28 research outputs found
ΠΠΈΠΊΡΠΎΠ ΠΠ ΠΊΠ°ΠΊ Π²Π°ΠΆΠ½ΡΠ΅ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅cΠΊΠΈΠ΅ ΠΏΡΠ΅Π΄Π²Π΅ΡΡΠ½ΠΈΠΊΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π°ΠΊΡΡΠ΅ΡΡΠΊΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ
MicroRNAs (miRs) are the class of short nucleotide sequences (21β27 nucleotides) RNA, non-coding protein synthesis. miRs are known as effective posttranscriptional negative regulators of gene expression with specific binding sites of targeted messenger RNA (mRNA) in the cytoplasm, providing translational repression or degradation of the target miR transcript. In this review we studied the role of miRNAs in the development of a physiological pregnancy and obstetric complications. The placenta is a unique organ which provides modulation of the immune system of the maternal organism during pregnancy including miRs which determine immunological tolerance of the body to the tissues of the fetus. Thus the Β«placentalΒ» miRs in maternal circulation may be the potential biomarker revealed at various obstetric pathology on the early stages before clinical manifestation of the diseases.ΠΠΈΠΊΡΠΎΠ ΠΠ (ΠΌΠΊΠΠ) β ΠΊΠ»Π°ΡΡ ΠΊΠΎΡΠΎΡΠΊΠΈΡ
Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ
ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ (21β27 Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ²) Π ΠΠ, Π½Π΅ ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ
Π² ΡΠΈΠ½ΡΠ΅Π·Π΅ Π±Π΅Π»ΠΊΠ°. ΠΌΠΊΠ ΠΠ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡ ΠΊΠ°ΠΊ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠ΅ ΠΏΠΎΡΡΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΡΠ΅ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΡΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ², ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠΈ- Π΅ΡΡ ΡΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΡΠ°ΡΡΠΊΠ°ΠΌΠΈ ΠΌΠ°ΡΡΠΈΡΠ½ΡΡ
Π ΠΠ (ΠΌΠ ΠΠ) Π² ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ΅, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΠ΅ ΡΠ΅ΠΏΡΠ΅ΡΡΠΈΡ ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ ΠΈΠ»ΠΈ Π΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² ΠΌΠΈΡΠ΅Π½Π΅ΠΉ ΠΌΠ ΠΠ. ΠΠΎΠΊΠ°Π·Π°Π½Π° ΡΠΎΠ»Ρ ΠΌΠΊΠ ΠΠ Π² ΡΠ°Π·Π²ΠΈΡΠΈΠΈ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠΈ ΠΈ Π΅Π΅ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ, ΡΠ΄Π΅Π»Π΅Π½ΠΎ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΠΏΡΠΎΡΠΈΠ»ΡΠΌ ΠΌΠΊΠ ΠΠ Π½Π° ΡΠ°Π·Π½ΡΡ
ΡΡΠΎΠΊΠ°Ρ
ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠΈ, ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎ ΠΏΡΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠΎΠ»Π΅ΡΠ°Π½ΡΠ½ΠΎΡΡΠΈ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ° ΠΌΠ°ΡΠ΅ΡΠΈ ΠΊ ΡΠΊΠ°Π½ΡΠΌ ΠΏΠ»ΠΎΠ΄Π° Π²ΠΎ Π²ΡΠ΅ΠΌΡ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠΈ. Π’Π°ΠΊΠΆΠ΅ ΠΎΡΠΌΠ΅ΡΠ΅Π½Ρ ΠΌΠΊΠ ΠΠ, Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Ρ ΠΈΠΌΠΌΡΠ½ΠΎΡΡΠΏΡΠ΅ΡΡΠΈΠ΅ΠΉ, ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ ΠΏΡΠΎΡΠΈΠ»Ρ ΠΌΠΊΠ ΠΠ, ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Ρ ΠΏΡΠ΅ΡΠΊΠ»Π°ΠΌΠΏΡΠΈΠ΅ΠΉ. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ»Π°ΡΠ΅Π½ΡΠ°ΡΠ½ΡΡ
ΠΌΠΊΠ ΠΠ Π² ΠΌΠ°ΡΠ΅ΡΠΈΠ½ΡΠΊΠΎΠΌ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ΅ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄ΠΎΠ²Π°Π½ΠΎ ΠΈΡΠΏΠΎΠ»Ρ- Π·ΠΎΠ²Π°ΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠ°Π½Π½Π΅Π³ΠΎ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅ΡΠ° Π·Π½Π°ΡΠΈΠΌΠΎΠΉ Π°ΠΊΡΡΠ΅ΡΡΠΊΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Π΄ΠΎ Π½Π°ΡΡΡΠΏΠ»Π΅Π½ΠΈΡ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΌΠ°Π½ΠΈΡΠ΅ΡΡΠ°ΡΠΈΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ
Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised study
Background. Personalised medicine is an avenue to create technologies for individual prognosis of the disease onset and development. The identification of individual gene haplotypes is prerequisite to detecting predispositions to multifactorial diseases. The level of serum 8-oxoguanine is an indicator of genotoxic stress underlying many pathologies.Objectives. A study of associations of mmp12 geneβs polymorphic variant rs652438 and the nature of genome oxidative damage in bronchial asthma.Methods. Genotyping of polymorphic variant rs652438 of gene mmp12 was performed using TaqMan-probe real-time PCR assays. The gene variant association with disease was assessed by odds ratio. The degree of DNA oxidative damage was estimated by 8-oxoguanine serum concentrations determined in monoclonal antibody-based enzyme immunoassays. The StatPro software package with StatTools (Palisade Corporation, USA) was used for statistical data processing.Results. The haplotype and allele frequencies were established for polymorphic locus rs652438 of the mmp12 gene in the control and bronchial asthma cohorts. Heterozygotes were shown to differ significantly; the estimate was 2.3-fold higher in the control vs. bronchial asthma (BA) cohort (p < 0.05). The AA and GG haplotype frequencies did not differ significantly. The minor allele G odds ratio (OR = 0.362, CI 95% 0.134β0.975) suggests its protective effect. This may be associated with a lowering activity of the encoded macrophage metalloelastase enzyme, which results in a poorer extracellular matrix destruction in the bronchial tree. The baseline 8-oxoG levels in the control and BA samples were 6.4 and 9.4 ng/mL, respectively (U = 25, Ucut-off = 23; p >0.05). An in vitro electromagnetic exposure of varying frequency leads to a significant oxidative genomic damage in both cohorts and an earlier reparative depletion in bronchial asthma vs. control.Conclusion. A protective effect of minor allele G against pathology has been demonstrated. Adaptations to oxidative genomic stress in bronchial asthma manifest by an impaired resistance to in vitro high-intensity electromagnetic exposures
Weather risk factors for stroke in the Central Region of Russia
The weather risk factors of stroke were studied in the Central Region of Russia. Case histories of patients admitted to Yaroslavl Clinical Hospital Eight with a diagnosis of stroke from October 2002 to December 2006 were analyzed. Among 3243 patients, there were 1607 men and 1636 women (mean age 62.45Β±12.19years. Ischemic and hemorrhagic strokes were identified in 61.1 and 19.0% of cases, respectively. The risk factors of stroke were found to be essential hypertension (70.4%), cerebral atherosclerosis (35.2%), and coronary heart disease (17.4%). A significant role is played in the occurrence of the disease by weather factors, such as wind speed (contribution factor, 32.1%), average daily air temperature (contribution factor, 17.9%), and atmospheric pressure (contribution factor, 17.1%)
Efficacy and tolerability of Potentilla tincture in complex therapy of patients with knee osteoarthritis
Objective. To assess efficacy and safety of two-month therapy with Potentilla tincturecombined with diclofenac in pts with osteoarthritis (OA) in comparison with diclofenac monotherapy (control group). Material and methods. 60 pts with OA aged 50-75 years were randomly assigned into two groups 30 pts in each. Knee OA was diagnosed according to ACR criteria (1991). Pain at movement, WOMAC index (on VAS), efficacy assessment by physician and pt and diclofenac requirement were used as outcome measures. 1 tea spoon of Potentilla tincture diluted in 1/3 of water glass was given twice a day before meal as well as diclofenac 100 mg/ day during 2 months. Results. Combined therapy provided prominent decrease of pain which exceeded improvement in control group. Significant decrease of summated WOMAC index was achieved only in the main group. Conclusion. Combination of Potentilla tincture and diclofenac was significantly more effective than treatment with diclofenac alone in OA. Combined therapy provided more pronounced analgesic and anti-inflammatory effect than diclofenac monotherapy
Preeclampsia: The Interplay between Oxygen-Sensitive miRNAs and Erythropoietin
Changes in the oxygen partial pressure caused by a violation of uteroplacental perfusion are considered a powerful inducer of a cascade of reactions leading to the clinical manifestation of preeclampsia (PE). At the same time, the induction of oxygen-dependent molecule expression, in particular, miRNA and erythropoietin, is modulated. Therefore, the focus of our study was aimed at estimating the miRNA expression profile of placental tissue and blood plasma in pregnant women with preeclampsia using deep sequencing and quantitative RT-PCR, as well as determining the concentration of erythropoietin. The expression of miR-27b-3p, miR-92b-3p, miR-125b-5p, miR-181a-5p, and miR-186-5p, as regulated by hypoxia/reoxygenation, was significantly increased in blood plasma during early-onset preeclampsia. The possibility of detecting early PE according to the logistic regression model (miR-92b-3p, miR-125b-5p, and miR-181a-5p (AUC = 0.91)) was evaluated. Furthermore, the erythropoietin level, which is regulated by miR-125b-5p, was significantly increased. According to PANTHER14.1, the participation of these miRNAs in the regulation of pathways, such as the hypoxia’s response via HIF activation, oxidative stress response, angiogenesis, and the VEGF signaling pathway, were determined