Repellent activity of essential oils from rutaceae plants against Aedes aegypti (Linn.) and Culex quinquefasciatus (Say)

. Each herbal essential oil in ethyl alcohol was applied at 0.33 µl/cm 2 on the forearms of volunteers. On the protection time (in minutes) and biting rate (%) revealed that essential oil of C. aurantifolia was effective as repellent and feeding against Ae. aegypti (65.0±22.91 minutes protection times and 1.47±0.46% biting rate) and Cx. quinquefasciatus (71.67±5.77 minutes protection times and 1.73±0.23% biting rate). Thus, repellent activity indicated the order of protection time and biting rate against two mosquito species in eight essential oils as C. aurantifolia > C. microcarpa > C. maxima > C. reticulate > C. sinensis > C. hystrix > C. aurantium >C. medica var sarcodaclylis. Unfortunately, the period of protection time against two mosquito species of all herbal essential oil was lower than DEET (155.0±7.07 minutes for Ae. aegypti and 180.0±14.14 minutes for Cx. quinquefasciatus)