ÉTUDE AUTORADIOGRAPHIQUE DE L'ACÉTYLATION DES PROTÉINES NUCLÉAIRES AU COURS DE LA SPERMIOGENÈSE DU CRIQUET

International audienc

ÉVOLUTION DES STRUCTURES NUCLÉAIRES AU COURS DE LA SPERMIOGENÈSE : ORIGINE ET ÉVOLUTION DES CORPS INTRANUCLÉAIRES CHEZ LE CRIQUET LOCUSTA MIGRATORIA

International audienc

3H-actinomycin D binding to ultrathin section of plastic-embedded Locusta migratoria testicular tubules. Improvement of the technique and further characterization of the reaction

Semi-thin and ultrathin sections of locust testes have been incubated in 3H-actinomycin D solution and submitted to radioautography. The improved technical conditions described allow the reproducible obtainment of cell radioautographs with a moderate nuclear labelling and a very low nonspecific background which are usable for semi-quantitative results. Extraction with enzymes (DNase, RNase, pronase) or concentrated salt solution have been carried out before 3H-actinomycin D treatment in order to characterize the reaction. The semi-quantitative results obtained at the light microscope level suggest that, in relation to the structural and chemical changes which occur in chromatin during spermiogenesis, some proteins may be easily hydrolysed in early spermatids. In ultrathin sections of spermatocytes the X chromosome is heavily 'stained' with 3H-actinomycin D, while 3H-uridine is not incorporated into the sex chromatin. These results are discussed in the light of the current ideas on the constitution of active chromatin.SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe

Deoxyribonucleic acid of the crab Cancer pagurus. III. Intracellular localization of poly d(AT) of Cancer pagurus by hybridization in situ

The satellite DNA poly [d(AT) · d(TA)] of the crab Cancer pagurus has been localized in situ by DNA-DNA hybridization in the nuclei of various spermatogenetic, midgut gland, intestinal and tegument cells. The specificity of hybridization was checked by various tests before, during and after hybridization. The nuclear sites revealed by this method were compared with those shown by quinacrine mustard or Giemsa staining. The AT-rich satellite DNA appears to be highly dispersed and does not seem to have any preferential localization inside the crab interphasic nucleus. This situation was compared with that presented by mouse nuclei using similar methods. © 1974.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Autoradiographic demonstration of DNA replication in ultrathin sections of plastic-embedded tissues using an exogeneous DNA polymerase

A DNA-dependent DNA polymerase isolated from regenerating rat liver can mediate the incorporation of tritiated nucleoside triphosphates into acid-insoluble polydeoxyribonucleotides using the DNA contained in ultrathin sections of glycol methacrylate-embedded crab testis as initiator-template. © 1975 Springer-Verlag.SCOPUS: ar.jinfo:eu-repo/semantics/publishe