3 research outputs found

    Formulation and Evaluation of Mesalamine Nanosphere Tablet

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    Nanospheres are the particles having the size range between 10-200 nm in diameter. Nanospheres can be amorphous or crystalline in nature and also they have the ability to protect the drug from enzymatic and chemical degradation. For the preparation of standard calibration curve of Mesalamine with Phosphate buffer pH 6.8 and Absorbance of µg/ml solution was measured between 200-400nm by using Shimadzu 1601 UV/Vis double beam spectrophotometer. Nanosphare containing Mesalamine were prepared using nanoprecipitation method. 200 mg of polymer (Eudragit RS and L) was dissolve in 50 ml water. Drug was dissolve in 20 ml of methanol. Both solution were mixed and add 50 ml of water and stirred for half an hour. Methanol and water was evaporated under reduced pressure using rotary flash evaporator until 10 ml of solution was remaining. Than this suspension was centrifuge at 15000 rpm at 40C for half an hour. The supernatant was discarded and remaining portion was washed with distilled water. The nano-sphares was dried over night at 600C and stored in desiccators. The surface morphology (roundness, smoothness, and formation of aggregates) and particle size was studied by scanning electron microscopy (SEM). Zeta potential of the best formulation (F4) was determined by zeta potential probe model DT- 300. Mesalamimne, Dextrose and Lactose were taken in required quantities mixed and granulating agent (Starch past) was added and passed through #40 sieves, then lubricant magnesium stearate and talc was added then compressed into tablets by rotary tablet punching machine. Then film coating is done by 6% w/v solution of Cellulose acetate Phthalate in isopropyl alcohol using 2% tween-80 as plasticizer in coating pan. The weight of tablet was kept constant for all formulations. Nanosphare tablet formulation F-2 Showed maximum drug (97.75%) released and formulation F-4 showed 92.58% drug release. The In-vitro drug released study result showed that formulation F-2 96.58% drug was released after 17 hours which is highest drug release amongst all other tablet formulation. &nbsp

    Comprehensive Assessment of Transcorneal Permeation, Antimicrobial, and Antifungal Activities of Andrographolide-Loaded Nanosuspension: In vitro and In vivo Studies

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    This study aimed to comprehensively assess the transcorneal permeation, antimicrobial, and antifungal activities of a nanosuspension loaded with Andrographolide, a promising herbal compound. Through a combination of in vitro and in vivo studies, the efficacy, and potential applications of the nanosuspension in ocular drug delivery were investigated. In the in vitro phase, transcorneal permeation studies were conducted using Franz diffusion cells with excised rabbit corneas. The nanosuspension demonstrated significantly enhanced permeation compared to a control formulation, indicating its ability to effectively deliver Andrographolide through the cornea and into the ocular tissues. Additionally, the nanosuspension exhibited potent antimicrobial and antifungal activities against various ocular pathogens, as determined by agar diffusion and broth microdilution assays. Building upon the promising in vitro results, in vivo studies were performed using a rabbit model. Ocular tolerability of the nanosuspension was assessed through eye observations, with no observed signs of irritation or adverse effects. Furthermore, the nanosuspension's in vivo antimicrobial and antifungal activities were evaluated by instilling the formulation into the rabbits' eyes and monitoring conjunctival congestion and inflammation. The results demonstrated a significant reduction in conjunctival congestion, highlighting the nanosuspension's potential for combating ocular infections and inflammation. The comprehensive assessment presented in this study establishes the transcorneal permeation capability of the Andrographolide-loaded nanosuspension and its remarkable antimicrobial and antifungal activities. These findings underscore the potential of the nanosuspension as an effective ocular drug delivery system for various ocular infections and inflammatory conditions. The study contributes to the development of novel therapeutic approaches in ophthalmology, aiming to improve patient outcomes and provide alternative treatment options for ocular diseases. Keywords: Transcorneal permeation; Nanosuspension; Andrographolide; Antimicrobial activity; Antifungal activity; Ocular drug deliver

    Exploring the Potential of Ketoprofen Nanosuspension: In Vitro and In Vivo Insights into Drug Release and Bioavailability

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    Low water solubility and high permeability present formulation challenges for drugs categorized as Biopharmaceutics Classification System (BCS) Class II, resulting in reduced bioavailability. This research focuses on addressing the solubility issues of BCS Class II drugs, including Simvastatin, Ketoprofen, griseofulvin, ibuprofen, ketoconazole, and carbamazepine, which exhibit high permeability but poor solubility. A potential strategy to improve the solubility and bioavailability of these drugs is the utilization of nanosuspensions. This study investigates the application of nanosuspension technology to enhance the solubility of Ketoprofen, a BCS Class II drug. By reducing the drug's particle size within the nanosuspension, solubility is improved, leading to increased bioavailability and optimized therapeutic efficacy. The research includes in vitro and in vivo experiments to evaluate the drug release profiles and bioavailability of Ketoprofen-loaded nanosuspensions. Significant findings from this research include the demonstration of improved bioavailability and enhanced drug release properties achieved with the nanosuspension formulation. In vitro studies show increased drug dissolution rates and improved release profiles compared to conventional formulations. In vivo experiments reveal enhanced pharmacokinetic parameters and therapeutic effectiveness of Ketoprofen when administered through the nanosuspension. These results highlight the potential of nanosuspensions as an efficient drug delivery system for BCS Class II drugs, addressing their solubility limitations and improving their bioavailability. The findings contribute to the development of novel strategies in pharmacology for enhancing drug solubility and therapeutic outcomes. Overall, this research emphasizes the significance of nanosuspension technology in optimizing the delivery of BCS Class II drugs and offers valuable insights for future formulation development and therapeutic applications. Keywords: Ketoprofen, Nanosuspensions, Ultrasonication, Precipitation, Dissolution rate, Oral bioavailability
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