Canine Wharton's Jelly Derived Mesenchymal Stem Cells Isolation

AbstractMesenchymal stem cells (MSCs) represent an attractive source of cells for cell therapy in veterinary medicine. Recent interest in stem cell biology and its therapeutic potential has led to search for accessible new sources of stem cells. Wharton‘s Jelly-derived mesenchymal stem cells are multipotent with specific potential to differentiate into multiple lineages.The aim of our study was the isolation, characterization and multilineage differentiation of Wharton‘s Jelly derived mesenchymal stem cells obtained from canine umbilical cord following Caesarean section.Our data confirmed that the isolated and cultivated mesenchymal stem cells have multipotent based on specific surface antigen expressing and differentiation capacity

Mastitis Acuta in a Pure Breed Cane Corso female. A Case Report

Data regarding bitch mastitis is fairly scarce compared to the literature regarding ruminant mastitis. Neglecting clinical and subclinical cases of mastitis can be life threatening for both dam and puppies. The aim of this report is to present a case of Mastitis acuta complicated with a case of neonatal septicaemia in a Cane Corso (Canis lupus familiaris L.) pure breed female. Laboratory assays showed a milk pH value of 7.5, milk cytology revealed the presence of segmented neutrophils, while foamy cells, phagocytosis and highly pathogenic bacteria (i.e. Escherichia coli and Pseudomonas aeruginosa) were isolated from milk and puppy. To the author's knowledge, this is the first case of bitch mastitis linked to neonatal septicaemia with mixed E. coli and P. aeruginosa infection