Desulfovibrio gigas neelaredoxin: a novel superoxide dismutase integrated in a putative oxygen sensory operon of an anaerobe

Neelaredoxin, a small non-heme blue iron protein from the sulfate-reducing bacterium Desulfovibrio gigas [Chen, L., Sharma, P., LeGall, J., Mariano, A.M., Teixeira M. and Xavier, A.V. (1994) Eur. J. Biochem. 226, 613±618] is shown to be encoded by a polycistronic unit which contains two additional open reading frames (ORF-1 and ORF-2) coding for chemotaxis-like proteins. ORF-1 has domains highly homologous with those structurally and functionally important in methyl-accepting chemotaxis proteins, including two putative transmembrane helices, potential methylation sites and the interaction domain with CheW proteins. Interestingly, ORF-2 encodes a protein having homologies with CheW proteins. Neelaredoxin is also shown to have significant superoxide dismutase activity (1200 U´mg±1), making it a novel type of iron superoxide dismutase. Analysis of genomic data shows that neelaredoxin-like putative polypeptides are present in strict anaerobic archaea, suggesting that this is a primordial superoxide dismutase. The three proteins encoded in this operon may be involved in the oxygen-sensing mechanisms of this anaerobic bacterium, indicating a possible transcriptional mechanism to sense and respond to potential stress agents

Analysis of spectral separability for detecting burned areas using Landsat-8 OLI/TIRS images under different biomes in Brazil and Portugal

Data supporting the findings of this study are available in the public domain. Landsat-8 data (https://earthexplorer.usgs.gov/, accessed on 20 April 2020). BDQueimadas vector data (https://queimadas.dgi.inpe.br/queimadas/aq30m/, accessed on 20 April 2020). ICNF burned areas vector data (https://www.icnf.pt/florestas/gfr/gfrgestaoinformacao/dfciinformacaocartgrafica, accessed on 20 April 2020).Fire is one of the natural agents with the greatest impact on the terrestrial ecosystem and plays an important ecological role in a large part of the terrestrial surface. Remote sensing is an important technique applied in mapping and monitoring changes in forest landscapes affected by fires. This study presents a spectral separability analysis for the detection of burned areas using Landsat-8 OLI/TIRS images in the context of fires that occurred in different biomes of Brazil (dry ecosystem) and Portugal (temperate forest). The research is based on a fusion of spectral indices and automatic classification algorithms scientifically proven to be effective with as little human interaction as possible. The separability index (M) and the Reed–Xiaoli automatic anomaly detection classifier (RXD) allowed the evaluation of the spectral separability and the thematic accuracy of the burned areas for the different spectral indices tested (Burn Area Index (BAI), Normalized Burn Ratio (NBR), Mid-Infrared Burn Index (MIRBI), Normalized Burn Ratio 2 (NBR2), Normalized Burned Index (NBI), and Normalized Burn Ratio Thermal (NBRT)). The analysis parameters were based on spatial dispersion with validation data, commission error (CE), omission error (OE), and the Sørensen–Dice coefficient (DC). The results indicated that the indices based exclusively on the SWIR1 and SWIR2 bands showed a high degree of separability and were more suitable for detecting burned areas, although it was observed that the characteristics of the soil affected the performance of the indices. The classification method based on bitemporal anomalous changes using the RXD anomaly proved to be effective in increasing the burned area in terms of temporal alteration and performing unsupervised detection without relying on the ground truth. On the other hand, the main limitations of RXD were observed in non-abrupt changes, which is very common in fires with low spectral signal, especially in the context of using Landsat-8 images with a 16-day revisit period. The results obtained in this work were able to provide critical information for fire mapping algorithms and for an accurate post-fire spatial estimation in dry ecosystems and temperate forests. The study presents a new comparative approach to classify burned areas in dry ecosystems and temperate forests with the least possible human interference, thus helping investigations when there is little available data on fires in addition to favoring a reduction in fieldwork and gross errors in the classification of burned areas.The article processing charge (APC) was funded by the University of Jaén through the Center for Advanced Studies on Earth Sciences, Energy and Environment CEACTEMA and the University of Minho.Research was supported by the project “Applied Remote Sensing in the Study of Hot Spots in Forests in Brazil and the Iberian Peninsula” from the Department of Cartographic Engineering and Surveying (DECart) of the Federal University of Pernambuco (UFPE/Brazil), by POIUJA-2023/2024 and CEACTEMA from University of Jaén (Spain), and RNM-282 research group from the Junta de Andalucía (Spain). This work was also supported by national funding awarded by FCT—Foundation for Science and Technology, I.P., projects UIDB/04683/2020 and UIDP/04683/2020

Experiências de vitimização de jovens adultos Portugueses

The phenomenon of victimization has been enjoying increasing attention both from the scientific community as from concerned citizens in general. In this study we examine the relationship between victimization and anti-social behavior in Portuguese community young adults (approximately 400 males and females). The group of victims and offenders differ from the groups of pure victims and controls in measures of delinquency, externalizing problems, drug abuse and domestic violence. The group of victims/offenders is a priority target for psychosocial interventions.O fenómeno da vitimização tem sido alvo de uma atenção crescente tanto por parte da comunidade científica como dos cidadãos em geral. O interesse por este problema deve-se em grande medida às dimensões desproporcionadas que este atingiu nos media e o consequente alarmismo que alastrou na sociedade sobre a capacidade que teremos para controlar, ou, de alguma maneira, mitigar os danos ou prejuízos daí decorrentes. A frequente divulgação de notícias sobre este tipo de crime certamente contribuiu para incutir uma maior consciencialização das pessoas acerca do fenómeno em si mesmo e para intensificar um sentimento de maior vulnerabilidade face a esta forma de ofensa. Os investigadores da vitimologia estão de acordo sobre a inexistência de um perfil típico da vítima. De facto, pessoas de todas as idades, raças, grupos étnicos e classes socioeconómicas são igualmente susceptíveis de serem alvo de ataques criminosos variados. Mas, por outro lado, se tivermos em consideração as estatísticas disponíveis internacionalmente é possível concluir-se que nem todos os grupos da população têm igual probabilidade de virem a ser vítimas de crime. A literatura mostra que os adolescentes mais velhos e os jovens adultos e que concomitantemente habitam em áreas urbanas com altas taxas de criminalidade, são os grupos que têm maior probabilidade de serem vítimas de crime. Além disso, a literatura mostra que aspectos relativos ao estilo de vida, local de residência e raça parecem ser importantes preditores de quem tem maior risco de ser vítima de um crime. Outro resultado bastante consistente dos estudos psicossociais atuais diz respeito à coexistência de comportamento anti-social e vitimização nos mesmos indivíduos. Estudos sobre vitimização ainda são raros em Portugal e ainda mais infrequentes são os trabalhos que procuram analisar os possíveis efeitos de variáveis de saúde mental no comportamento anti-social e na vitimização. Neste estudo iremos examinar a relação entre vitimização e comportamento anti-social em jovens adultos da comunidade Portugueses (aproximadamente 400 respondentes de ambos os sexos). Especificamente, iremos (1) examinar o grau de incidência (frequência) de vitimizações neste período da vida, (2) as características que diferenciam as vítimas das não vítimas e (3) a relação da vitimização com outros problemas da idade adulta. Dado o impacto que a experiência de vitimização tem tanto a curto como a longo prazo na vida das pessoas, o estudo destas questões é importante para um melhor entendimento das causas destes fenómenos e para o desenvolvimento de intervenções psicológicas mais eficazes nesta área

Data from Portuguese centers

© 2022 Sociedade Portuguesa de Cardiologia. Published by Elsevier España, S.L.U.INTRODUCTION: During the Covid-19 pandemic there has been a general belief that hospital admissions for non-infectious causes, especially cardiovascular disease, have fallen. OBJECTIVES: To assess the impact of the pandemic on admissions for ST-elevation myocardial infarction (STEMI) during the first pandemic wave. METHODS: We performed a multicenter retrospective analysis of consecutive patients presenting with STEMI in two Portuguese hospital centers in two sequential periods - P1 (March 1 to April 30) and P2 (May 1 to June 30). Patients' clinical data and hospital outcomes were compared between the two periods for the years 2017 to 2019 and for 2020. RESULTS: During P1 in 2020, a reduction in the number of STEMI patients was observed in comparison with previous years (26.0±4.2 vs. 16.5±4.9 cases per month; p=0.033), as well as an increase in the number of mechanical complications (0.0% vs. 3.0%; p=0.029). Percutaneous coronary interventions in the setting of failed thrombolysis were more frequent (1.9% vs. 9.1%; p=0.033). An overall trend for longer delays in key timings of STEMI care bundles was noted. Mortality was higher during P1 compared to previous years (1.9% vs. 12.1%; p=0.005). CONCLUSIONS: During the first Covid-19 wave fewer patients presented with STEMI at the catheterization laboratory for percutaneous coronary intervention. These patients presented more mechanical complications and higher mortality.proofepub_ahead_of_prin

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.info:eu-repo/semantics/publishedVersio

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.info:eu-repo/semantics/publishedVersio

Entre consumos suntuários e comuns: a posse de objetos exóticos entre alguns habitantes do Porto (séculos XVI – XVII)

O estudo da documentação referente aos doadores da Misericórdia do Porto entre os séculos XVI e XVII, através dos objetos exóticos patentes nos respectivos testamentos e inven- tários – estes últimos provenientes de uma área que se estende de Macau ao Brasil –, permite discernir uma panóplia de objetos que mudaram a cultura material dos portuenses em contato com os territórios da expansão portuguesa. Um levantamento sistemático permitiu já rastrear, até o ano de 1699, 257 doadores, dos quais se apresentarão aqui apenas alguns, referentes a benfeitores que, não obstante possuírem bens móveis nesse âmbito, não são dados como tendo estado nos territórios de expansão transoceânica. Argumentar-se-á que essa circulação de objetos não foi exclusiva das elites nobiliárquicas, nem dos grandes centros urbanos, pelo que a sua difusão atingiu maiores proporções do que aquelas que a historiografia tem admitido até agora. A cidade em observação neste estudo – o Porto dos séculos XVI e XVII – estava longe de ser das maiores da Europa nesse período, quer em dimensão territorial, quer em efetivos populacionais, embora se situasse numa região de demografia pujante, que canalizou os seus excedentes desde cedo para a emigração interna e externa – o Entre Douro e Minho. Como teremos ocasião de verificar, fidalgos e nobres possuíam bens exóticos, mas estes encontravam-se também entre mercadores e até artesãos mais desafogados. Por outro lado, nem todos os objetos provenientes dos espaços da expansão transoceânica devem ser conotados com bens de luxo.The study of the sources referring to the donors of the Misericórdia of the city of Porto during the sixteenth and seventeenth centuries has revealed the presence of numerous exotic objects in their last wills and inventories. A survey has traced 257 donors until 1699, some of them having died in an area that extends from Macao to Brazil. Only a small number of cases shall be presented here, pertaining to benefactors who, in spite of owning objects of transoceanic origin, seem to have remained in mainland Portugal. It shall be argued that the circulation of objects has not been exclusive either to the elites of the nobility or to the large urban centres, their diffusion having been on a larger scale than what has been admitted until now. The city under scrutiny in this study – Porto during the sixteenth and seventeenth centuries – was not one of the bigger cities in this period, either in what respects to size or population, although it was located in an area of flourishing demography, that channelled its surplus population early on to internal and external emigration. Fidalgos and noblemen owned exotic goods, but these were to be found among merchants and even well-to-do artisans. On the other hand, not all objects originating from the areas of transoceanic expansion should be considered as luxury goods.info:eu-repo/semantics/publishedVersio

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

O império dos mil anos e a arte do "tempo barroco": a águia bicéfala como emblema da Cristandade

The article reveals that between the mid-17th century and the mid-18th century, in the ecclesiastical world of the religious orders (Jesuits, franciscans, Carmelites, Cistercians, Augustinian, etc.) and the episcopate, there was the progressive adoption of the imperial symbol, the double-headed eagle, attribute of the Christian Empire, the germanic Holy Roman Empire emblem. However, in the religious field, this imperial eagle of the baroque time appears without the political insignia (sword, scepter and the imperial orb), adorning altars, monstrances, trumphal archs, facades of temples, doors, walls, domes, pulpits, sacred washbasins, sculptures and paintings of the Virgin and Child, liturgical robes, etc.; therefore related to the cult and the dogmas of the Catholic faith - in artistic works, the association between the double eagle and the flesh-spirit or human-divine unity, axial principle of the Catholic faith, represented by Virgin-Mother and Christ, is often indicated directly. The research has located and identified numerous remnants of the double eagle emblem in religious field in Portugal and Spain and in their conquests and dominions in America, Asia and Africa, and also in Italy, and the historiography on painful birth of the modern era in the West has never realized this phenomenon. Symbolizing absolute power, universal power, these works with sacred significance, as the ecclesiastical discourse of the time, demonstrate movement occurred in religious plane. With the aim to assert not only spiritual but also temporal power of Christ and his mystical body, the Church was impelled by the idea of restoring the "Republica Christiana" or Christendon - disrupted by conflicts of power and faith - and of introducing a Universal Apostolic Monarchy extended to all mankind: the Empire of the Last Days, the Empire of Christ in the world, the fifth Empire