3 research outputs found
Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π³Π΅Π½ΠΎΠ² ompA, ct135 ΠΈ tarP ΡΡΠ°ΠΌΠΌΠΎΠ² C. trachomatis, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ ΠΎΡ Π±ΠΎΠ»ΡΠ½ΡΡ ΡΡΠΎΠ³Π΅Π½ΠΈΡΠ°Π»ΡΠ½ΠΎΠΉ Ρ Π»Π°ΠΌΠΈΠ΄ΠΈΠΈΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ
The article contains results of the molecular and genetic research of genes ompA, ct135 and tarp c. trachomatis in 50 samples of clinical material containing DNA c. trachomatis from patients (women and men) from the Moscow region with the diagnosed urogenital chlamydial infection (UGCI) confirmed by PCR method. The sequencing of the gene ompA allowed to establish, that the prevailing serovar c. trachomatis in the examined selection of UGCI patients was serovar Π (48,0%); other serovars were less often (G β 18,0%, D β 14,0%; F and J β by 8%, Π β 4%). The authors revealed authentic associations of the frequency of spreading of Π serovar with non-complicated clinical course of UGCI and the association of the frequency of spreading concerning rare serovars c. trachomatis (G, D, J) β with complications of UGCI in women. Significant mutations of the gene ompΠ in rare c. trachomatis serovars, Π°s well as significant mutations of genes ct135 and tarΡ were revealed more often is samples from women with PID. The obtained data are sufficient to make a conclusion about the possible existing relation between peculiarities of c. trachomatis genotype and the character of the clinical course of the urogenital clamidiosis.ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π³Π΅Π½ΠΎΠ² ompA, ct135 ΠΈ tarP c. trachomatis Π² 50 ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°, ΡΠΎΠ΄Π΅ΡΠΆΠ°Π²ΡΠΈΡ
ΠΠΠ c. trachomatis ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΎΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² (ΠΆΠ΅Π½ΡΠΈΠ½ ΠΈ ΠΌΡΠΆΡΠΈΠ½) ΠΈΠ· ΠΠΎΡΠΊΠΎΠ²ΡΠΊΠΎΠ³ΠΎ ΡΠ΅Π³ΠΈΠΎΠ½Π° Ρ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π Π΄ΠΈΠ°Π³Π½ΠΎΠ·ΠΎΠΌ ΡΡΠΎΠ³Π΅Π½ΠΈΡΠ°Π»ΡΠ½ΠΎΠΉ Ρ
Π»Π°ΠΌΠΈΠ΄ΠΈΠΉΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ (Π£ΠΠ₯Π). Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π³Π΅Π½Π° ompA ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠΌ ΡΠ΅ΡΠΎΠ²Π°ΡΠΎΠΌ c. trachomatis Π² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΠΎΠΉ Π²ΡΠ±ΠΎΡΠΊΠ΅ Π±ΠΎΠ»ΡΠ½ΡΡ
Π£ΠΠ₯Π ΡΠ²Π»ΡΠ»ΡΡ ΡΠ΅ΡΠΎΠ²Π°Ρ Π (48%); Π΄ΡΡΠ³ΠΈΠ΅ ΡΠ΅ΡΠΎΠ²Π°ΡΡ Π²ΡΡΡΠ΅ΡΠ°Π»ΠΈΡΡ Ρ ΠΌΠ΅Π½ΡΡΠ΅ΠΉ ΡΠ°ΡΡΠΎΡΠΎΠΉ (G β 18%, D β 14%; F ΠΈ J β ΠΏΠΎ 8%, Π β 4%). ΠΡΡΠ²Π»Π΅Π½Ρ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΡΠ΅ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ ΡΠ°ΡΡΠΎΡΡ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½ΠΈΡ ΡΠ΅ΡΠΎΠ²Π°ΡΠ° Π Ρ Π½Π΅ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½Π½ΡΠΌ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ΠΌ Π£ΠΠ₯Π ΠΈ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ ΡΠ°ΡΡΠΎΡΡ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½ΠΈΡ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ΅Π΄ΠΊΠΎ Π²ΡΡΡΠ΅ΡΠ°ΡΡΠΈΡ
ΡΡ ΡΠ΅ΡΠΎΠ²Π°ΡΠΎΠ² c. trachomatis (G, D, J) β Ρ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΡΠΌΠΈ Π£ΠΠ₯Π Ρ ΠΆΠ΅Π½ΡΠΈΠ½. ΠΠ½Π°ΡΠΈΠΌΡΠ΅ ΠΌΡΡΠ°ΡΠΈΠΈ Π³Π΅Π½Π° ompΠ Π² ΡΠ΅Π΄ΠΊΠΎ Π²ΡΡΡΠ΅ΡΠ°ΡΡΠΈΡ
ΡΡ ΡΠ΅ΡΠΎΠ²Π°ΡΠ°Ρ
c. trachomatis, Π° ΡΠ°ΠΊΠΆΠ΅ Π·Π½Π°ΡΠΈΠΌΡΠ΅ ΠΌΡΡΠ°ΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² ct135 ΠΈ tarP ΡΠ°ΡΠ΅ ΠΎΠ±Π½Π°ΡΡΠΆΠΈΠ²Π°Π»ΠΈΡΡ Π² ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΎΡ ΠΆΠ΅Π½ΡΠΈΠ½ Ρ ΠΠΠΠΠ’. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΡΠ΄Π΅Π»Π°ΡΡ Π·Π°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΎ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠΌ ΡΡΡΠ΅ΡΡΠ²ΠΎΠ²Π°Π½ΠΈΠΈ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΡΠΌΠΈ Π³Π΅Π½ΠΎΡΠΈΠΏΠ° c. trachomatis ΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΎΠΌ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΡΡΠΎΠ³Π΅Π½ΠΈΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Ρ
Π»Π°ΠΌΠΈΠ΄ΠΈΠΎΠ·Π°
ΠΠΌΠΌΡΠ½Π½ΡΠ΅ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΡ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ ΡΡΠΎΠ³Π΅Π½ΠΈΡΠ°Π»ΡΠ½ΠΎΠΉ Ρ Π»Π°ΠΌΠΈΠ΄ΠΈΠΉΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ, ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΠΈΡ ΠΊ Π½Π°ΡΡΡΠ΅Π½ΠΈΡΠΌ ΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠΉ ΡΡΠ½ΠΊΡΠΈΠΈ
Goal: determination of immune predictors of the development of complications of the urogenital chlamydial infection (UGCI) in women and further disorder of the reproductive function (infertility) due to the past infection. ΠΠ°terials and methods. 80 women have been examined: 40 patients with uGCI (20 β with UGCI of lower sections of the urogenital tract and 20 β with pelvic UGCI), 20 patients with secondary (tubal) infertility with the previous UGCI in the anamnesis and 20 clinically healthy women. The qualitative and quantitative study of the spectrum of cytokines (IL-2, -4, -6, -8, -10, INF-Ξ³, TNF Ξ± and GM-CSF) with the use of the Ρ
ΠΠΠ multiplex technology in blood and the cervical secretion. Results. The research has determined the spectrum of cytokines with a change in their concentration having an effect on the outcome of UGCI and the development of complications causing disorders of the reproductive function in women: these are TNF-Ξ±, IFN-Ξ³, IL-2 inflammatory cytokines Π°s well as the IL-10 inflammatory cytokine.Π¦Π΅Π»Ρ. ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΠΈΠΌΠΌΡΠ½Π½ΡΡ
ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΠΎΠ² ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ ΡΡΠΎΠ³Π΅Π½ΠΈΡΠ°Π»ΡΠ½ΠΎΠΉ Ρ
Π»Π°ΠΌΠΈΠ΄ΠΈΠΉΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ (Π£ΠΠ₯Π) Ρ ΠΆΠ΅Π½ΡΠΈΠ½ ΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅Π³ΠΎ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠΉ ΡΡΠ½ΠΊΡΠΈΠΈ (Π±Π΅ΡΠΏΠ»ΠΎΠ΄ΠΈΡ) Π²ΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠ΅ ΠΏΠ΅ΡΠ΅Π½Π΅ΡΠ΅Π½Π½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ 80 ΠΆΠ΅Π½ΡΠΈΠ½: 40 Π±ΠΎΠ»ΡΠ½ΡΡ
Π£ΠΠ₯Π (20 β Ρ Π£ΠΠ₯Π Π½ΠΈΠΆΠ½ΠΈΡ
ΠΎΡΠ΄Π΅Π»ΠΎΠ² ΡΡΠΎΠ³Π΅Π½ΠΈΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° ΠΈ 20 β Ρ ΡΠΡ
ΠΈ ΠΎΡΠ³Π°Π½ΠΎΠ² ΠΌΠ°Π»ΠΎΠ³ΠΎ ΡΠ°Π·Π°), 20 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ Ρ Π²ΡΠΎΡΠΈΡΠ½ΡΠΌ (ΡΡΡΠ±Π½ΡΠΌ) Π±Π΅ΡΠΏΠ»ΠΎΠ΄ΠΈΠ΅ΠΌ Ρ ΠΏΠ΅ΡΠ΅Π½Π΅ΡΠ΅Π½Π½ΠΎΠΉ Π£ΠΠ₯Π Π² Π°Π½Π°ΠΌΠ½Π΅Π·Π΅ ΠΈ 20 ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
ΠΆΠ΅Π½ΡΠΈΠ½. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΡΠ»ΡΡΠΈΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠΉ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ Ρ
ΠΠΠ Π² ΠΊΡΠΎΠ²ΠΈ ΠΈ ΡΠ΅ΡΠ²ΠΈΠΊΠ°Π»ΡΠ½ΠΎΠΌ ΡΠ΅ΠΊΡΠ΅ΡΠ΅ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΏΠ΅ΠΊΡΡΠ° ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (IL-2, -4, -6, -8, -10, INF-Ξ³, TNF-Ξ± ΠΈ GM-CSF). Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π Ρ
ΠΎΠ΄Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ» ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ ΡΠΏΠ΅ΠΊΡΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ², ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ ΠΊΠΎΡΠΎΡΡΡ
ΠΌΠΎΠΆΠ΅Ρ Π²Π»ΠΈΡΡΡ Π½Π° ΠΈΡΡ
ΠΎΠ΄ Π£ΠΠ₯Π ΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ, ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΠΈΡ
ΠΊ Π½Π°ΡΡΡΠ΅Π½ΠΈΡΠΌ ΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠΉ ΡΡΠ½ΠΊΡΠΈΠΈ Ρ ΠΆΠ΅Π½ΡΠΈΠ½: ΡΡΠΎ ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΠΈΡΠΎΠΊΠΈΠ½Ρ TNF-Ξ±, IFN-Ξ³, IL-2, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠΈΡΠΎΠΊΠΈΠ½ IL-10