Enhanced production of cellulases by various fungal cultures in solid state fermentation of cassava waste

Cellulases are a group of hydrolytic enzymes capable of degrading cellulose to the smaller glucose units. These enzymes are produced by fungi and bacteria. The solid waste of sago industry using cassava tubers was fermented by Aspergillus niger, Aspergillus terreus and Rhizopus stolonifer in solid state fermentation. The cassava waste contained dry wt of 13.4% cellulose and 2.9% protein by dry weight. The highest cellulase activity was observed on the 10th day in R. stolonifer mediated fermentation. R. stolonifer was more efficient in bioconverting cassava waste into fungal protein (9%) compared to A. niger and A. terreus

Screening of winery and olive mill wastes for lignocellulolytic enzyme production from Aspergillus species by solid-state fermentation

Wastes from olive oil and wine industries (as exhausted grape marc, vineshoot trimmings, two-phase olive mill waste, vinasses, and olive mill wastewater) were evaluated for lignocellulolytic enzyme production (as endocellulases, endoxylanases, and feruloyl esterases) by solid-state fermentation (SSF) with Aspergillus niger, Aspergillus ibericus, and Aspergillus uvarum. To study the effect of different solid medium composition and time in enzyme production, a PlackettBurman experimental design was used. Variables that had a higher positive effect in lignocellulolytic enzyme production were urea, time, and exhausted grape marc. The maximum values of enzymatic activity per unit of substrate dry mass were found with A. niger for feruloyl esterase. Enzymatic extracts from SSF with A. niger achieved maximum feruloyl esterase activity (89.53 U/g) and endoxylanase activity (3.06 U/g) and with A. uvarum for endocellulase activity (6.77 U/g). The enzyme cocktails obtained in the SSF extracts may have applications in biorefinery industries.Jose Manuel Salgado is grateful for the postdoctoral fellowship (EX-2010-0402) of the Education Ministry of Spanish Government. Luis Abrunhosa was supported by the grant SFRH/BPD/43922/2008 from Fundacao para a Ciencia e Tecnologia-FCT, Portugal

Marker Assisted Selection for Leaf Blast Disease resistance and other yield traits in rice (Oryza sativa L.) using RAPD Markers

A segregating population of 220 F2 individuals was developed by raising the selfed F1 seeds of a single hybrid plant, White Ponnix Moroberekan. Two hundred and twenty F3 families were phenotyped for blast resistance under artificial condition. Bulksegregantanalysis resulted in the identification of 12 leaf blast resistant phenotype specific RAPD markers. Mapping survey wasdone with seven RAPD primers which co-segregated in BSA. Twelve RAPD markers were scored in the F2 mapping population.Out of 12 marker loci, 11 marker loci (91.97 per cent) fitted into the expected segregation ratio of 3:1 based on χ2- test at 0.05per cent probability value. One way ANOVA was performed to identify the marker phenotype association and this resulted in theidentification of seven RAPD markers linked to three traits viz., leaf blast resistance (OPBB 5258, OPBB 5194, OPAL 16940 andOPBD 12680), five markers for GPT and one marker for grain yield per plant

Surface Modification of Coir Fibers for Extended Hydrophobicity and Antimicrobial Property for Possible Geotextile Application

Experimental investigations are conducted to study the effectiveness of protective coating with cashew nut shell liquid (CNSL) on coir. CNSL solution has been applied on suitably pretreated coir yarns and coating concentrations are optimized to reduce moisture absorption and microbial growth. Two common cellulose and lignin degrading fungi (Aspergillus niger and Rhizopus stolonifer) have been taken as test organisms. Qualitative and quantitative analyses are carried out to measure fungal growth on untreated and treated fibers. The study shows that treated fibers inhibit the development of fugal growth on fiber surface by 95%. The reduced moisture absorption and improved hydrophobicity of the treated yarns are also reported. Increase in tensile strength up to 17% is noticed. The better properties achieved are explained on the basis of selection of the coating methodology and also the interfacial features of the coatings with the coir fiber. The results indicate the possibility of future application of such treated fibers in geotextiles having better performance compared to the untreated counterparts

Optimization of fermentation conditions for cellulases production by Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from Indian hot spring

The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55°C, respectively. Maximum cellulases production by both the isolates was detected after 60 h incubation period using wheat and rice straw. The combination of inorganic and organic nitrogen source was suitable for cellulases production. Overall, FPase production was much higher than CMCase production by both of the strains. Between the two thermophiles, the cellulolytic activity was more in B.licheniformis MVS1 than Bacillus sp. MVS3 in varying environmental and nutritional conditions