Alterações químicas durante o desenvolvimento e maturação de manga Tommy Atkins cultivada no Vale do São Francisco.

O objetivo deste trabalho foi caracterizar alterações químicas relacionadas à qualidade da manga Tommy Atkins, durante o desenvolvimento e maturação, nas condições de cultivo do Vale do São Francisco. A manga Tommy Atkins madura apresentou o máximo teor de sólidos solúveis totais (SST), porém os teores de vitamina C e de carotenóides totais da polpa foram inferiores aos dos frutos em estádio inicial de maturação. As mudanças na acidez total titulável e nos teores de SST e carotenóides totais da polpa foram indicativas da evolução da maturação

Estratégias de sucessão trigo/aveia preta-soja para sistemas de produção de grãos no Noroeste do Rio Grande do Sul.

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Estratégias de sucessão trigo/aveia preta-soja para sistemas de produção de grãos no Planalto Médio do Rio Grande do Sul.

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Estratégias de sucessão trigo/cevada/aveia preta/soja para sistemas de produção de grãos no Centro-Sul do Paraná.

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Memória da Oficina Ranqueamento de Indicadores de Serviços Ambientais.

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A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium.

Proline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases