7 research outputs found
Antibacterial Effects of Carica papaya Fruit on Common Wound Organisms
The purpose of the study was to investigate antibacterial activity of ripe and unripe Carica papaya on selected micro-organisms. Cultures of micro-organisms were routinely maintained in nutrient agar slants at 4掳C. Extracts of immature, mature and ripe Carica papaya fruit were obtained by separately grinding factions of the epicarp, endocarp and seeds and filtering them through gauze. Sensitivity tests were conducted by adding 0.06 ml of extract to agar wells (6mm diameter) prepared from 20 ml agar seeded with 106 cells/ml suspension of one of the eight organisms per plate. The inoculated plates were allowed to equilibrate at 4掳C for 1 hour, incubated at 37掳C for 24 hours, and zones of inhibition measured in millimetres. Anti-bacterial activity was expressed in terms of the radius of zone of inhibition. Seed extracts from the fruit showed inhibition in the following order: B cereus > E coli > S faecalis > S aureus > P vulgaris > S flexneri. No significant difference was found in bacterial sensitivity between immature, mature and ripe fruits. No inhibition zone was produced by epicarp and endocarp extracts. Carica papaya seeds contain anti-bacterial activity that inhibits growth of gram-positive and gram-negative organisms. Observed activity was inde-pendent of stage of fruit maturity. Carica papaya has antibacterial effects that could be useful in treating chronic skin ulcers to promote healing.
Efecto antibacteriano de la fruta Carica papaya en organismos comunes en las heridas
RESUMEN
El prop贸sito del estudio fue investigar la actividad antibacteriana de la Carica papaya, tanto madura como verde, sobre micro-organismos seleccionados. Se mantuvieron cultivos de micro-organismos sistem谩ticamente en slant nutriente de agar a 4掳C. Extractos de Carica papaya inmadura, madura y en saz贸n fueron obtenidos moliendo por separado partes del epicarpio, el endocarpio y las semillas, filtr谩ndolas luego mediante una gasa. Se llevaron a cabo entonces pruebas de sensibilidad a帽adiendo 0.06 ml de extracto a los pozos de agar (6mm de di谩metro) preparados a partir 20 ml de agar sembrados con una suspensi贸n de 106 c茅lulas/ml de uno de los ocho organismos por placa. Las placas inoculadas se dejaron equilibrar a 4掳C por espacio de una hora, fueron incubados a 37掳C durante 24 horas, y las zonas de inhibici贸n medidas en mil铆metros. La actividad antibacteriana fue expresada en t茅rminos del radio de la zona de inhibici贸n. Los extractos de semilla de la fruta mostraron poder de inhibici贸n en el orden siguiente: B cereus > E coli > S faecalis > S aureus > P vulgaris > S flexner. No se encontr贸 diferencia significativa en cuanto a sensitividad bacteriana entre las frutas verdes, maduras y en per铆odo de saz贸n. No se produjo zona de inhibici贸n alguna por parte de los extractos de epicarpio y endocarpio. Las semillas de Carica papaya son portadoras de una actividad bacteriana que inhibe el crecimiento de los organismos gram-positivos y gram-negativos. La actividad observada era independiente de la fase de maduraci贸n de la fruta