Differences in the Pathways of Proteins Unfolding Induced by Urea and Guanidine Hydrochloride: Molten Globule State and Aggregates

It was shown that at low concentrations guanidine hydrochloride (GdnHCl) can cause aggregation of proteins in partially folded state and that fluorescent dye 1-anilinonaphthalene-8-sulfonic acid (ANS) binds with these aggregates rather than with hydrophobic clusters on the surface of protein in molten globule state. That is why the increase in ANS fluorescence intensity is often recorded in the pathway of protein denaturation by GdnHCl, but not by urea. So what was previously believed to be the molten globule state in the pathway of protein denaturation by GdnHCl, in reality, for some proteins represents the aggregates of partially folded molecules

Soils and Productivity

This chapter explores the predominant land-based production systems in Ireland, specifically describing grassland, arable and forestry production. Grass is the most important agricultural crop in Ireland representing the main feed source for the livestock sector. Arable production represents a relatively small area, largely due to excess soil moisture conditions that are typical of many Irish soils. Despite favourable conditions for forestry in Ireland, the level of forest cover is low by European standards and has historically been associated with sub-marginal and marginal agricultural land. The importance of soil structure in the provision of nutrients, water and air is emphasised along with best management practices in relation to the management cycle. The role of soil texture is outlined, as is the agroclimate and soil interaction in Irish soils. The agricultural drainage conditions of Irish soils fall into the temperate zone, where the main role of drainage is the removal of excess water in the root zone of crops from surplus rainfall. This follows with a historical account of land drainage schemes in Ireland. Finally, the issues of the main production systems on poorly drained soils and the cultural practices to maintain trafficability are described. Improving the overall trafficability of any soil first requires the identification of factors causing either excessive moisture or poor soil structure. Correctly ascertaining these issues allows implementation of appropriate preventative and remediation measures, and should be the initial step in any management strategy