Histomorphometric evaluation of bone healing in rabbit fibular osteotomy model without fixation

<p>Abstract</p> <p>Background</p> <p>Animal models of fracture consolidation are fundamental for the understanding of the biological process of bone repair in humans, but histological studies are rare and provide only qualitative results. The objective of this article is to present the histomorphometric study of the bone healing process using an experimental model of osteotomy in rabbit fibula without interference of synthesis material.</p> <p>Methods</p> <p>Fifteen rabbits were submitted to fibular osteotomy without any fixation device. Groups of five animals were submitted to pharmacological euthanasia during a period of one (group A), two (group B) and four weeks (group C) after osteotomy. Histomorphometric evaluation was performed in the histological sections.</p> <p>Results</p> <p>During week one there was intense cellularity (67/field), a large amount of woven bone (75.7%) and a small amount of lamellar bone (7.65%). At two weeks there was a decrease in woven bone (41.59%) and an increase in lamellar bone (15.16%). At four weeks there was a decrease of cellularity (19.17/field) and lamellar bone (55.56%) exceeded the quantity of woven bone (31.68%).</p> <p>Conclusion</p> <p>Histomorphometric (quantitative) evaluation of the present study was shown to be compatible with bone healing achieved in qualitative experimental models that have been commended in the literature.</p

Effect of polymorphisms in the Slc11a1 coding region on resistance to brucellosis by macrophages in vitro and after challenge in two Bos breeds (Blanco Orejinegro and Zebu)

The resistance/susceptibility of selected cattle breeds to brucellosis was evaluated in an F1 population generated by crossing animals classified as resistant (R) and susceptible (S) (R x R, R x S, S x R, S x S) based on challenges in vitro and in vivo. The association between single nucleotide polymorphisms identified in the coding region of the Slc11a1 gene and resistance/susceptibility was estimated. The trait resistance or susceptibility to brucellosis, evaluated by a challenge in vitro, showed a high heritable component in terms of additive genetic variance (h2 = 0.54 ± 0.11). In addition, there was a significant association (p < 0.05) between the control of bacterial survival and two polymorphisms (a 3'UTR and SNP4 located in exon 10). The antibody response of animals classified as resistant to infection by Brucella abortus differed significantly (p < 0.05) from that of susceptible animals. However, there was no significant association between single nucleotide polymorphisms located in the Slc11a1 gene and the antibody response stimulated by a challenge in vivo

Relação entre a competência funcional da memória episódica e os fatores associados à independência funcional de idosos saudáveis

OBJETIVO: O objetivo deste estudo foi descrever a rela&#231;&#227;o entre compet&#234;ncia funcional da mem&#243;ria epis&#243;dica e fatores associados &#224; independ&#234;ncia de idosos saud&#225;veis do munic&#237;pio de Juiz de Fora, Minas Gerais, Brasil. M&#201;TODOS: Trata-se de estudo transversal, descritivo e explorat&#243;rio em que 60 idosos saud&#225;veis compuseram a amostra final. Para avaliar a capacidade funcional, foram utilizadas as escalas de Katz e de Lawton & Brody. A mem&#243;ria epis&#243;dica foi avaliada utilizando-se teste de West & Thorn, validado e adaptado para a popula&#231;&#227;o brasileira por Yassuda, Lasca e Neri em 2005. A an&#225;lise estat&#237;stica foi realizada por meio do software estat&#237;stico Sphinx L&#233;xica e Eureka, vers&#227;o 5.0. RESULTADOS: Foi encontrada associa&#231;&#227;o estatisticamente significante entre escolaridade e desempenho em tarefa de mem&#243;ria epis&#243;dica e entre esta e tr&#234;s itens da escala de avalia&#231;&#227;o das atividades instrumentais de vida di&#225;ria, embora inconclusiva. CONCLUS&#213;ES: Acredita-se que esses resultados se devam ao fato de existir muitas diferen&#231;as entre os estudos com rela&#231;&#227;o &#224; metodologia empregada e a escolha dos instrumentos que avaliam tanto a mem&#243;ria epis&#243;dica, quanto a capacidade funcional de idosos. Al&#233;m disso, a hip&#243;tese de que no envelhecimento saud&#225;vel existe uma capacidade de, frente &#224;s perdas graduais na mem&#243;ria epis&#243;dica, compens&#225;-las utilizando outras estrat&#233;gias e recursos, deve receber aten&#231;&#227;o de futuras pesquisas