1 research outputs found
Osteoinduction in human fat derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli
Bioactive recombinant human bone
morphogenetic protein-2 (rhBMP-2) was obtained
using Escherichia coli pET-25b expression system:
55 mg purified rhBMP-2 were achieved per g cell dry
wt, with up to 95% purity. In murine C2C12 cell line,
rhBMP-2 induced an increase in the transcription of
Smads and of osteogenic markers Runx2/Cbfa1 and
Osterix, measured by semi-quantitative RT-PCR.
Bioassays performed in human fat-derived stem cells
showed an increased activity of the early osteogenic
marker, alkaline phosphatase, and the absence of
cytotoxicity