 CaCl2 also inhibited increase in browning degree, production of reactive oxygen species, malonaldehyde and total phenols, and maintained higher activities of superoxide dismutase and catalase, whereas inhibited the activities of peroxidase and polyphenol oxidase. These results suggest that treatment with 20 g L-1 SPI + 10 g L-1 CaCl2 enhanced antioxidant activities of fresh-cut peaches, inhibited browning, and was more effective in maintaining the quality of fresh cut peaches.Keywords: Edible coating; Ethylene emission; Phenolic contents; Respiration rate; Active oxygen specie

Combination of soy protein isolate and calcium chloride inhibits browning and maintains quality of fresh-cut peaches

Fresh-cut fruits are often more perishable and exhibit browning shortly after pulp is cut and exposed to natural atmosphere. In this study, fresh-cut peaches were treated with distilled water (control),  20 g L-1 soy protein isolate (SPI), 10 g L-1 calcium chloride (CaCl2) or 20 g L-1 soy protein  isolate + 10 g L-1 calcium chloride (20 g L-1 SPI + 1.0 g L-1 CaCl2), respectively, and then stored at 0°C to assess the protective effects of these treatments on the qualities and the antioxidative system of fresh-cut peaches. The result showed that firmness, colour(∆E*) and soluble solid content (SSC) in fresh-cut peaches treated with 20 g L -1 SPI + 10 g L-1 CaCl2 were higher than control. Compared with other treatments, treatment with 20 g L-1 SPI + 10 g L-1 CaCl2 2 significantly decreased weight loss rate, bacterial growth, respiratory rate and ethylene emission of fresh-cut peaches. Treatment with 20 g L-1 SPI + 10 g L-1 CaCl2 also inhibited increase in browning degree, production of reactive oxygen species, malonaldehyde and total phenols, and maintained higher activities of superoxide dismutase and catalase, whereas inhibited the activities of peroxidase and polyphenol oxidase. These results suggest that treatment with 20 g L-1 SPI + 10 g L-1 CaCl2 enhanced antioxidant activities of fresh-cut peaches, inhibited browning, and was more effective in maintaining the quality of fresh cut peaches.Keywords: Edible coating; Ethylene emission; Phenolic contents; Respiration rate; Active oxygen specie

Panax Notoginseng Saponins Restrains Ischemia-reperfusion-induced Rat Mesenteric Microcirculatory Disturbance

Objective: To investigate the effect of Panax notoginseng saponins (PNS) on ischemia reperfusion (I/R) induced rat mesenteric microcirculatory dysfunctions