The adherent/invasive escherichia coli (AIEC) strain LF82 invades and persists in human prostate cell lineRWPE-1 activating a strong inflammatory response

Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect prostate cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 prostate cell line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within prostate epithelial cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary trac

DPYD c.1905+1G>A and c.2846 A>T and UGT1A1*28 allelic variants as predictors of toxicity: Pharmacogenetic translational analysis from the phase III TRIBE study in metastatic colorectal cancer.

Abstract not availabl

EPMA-World Congress 2015: Bonn, Germany. 3-5 September 2015

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Electrochemically induced diastereoselective functionalization of N-acyloxazolidin-2-ones

The electrochemical generation of chiral enolates is here described, either by cathodic reduction of a C-Br bond or using electrogenerated acetonitrile anion. The reactivity of these enolates have been studied in carboxylation, alkylation and conjugate addiction reactions, obtaining in some cases a good diastereoselectivity

Electrochemically induced Knoevenagel condensation in solvent- and supporting electrolyte-free conditions

Solvent- and supporting electrolyte-free electrochemically induced Knoevenagel condensation of malononitrile with aldehydes or ketones, at 40 uC, yields ylidenemalononitriles in high yields

A safe and mild synthesis of organic carbonates from alkyl halides and tetrabutylammonium alkyl carbonates

A safe and mild procedure for the synthesis of mixed organic carbonates is described. Reaction of com- mercially available tetrabutylammonium methoxide and ethoxide with carbon dioxide yields the corresponding meth- yl and ethyl tetrabutylammonium carbonates (TBAMC and TBAEC). The reactions of these new compounds with several different alkyl halides give methyl and ethyl carbonates in high yields. The use of classic toxic and harmful chemicals such as phosgene and carbon monoxide is avoided

Electrochemical carboxylation of N-(2-bromopropionyl)-4R-phenyloxazolidin-2-one: an efficient route to unsymmetrical methylmalonic ester derivatives

Electrochemical carboxylation of N-(2-bromopropionyl)-4R-phenyloxazolidin-2-one aimed at the synthesis and chiral resolution of unsymmetrical methylmalonic ester derivatives is described. The presence of the Evans’ chiral auxiliary permits an easy resolution of the mixture of the two diastereoisomers

An innovative strategy for electrochemically-promoted addition reaction

A new strategy based on the catalytic release of the supporting electrolyte agent in the electrolysis medium proved to be effective for the direct electroactivation of suitable C–H acid- containing compounds vs. catalytic addition processes, under solvent-free conditions

Electrochemical generation of chiral oxazolidin-2-ones anions: a new procedure for the highly diastereoselective conjugate addition to nitroalkenes

A mild and efficient electrochemical alternative to classical base-catalyzed conjugate addition of nitrogen nucleophiles is reported here. The cleavage of the carbamic N . H bond of Evans' chiral auxiliaries can be very efficiently performed by electrolysis under galvanostatic control and the resulting naked anions used for highly, diastereoselective conjugate addition to nitroalkenes. The degree of stereoselectivity was shown to depend on the steric hindrance of the group at the ring C(4) of the starting oxazolidin-2-one. (C) 2001 Elsevier Science Ltd. All rights reserved