Rooting enhancers in the production of bougainvillea seedlings (Bougainvillea SP.)

This work aimed to evaluate the effect of different rooting enhancers on the development of Bougainvillea cuttings (Bougainvillea spetabilis Willd), aiming to accelerate rhizogenesis for seedling production. Semi-hardwood cuttings standardized in fifteen centimeters in length and diameter of approximately one centimeter were used. After collected, one third of the basal part of cuttings was immersed according to the following treatments: water (control); commercial product (RADIMAXi 20® at concentrations: Ca 25.6%, S 1.8%, Zn 2.5%, Co 1.5%); coconut water (Cocos nucifera L.); Tiririca extract (Cyperus rotundus L.) and Lentil extract (Lensculinaris Medik), for about two seconds. After immersion, cuttings were planted in polyethylene bags filled with 50% of Carolina Soil® commercial substrate and 50% of washed sand, packed under screen (50%) with a micro sprinkler irrigation system. The experimental design used was completely randomized, with five treatments and ten replicates. Results were submitted to analysis of variance and the means of treatments to Student\u27s t test at 5% probability. Evaluations were carried out at 45 days after installation and variables analyzed were: number of sprouts per cutting, length of the largest sprout, percentage of rooted cuttings and non-rooted live cuttings, length of the largest root, fresh and dry matter of roots, fresh and dry matter of shoots. Treatments that showed statistical difference were lentil extract, with the highest number of sprouts and the highest percentage of rooted cuttings and non-rooted live cuttings, and treatment with coconut water obtained the highest result in relation to variable length of the largest sprout, standing out as a promising sustainable alternative in the vegetative production of Bougainvillea seedlings

Characterization of Highly Weathered Soils

Os solos brasileiros são classificados em 13 ordens, com base em suas características definidoras. Os latissolos e os ultissolos predominam, compreendendo mais de 50% de todo o território. Portanto, o objetivo deste trabalho foi estudar os principais atributos físicos do solo e o conteúdo de matéria orgânica para avaliar as atribuições e limitações de dois tipos de solos altamente intemperizados no extremo oeste de São Paulo. O delineamento experimental foi inteiramente casualizado, composto por dois tratamentos diferentes: Latossolo e Ultisol em três faixas de profundidade: 0,00-0,10, 0,10-0,20, 0,20-0,40 m, com 10 repetições. As análises foram realizadas em triplicado. Os seguintes atributos do solo foram avaliados: textura do solo, conteúdo de matéria orgânica, umidade volumétrica e gravimétrica, infiltração de água no solo, densidade do solo, porosidade do solo, distribuição e estabilidade dos agregados, resistência à penetração e umidade do solo. Os dados foram analisados ​​quanto à variância com o teste F, a p ≤ 0,05. Quando significativos, os parâmetros foram submetidos ao teste de Tukey (p <0,05). As propriedades físicas do solo mostram que os solos estudados estão em boas condições e dentro dos limites médios recomendados pela literatura estabelecida. Todos os atributos estudados estão relacionados à granulometria do solo e sua distribuição no perfil do solo

A meta-analysis reveals the protein profile associated with malignant transformation of oral leukoplakia

The search for biomarkers associated with oral leukoplakia malignant transformation is critical for early diagnosis and improved prognosis of oral cancer patients. This systematic review and meta-analysis aimed to assess protein-based markers potentially associated with malignant transformation of oral leukoplakia. Five database and the grey literature were searched. In total, 142 studies were included for qualitative synthesis, where 173 proteins were investigated due to their potential role in malignant progression from oral leukoplakia (OL) to oral squamous cell carcinoma (OSCC). The abundance of these proteins was analyzed in fixed tissues and/or biofluid samples, mainly by immunohistochemistry and ELISA, and 12 were shared by both samples. Enrichment analysis revealed that the differential abundant proteins are mostly involved with regulation of cell death, regulation of cell proliferation, and regulation of apoptotic process. Also, these proteins are mainly expressed in the extracellular region (55.5%), cell surface (24.8%), and vesicles (49.1%). The meta-analysis revealed that the proteins related to tumor progression, PD-L1, Mdm2, and Mucin-4 were significantly associated with greater abundance in OSCC patients, with an Odds Ratio (OR) of 0.12 (95% CI: 0.04–0.40), 0.44 (95% CI: 0.24–0.81), and 0.18 (95% CI: 0.04–0.86), respectively, with a moderate certainty of evidence. The results indicate a set of proteins that have been investigated across OSCC initiation and progression, and whose transcriptional expression is associated with clinical characteristics relevant to the prognosis and aggressiveness. Further verification and validation of this biomarkers set are strongly recommended for future clinical application

Levantamento de fungos fitopatogênicos em solo de uma área a ser implantada uma horta

Brazil is a major producer of food and agricultural inputs in the world. One of the major problems is on plants in the occurrence of diseases, which can dramatically decrease the production of a culture. The success of a plant disease management strategy is dependent on factors involving knowledge of plant pathogens and potential in the fact that affect these diseases. Thus the study aimed to carry out the survey of phytopathogenic fungi in an area that will be deploying a dedicated garden for the people of Dracena. The soil analysis was based on the methodology of serial dilutions. Dilution factor of 1: 1000 were withdrawn aliquots of 1.0 mL, which were placed in petri dishes containing culture medium with 2% agar. The plates were incubated at 25 ± 1 ° C on 12 hour photoperiod. Two days after the onset of hyphae they were transferred to PDA medium plates and incubated satisfactorily under the same conditions mentioned above. After seven days the cultures obtained were identified. Fungi of the genus Trichoderma Penicillium Fusarium have been identified, and, the latter being reported as causing diseases on plant. According to these results, there is concern about the crops to be planted on site as this microorganisms can further harm the planting of some horticultural.O Brasil é um dos maiores produtores de alimentos e insumos agrícolas no mundo. Um dos grandes problemas nas plantações brasileiras é a ocorrência de doenças, que pode diminuir drasticamente a produção de uma cultura. O sucesso de uma estratégia de manejo de doenças em plantas é dependente de fatores que envolvem o conhecimento da planta, dos patógenos em potenciais e das doenças que de fato incidem sobre estas. Deste modo o trabalho teve como objetivo realizar o levantamento de fungos fitopatogenicos em uma área que será implanta uma horta voltada para a população de Dracena. A análise de solo foi baseada na metodologia de diluições seriais. Do fator de diluição 1:1000 retiraram-se alíquotas de 1,0 mL, que foram colocadas em placas de Petri contendo meio de cultura contendo 2% de ágar. As placas foram incubadas a 25±1oC em fotoperíodo 12 h. Dois dias após o aparecimento de hifas, estas foram transferidas para placas contento meio BDA e incubadas nas mesmas condições citadas anteriormente. Após sete dias as culturas obtidas foram identificadas. Foram identificados os fungos dos gêneros Trichoderma, Penicillium e Fusarium, sendo este último relatado como causador de doenças de plantas. De acordo com esses resultados, há preocupação em relação às culturas a serem plantadas no local, pois estes microrganismos podem prejudicar futuramente o plantio de algumas hortículas

Characterization of PCLO Gene in Amazonian Native American Populations

Genetic variations in PCLO have been associated with different pathologies in global literature, but there are no data regarding this gene in Native American populations. The Amazonian Native American populations have lower genetic diversity and are more different from other continental groups. We investigated 18 genetic variants in the PCLO gene in Amazonian indigenous and compared our results with the ones found in global populations, which were publicly available in the 1000 Genomes Project, gnmAD and ABraOM databases. The results demonstrated that the variants of the PCLO, especially rs17156844, rs550369696, rs61741659 and rs2877, have a significantly higher frequency in Amerindian populations in comparison with other continental populations. These data outline the singular genetic profile of the Native American population from the Brazilian Amazon region

Development of the Sm14/GLA-SE Schistosomiasis Vaccine Candidate: An Open, Non-Placebo-Controlled, Standardized-Dose Immunization Phase Ib Clinical Trial Targeting Healthy Young Women

We report the successful closure of Phase I clinical trials, comprising Phases Ia and Ib, of the vaccine candidate against human schistosomiasis: the Schistosoma mansoni 14 kDa fatty acid-binding protein (Sm14) + glucopyranosyl lipid A in squalene emulsion (GLA-SE). Shown here are the results of Phase Ib, an open, non-placebo-controlled, standardized-dose immunization trial involving 10 healthy 18–49-year-old women. Fifty micrograms of the Sm14 protein plus 10 µg GLA-SE per dose was given intramuscularly thrice at 30-day intervals. Participants were assessed clinically, biochemically, and immunologically for up to 120 days. In preambular experiments involving vaccinated pregnant female rabbits, we did not find any toxicological features in either the offspring or mothers, and the vaccine induced adaptive immunity in the animals. In women, no adverse events were observed, and vaccination induced high titers of anti-Sm14 serum IgG antibody production. Vaccination also elicited robust cytokine responses, with increased TNFα, IFNγ, and IL-2 profiles in all vaccinees on days 90 and 120. The completion of Phase I clinical trials, which were performed to the highest standards set by Good Clinical Research Practice (GCP) standards, and preclinical data in pregnant rabbits enabled the vaccine candidate to proceed to Phase II clinical trials in endemic areas

Association between the TPMT*3C (rs1142345) Polymorphism and the Risk of Death in the Treatment of Acute Lymphoblastic Leukemia in Children from the Brazilian Amazon Region

Acute lymphoblastic leukemia (ALL) is the leading cause of death from pediatric cancer worldwide. However, marked ethnic disparities are found in the treatment of childhood ALL with less effective results and higher mortality rates being obtained in populations with a high level of Native American ancestry. Genetic variations of the patient can affect resistance to ALL chemotherapy and potentially play an important role in this disparity. In the present study, we investigated the association of 16 genetic polymorphisms with the cell and metabolic pathways of the chemotherapeutic agents used in the treatment of ALL with the risk of death in treating childhood ALL in patients with a high contribution of Amerindian ancestry, coming from the Brazilian Amazon. The study included 121 patients with B-cell ALL treated with the BFM-2002 protocol. We are the first to identify the association between the TPMT gene rs1142345 polymorphism and the high risk of death in treating childhood ALL. Patients with the CC genotype had an approximately 25.5 times higher risk of dying during treatment of the disease than patients with other genotypes (p = 0.019). These results may help elucidate how the patient’s genetic characteristics contribute to the mortality disparity in populations with a high contribution of Native American ancestry. The rs1142345 variant of the TPMT gene could be used as a potential marker to early stratify patients at high risk of death in treating childhood ALL in the investigated population

Impact of pri-let-7a-1 rs10739971 for Gastric Cancer Predisposition in an Amazon Region

Gastric cancer (GC) is the fifth most common type of cancer and the fourth leading cause of cancer death. In Brazil, GC has a high incidence and mortality rates, and it is highly variable by region. The Amazon region has significant rising rates among all Brazil regions. Only very few studies have evaluated the association between genetic variants and the risk of gastric cancer in the Brazilian Amazon population. Therefore, this study aimed to investigate associations between single nucleotide polymorphisms of miRNA processing genes and the risk for GC in this population. Potentially functional single nucleotide polymorphisms from miRNA processing genes were genotyped in 159 cases and 193 healthy controls by QuantStudio Real Time PCR. According to our findings, the genotype GG of the variant rs10739971 presents a lower risk to the development of GC in comparison to the remaining genotypes (p = 0.000016; OR = 0.055; 95% CI = 0.015–0.206). This is the first study to report the association of pri-let-7a-1 rs10739971 with GC in the Brazilian Amazon population, which is a highly mixed population with a unique genetic constitution that is different from other populations that are studied in the vast majority of scientific research

Campanha de vacinação contra cinomose

Vaccination is the only way to prevent canine distemper virus, and is therefore of utmost importance, especially in the first months of life, where the animal`s immunity is low and more favorable to the disease dust contact with the virus. It is a disease that has three phases: respiratory, gastrointestinal and neurological, and can lead to death.A vacinação é a única forma de prevenção contra o vírus da cinomose, sendo, portanto, de extrema importância, principalmente nos primeiros meses de vida, onde a imunidade do animal está baixa e mais favorável a contrair a doença. É uma doença que apresenta 3 fases: respiratória, gastrointestinal e neurológica, podendo evoluir para óbito