Near Infrared Spectroscopy to Assess Feeding Value and Antinutritional Compounds in Legume Species

There is an increasing demand for information on the quality characteristics and chemical composition of forages in order to meet the demands of dietary specifications for feeding animals. Near Infrared (NIR) spectroscopy provides a tool for rapid and non-destructive analysis in agronomic and breeding programs of a number of chemical components of forages and grains. NIR spectroscopy in particular has the advantage of being able to simultaneously evaluate the samples for a number of qualitative traits of whole plants and seeds. In two experiments here presented, NIR Spectroscopy was used to predict: i) qualitative characteristics of field pea seeds and, as regards secondary metabolites responsible of detrimental or beneficial effects on animal nutrition ii) condensed tannins in legume forages, based on calibration sets of samples previously chemically analysed

Proanthocyanidins from \u3ci\u3eHedysarum, Lotus\u3c/i\u3e and \u3ci\u3eOnobrychis\u3c/i\u3e spp. Growing in Sardinia and Sicily and Their Antioxidant Activity

Proanthocyanidins (PA), or condensed tannins, are a class of natural polyphenolic compounds, occurring in numerous plant species, including a number of economically significant forage legumes. These compounds are polymers of flavan-3-ols, and typically contain from 2 to 20 units. Their biological significance is still being debated and, in recent years, a great deal of attention has been focused on their role in ruminant nutrition. Evidence has indicated that PA, in a moderate concentration (0.5-5% DM), may have considerable importance in protecting dietary proteins against microbial degradation in the rumen, and in preventing bloat. The antioxidant activity (AA) is also an important feature for animal well-being (Barry & McNabb, 1999). In order to study the PA content related to the antioxidant activity, samples of Hedysarum, Lotus and Onobrychis spp. from Mediterranean environments have been considered and investigated

Proanthocyanidins from \u3cem\u3eHedysarum, Lotus\u3c/em\u3e and \u3cem\u3eOnobrychis\u3c/em\u3e spp. Growing in Sardinia and Sicily and Their Antioxidant Activity

Proanthocyanidins (PA), or condensed tannins, are a class of natural polyphenolic compounds, occurring in numerous plant species, including a number of economically significant forage legumes. These compounds are polymers of flavan-3-ols, and typically contain from 2 to 20 units. Their biological significance is still being debated and, in recent years, a great deal of attention has been focused on their role in ruminant nutrition. Evidence has indicated that PA, in a moderate concentration (0.5-5% DM), may have considerable importance in protecting dietary proteins against microbial degradation in the rumen, and in preventing bloat. The antioxidant activity (AA) is also an important feature for animal well-being (Barry & McNabb, 1999). In order to study the PA content related to the antioxidant activity, samples of Hedysarum, Lotus and Onobrychis spp. from Mediterranean environments have been considered and investigated

Combined effects of microenvironment and land use on C fluxes in a Mediterranean agro-silvopastoral system

none7noAppropriate agroforestry practices might contribute to carbon sequestration and cope with climate change by modulating ecosystem services. It is known that land use change might affect soil-atmosphere carbon dioxide (CO2) effluxes of agro-silvopastoral systems. However, little information is available at single microenvironment level. Across four years, fortnightly measurements of soil respiration were carried out at different microenvironment (beneath tree cover vs open areas) and land use (native understorey vs its conversion into improved pasture) within a high-density evergreen cork oak forest of Sardinia (Italy). We also monitored aboveground dry matter yields and soil carbon stocks. Measurements revealed that the two investigated microenvironments widely differed for the amounts of photosynthetically active radiation and microclimatic traits such as soil water content, air humidity, soil and air temperature. High seasonal and inter-annual variability in soil respiration rates was recorded (range 0.3–12.6 CO2 μmol m−2 s-1) and the peak values were reached in the summer of the third year in the improved pasture beneath tree cover. The conversion of the native understorey into improved pasture beneath cork oak increased significantly the annual cumulative soil respiration for three consecutive years, reaching values of about 71, 36 and 100 t CO2 ha−1 yr−1, which were from 38 to 88 % higher than the remaining treatments. On average, heterotrophic component represented from 68–76% of soil respiration. An extreme drought event, which was emblematic of a climate change context, was experienced in the second year. It countered the increase in the heterotrophic component of soil respiration and minimized up to 20-fold the forage on offer from pasture swards. Based on measured CO2 effluxes, the study demonstrated that the effects of the land use change at the microenvironment beneath tree cover were substantially unbalanced in terms of soil organic carbon stocks. Therefore, results suggest avoiding the soil mechanical disturbance beneath cork oak in the investigated ecosystem to reduce anthropogenic carbon fluxes to the atmosphere.mixedSanna F.; Campesi G.; Deligios P.; Ledda L.; Piluzza G.; Sulas L.; Re G.A.Sanna, F.; Campesi, G.; Deligios, P.; Ledda, L.; Piluzza, G.; Sulas, L.; Re, G. A

Inoculation and N fertilization affect the dry matter, N fixation, and bioactive compounds in Sulla leaves

Sulla (Sulla coronaria [L.] Medik), a Mediterranean short-lived legume with tolerance to drought-prone environments, requires inoculation outside its natural habitat. Its leaves are appreciated for the bromatological composition and content of bioactive compounds. However, no information is available regarding the distinct effects of inoculation and nitrogen (N) applications on leaf dry matter (DM), fixed N, and bioactive compounds. Sulla leaves were sampled from the vegetative stage to seed set in Sardinia (Italy) during 2013–2014 and leaf DM, N content, and fixed N were determined. Compared to the best performing inoculated treatments, DM yield and fixed N values of the control only represented 8% to 20% and 2% to 9%, respectively. A significant relationship between fixed N and leaf DM yield was established, reaching 30 kg fixed N t–1 at seed set. Significant variations in leaf atom% 15N excess and %Ndfa quantified decreases in leaf N fixation coupled with N application. Moreover, the petiole content of phenolic compounds markedly increased in the uninoculated control, suggesting deeper investigations on the relationship between bioactive compounds and inoculation treatments. Results highlighted substantial variation in DM, N yields, N-fixation ability, and content of bioactive compounds of sulla leaves caused by inoculation and N fertilization