12 research outputs found
Conservation and Diversity of Seed Associated Endophytes in Zea across Boundaries of Evolution, Ethnography and Ecology
Endophytes are non-pathogenic microbes living inside plants. We asked whether endophytic species were conserved in the agriculturally important plant genus Zea as it became domesticated from its wild ancestors (teosinte) to modern maize (corn) and moved from Mexico to Canada. Kernels from populations of four different teosintes and 10 different maize varieties were screened for endophytic bacteria by culturing, cloning and DNA fingerprinting using terminal restriction fragment length polymorphism (TRFLP) of 16S rDNA. Principle component analysis of TRFLP data showed that seed endophyte community composition varied in relation to plant host phylogeny. However, there was a core microbiota of endophytes that was conserved in Zea seeds across boundaries of evolution, ethnography and ecology. The majority of seed endophytes in the wild ancestor persist today in domesticated maize, though ancient selection against the hard fruitcase surrounding seeds may have altered the abundance of endophytes. Four TRFLP signals including two predicted to represent Clostridium and Paenibacillus species were conserved across all Zea genotypes, while culturing showed that Enterobacter, Methylobacteria, Pantoea and Pseudomonas species were widespread, with γ-proteobacteria being the prevalent class. Twenty-six different genera were cultured, and these were evaluated for their ability to stimulate plant growth, grow on nitrogen-free media, solubilize phosphate, sequester iron, secrete RNAse, antagonize pathogens, catabolize the precursor of ethylene, produce auxin and acetoin/butanediol. Of these traits, phosphate solubilization and production of acetoin/butanediol were the most commonly observed. An isolate from the giant Mexican landrace Mixteco, with 100% identity to Burkholderia phytofirmans, significantly promoted shoot potato biomass. GFP tagging and maize stem injection confirmed that several seed endophytes could spread systemically through the plant. One seed isolate, Enterobacter asburiae, was able to exit the root and colonize the rhizosphere. Conservation and diversity in Zea-microbe relationships are discussed in the context of ecology, crop domestication, selection and migration
A synthesis of past, current and future research for protection and management of papyrus (Cyperus papyrus L.) wetlands in Africa
Papyrus wetlands (dominated by the giant
sedge Cyperus papyrus L.) occur throughout eastern,
central and southern Africa and are important for
biodiversity, for water quality and quantity regulation
and for the livelihoods of millions of people. To draw
attention to the importance of papyrus wetlands, a
special session entitled ‘‘The ecology of livelihoods in
papyrus wetlands’’ was organized at the 9th INTECOL
Wetlands Conference in Orlando, Florida in June
2012. Papers from the session, combined with additional
contributions, were collected in a special issue
of Wetlands Ecology and Management. The current
paper reviews ecological and hydrological characteristics
of papyrus wetlands, summarizes their ecosystem
services and sustainable use, provides an
overview of papyrus research to date, and looks at
policy development for papyrus wetlands. Based on
this review, the paper provides a synthesis of research
and policy priorities for papyrus wetlands and introduces
the contributions in the special issue. Main
conclusions are that (1) there is a need for better
estimates of the area covered by papyrus wetlands.
Limited evidence suggests that the loss of papyrus
wetlands is rapid in some areas; (2) there is a need for a
better understanding and modelling of the regulating
services of papyrus wetlands to support trade-off
analysis and improve economic valuation; (3) research
on papyrus wetlands should include assessment of all
ecosystem services (provisioning, regulating, habitat,
cultural) so that trade-offs can be determined as the
basis for sustainable management strategies (‘wise
use’); (4) more research on the governance, institutional
and socio-economic aspects of papyrus wetlands
is needed to assist African governments in
dealing with the challenges of conserving wetlands in
the face of growing food security needs and climate
change. The papers in the special issue address a
number of these issues
Meta-Analyses of the Effects of Habitual Running on Indices of Health in Physically Inactive Adults
Tongue involvement in embouchure dystonia: new piloting results using real-time MRI of trumpet players
Real‐Time Magnetic Resonance Imaging
Real‐time magnetic resonance imaging (RT‐MRI) allows for imaging dynamic processes as they occur, without relying on any repetition or synchronization. This is made possible by modern MRI technology such as fast‐switching gradients and parallel imaging. It is compatible with many (but not all) MRI sequences, including spoiled gradient echo, balanced steady‐state free precession, and single‐shot rapid acquisition with relaxation enhancement. RT‐MRI has earned an important role in both diagnostic imaging and image guidance of invasive procedures. Its unique diagnostic value is prominent in areas of the body that undergo substantial and often irregular motion, such as the heart, gastrointestinal system, upper airway vocal tract, and joints. Its value in interventional procedure guidance is prominent for procedures that require multiple forms of soft‐tissue contrast, as well as flow information. In this review, we discuss the history of RT‐MRI, fundamental tradeoffs, enabling technology, established applications, and current trends