A Crystallized Treatise on Determinants of Motivational Environment in Oil and Natural Gas Corporation Limited (ONGC)

Technologies have become stakes and those companies who can turn strategy into action at jet speed can bring seamless changes in the organization. In this context motivation has become an important HR intervention in making human resources effective. Companies realized the importance of the motivation as the contribution of employees directly relates their vision and mission statements. Demotivated workforce can cause havoc in failing to meet the organizational objectives.  It has the role to develop and intensify the desire of every member of the organization to work effectively and efficiently in their roles. After employees are hired and trained, it is important to motivate them to get the desired efforts from them, to achieve organizational objectives. Owing to the stiff competition among the Multi Nationals in retaining them has become challenge, this paper focus on motivating the workforce by introducing various HR Interventions to motivate and retain for the benefit of the organizational objectives.  For this cause a range of motivational factors were considered for a critical assessment on motivational environment in ONGC. Keywords: Motivation, technology, environment, organizational objectives, workforce DOI: 10.7176/EJBM/13-11-03 Publication date:June 30th 202

Chromosomal abnormalities and Y chromosome microdeletions in infertile men with varicocele and idiopathic infertility of South Indian origin

Various factors cause spermatogenesis arrest in men and, in a large number of cases, the underlying reason still remains unknown. Little attention is paid to determining the genetic defects of varicocele-related infertility. The objective of our present study was to investigate the chromosomal abnormalities and Y chromosome microdeletions in infertile men of South Indian origin with varicocele and idiopathic infertility. Metaphase chromosomes of 251 infertile men with varicocele and unexplained infertility were analyzed using Giemsa-Trypsin-Giemsa (GTG) banding and fluorescence in situ hybridization (FISH). The microdeletions in 6 genes and 18 sequence-tagged-sites (STS) in the Yq region were screened using polymerase chain reaction (PCR) techniques. Out of 251 infertile men, 57 (22.7%) men were with varicocele, of which 8.77% were azoospermic, 26.31% were severely oligozoospermic, 21.05% were mildly oligozoospermic, and 43.85% were oligoasthenoteratozoospermic (OAT), and 194 (77.29%), with idiopathic infertility, of which 51% were azoospermic, 13.40% were severely oligozoospermic, 19.07% were mildly oligozoospermic, and 16.4% were with OAT. Genetic defects were observed in 38 (15.13%) infertile individuals, including 14 (24.56%) men with varicocele and 24 (12.37%) men with idiopathic infertility. The frequencies of chromosomal defects in varicocele and idiopathic infertility were 19.3% and 8.76%, respectively, whereas Y chromosome microdeletions were 5.26% and 3.60%, respectively. Overall rate of incidence of chromosomal anomalies and microdeletions in 251 infertile men were 11.5% and 3.98%, respectively, indicating a very significant higher association of genetic defects with varicocele than idiopathic male infertility. Our data also demonstrate that, among infertile men with varicocele, severely oligozoospermic and OAT men with varicocele have higher incidences of genetic defects than mildly oligozoospermic and azoospermic men

De novo 7p partial trisomy characterized by subtelomeric FISH and whole-genome array in a girl with mental retardation

Chromosome rearrangements involving telomeres have been established as one of the major causes of idiopathic mental retardation/developmental delay. This case of 7p partial trisomy syndrome in a 3-year-old female child presenting with developmental delay emphasizes the clinical relevance of cytogenetic diagnosis in the better management of genetic disorders. Application of subtelomeric FISH technique revealed the presence of interstitial telomeres and led to the ascertainment of partial trisomy for the distal 7p segment localized on the telomeric end of the short arm of chromosome 19. Whole-genome cytogenetic microarray-based analysis showed a mosaic 3.5 Mb gain at Xq21.1 besides the approximately 24.5 Mb gain corresponding to 7p15.3- > pter. The possible mechanisms of origin of the chromosomal rearrangement and the clinical relevance of trisomy for the genes lying in the critical regions are discussed

Role of CYP17 and CYP19 polymorphisms in idiopathic recurrent miscarriages among South Indian women

Recent reports suggest the relevance of gene polymorphisms in predicting reproductive outcome. The present study was aimed at investigating the relationship between the 5' UTR polymorphism of CYP17, a tetranucleotide repeat and a trinucleotide deletion polymorphism in CYP19. A case-control-based study approach was used, which included 143 cases and 88 controls from the South Indian population. A PCR-sequencing based genotyping was used to ascertain the status of the individual. The A1, A2 allele frequencies of CYP17 among the cases were 72 and 28% while among the controls were 68 and 32% respectively (OR 1.21, 95% CI: 0.63-2.32). The 7/7 repeat of CYP19 was the predominant one with a distribution frequency of 55% in the cases and 58% in the controls. The deletion was observed in 39% of the cases and 36% of the controls (OR 1.14, 95% CI: 0.62-2.10). Minor genotypes when taken together represented at a higher frequency in cases than those in controls (13 against 2%, P-value: 0.006). No statistically significant association exists between the CYP17 and CYP19 trinucleotide deletion polymorphisms and the risk of idiopathic recurrent miscarriages among women from South India. However, larger repeat numbers of the tetra nucleotide (except the common 7/11 genotype) showed significant association at 95% level

Y chromosome microchimerism in female peripheral blood

Male DNA of recognized fetal origin can be detected in the maternal circulation many years after delivery. It is referred to as fetal microchimerism, and is thus a possible explanation for the existence of low-level Y chromosome mosaicisms. Employing the nested polymerase chain reaction (PCR) technique, Y-specific markers were investigated in 13 cases with abnormal sex chromosome and 31 normal women. Sex-determining region Y (SRY) sequences were detected in normal women with a male child, which reflects the existence of fetal progenitor cells in the maternal circulation. This was completely absent in normal women with a female child. Individuals with the Y chromosome showed amplification for Y-specific markers. Microchimerism of Y was noted in Turner phenotype cytogenetically investigated with marker chromosome, and in an individual with XX karyotype. False positive amplifications are possible in nested PCR reactions, but the same could also be true for routine PCR. However, in the absence of any identifiable factor that could contribute to the recurrence of spurious PCR amplifications, cases of therapeutic importance must be tested at least five times. In such situations, DNA from an additional tissue should also be used for nested PCR

Chromosome inversions and a novel chromosome insertion associated with recurrent miscarriages in South India

The aim of the present study was to investigate the contribution of chromosomal abnormalities and the frequency of a particular type of aberration in couples of South Indian origin with recurrent miscarriages. A total of 160 couples with recurrent miscarriages were analyzed using Giemsa-Trypsin-Giemsa (GTG) banding and Fluorescence in situ hybridization (FISH) wherever necessary. Chromosomal abnormalities were detected in 18 individuals representing 11.25% of the samples analyzed. Present study describes majority of the cases with chromosome inversions found to be common among the referred couples. Among the abnormal karyotypes, we report for the first time an unique case of chromosome insertion in a woman with the karyotype 46,XX,ins(12;6)(q24.2;q23q25) associated with recurrent miscarriages. The overall incidence of abnormalities and the predominance of chromosome inversions indicates to physicians that routine chromosome analysis of infertile couples of South Indian origin should be essentially considered before the planning of Intra Cytoplasmic Sperm Injection (ICSI), and also the priorities for cytogenetic screening in individual cases should be established

Mutational screening of the coding region of growth differentiation factor 9 gene in Indian women with ovarian failure

Objective: To establish the risk associated with mutations in the coding region of GDF9 gene in Indian women with ovarian failure. Design: This case-control study was designed for mutational analysis of the GDF9 coding region in a cohort of women with premature ovarian failure (n=127), primary amenorrhea (n=58), and secondary amenorrhea (n=10) compared with controls (n=220). Results: This case-control study revealed eight mutations in the GDF9 gene, including five novel mutations: c.1-8C>T, c.199A>C (p.Lys67Glu), c. 205C>T, c.646G>A (p.Val216Mat), and c.1353C>T, and three documented mutations: c.398-39C>G, c.447C>T, and c.546G>A. Missense mutation c.199A>C was present in 4 of 127 premature ovarian failure (POF) cases and 1 of 10 secondary amenorrhea cases. The c.646G>A mutation was present in two POF cases. Both missense mutations were absent in controls. Genotype distribution of c.447C>T was significantly different in POF cases than controls (χ2=5.93, P=0.05). We chose two frequent single-nucleotide polymorphisms (c.398-39C>G, c.447C>T) for haplotyping and found that the C-T haplotype was significantly higher in patients (P=0.03), whereas the C-C haplotype was representative of the control group. Conclusions: We report two rare missense mutations, c.199A>C and c.646G>A, which are associated with ovarian failure. The presence of the c.447>T mutation might indicate a higher risk for POF. Haplotype C-T was significantly associated with ovarian failure, whereas the C-C haplotype was representative of the control group

Missense mutations in the BMP15 gene are associated with ovarian failure

Premature ovarian failure (POF) is an unexplained amenorrhoea (>6 months) with raised levels of gonadotropins (FSH>40 U/L) occurring before the age of 40 years. Recent studies have elucidated the role of oocyte derived growth factors (BMP15 and GDF9) in maintenance of folliculogenesis, granulosa cell (GC) proliferation and overall fertility. Our recently published work showed presence of two rare missense variants in the GDF9 gene associated with ovarian failure. The present case-control study has been structured to establish the role of BMP15 germline status associated with ovarian failure. Sequence analysis of the coding region of the BMP15 gene was carried out in a cohort of women with POF (n=133), primary amenorrhoea (n=60), and secondary amenorrhoea (n=9) compared with control females (n=197). This study revealed a total of 18 germline variants in the coding region of BMP15 gene, including 16 novel variants. These novel variants include one intronic variant, one 3' flanking variant, one silent variant, and 13 missense variants. Eleven missense variants were present only in cases with complete absence in the control females. The remaining two missense variants viz. c.308A>G (p.Asn103Ser) and c.788_789insTCT (p.Leu263_Arg264insLeu) were present both in the cases and in the controls. The c.788_789insTCT variant was significantly higher in primary amenorrhoea cases than in the controls (Fisher's exact test, P=0.034). Three frequent variants c.-9C>G, c.308A>G, and c.852C>T were chosen for haplotyping. The haplotype G-G-C was found to be significantly associated with ovarian failure (P=0.0075). In a nutshell, the BMP15 gene is highly associated with etiology of ovarian failure

An optimized meta-heuristic clustering-based routing scheme for secured wireless sensor networks

Privacy and security present significant challenges in wireless sensor networks (WSNs). In order to enhance security, the sensor network is equipped with high throughput. While the importance of both source node (SN) and base station (BS) location privacy and security is acknowledged, recent research has predominantly focused on location privacy. Addressing this gap, the geometric zigzag bidirectional tree effectively tackles privacy and security threats at both the SN and BS locations. Future iterations of WSN are anticipated to integrate additional functionalities to meet diverse requirements in real-world applications. The absence of robust security constraints leads to an unpredictable sensor network setup. This article aims to reduce energy consumption (EC) while simultaneously enhancing network security and connectivity. To address security challenges in WSN, we propose an optimized meta-heuristic clustering-based privacy key-agreement routing technique. In the suggested system, a gateway-based network is constructed to devise a key arrangement protocol that promotes privacy during communication. The proposed routing strategy involves forming clusters of sensor nodes ((Formula presented.)), facilitating the efficient selection of cluster heads (CHs) that prioritize nodes with the least modification. This effectively addresses the EC problem. A comprehensive performance evaluation is conducted, considering improvements in energy efficiency, packet delivery ratio (PDR), throughput, end-to-end delay ((Formula presented.) delay), and EC