Microbial and fermentation profiles, losses and chemical composition of silages of buffel grass harvested at different cutting heights

The present study evaluated the microbial population, fermentation profile, losses and dry matter recovery, and chemical composition of silages of buffel grass at different cutting heights. To evaluate the microbial fermentation dynamics, the treatments resulted from a 4 × 5 factorial combination consisting of 4 cutting heights and 5 fermentation periods, in a completely randomized design with three replications. The fermentation was evaluated at the end of 1, 3, 7, 15 and 30 days. The other characteristics of silages with 30 days were evaluated following a completely randomized design with four treatments, consisting of 4 cutting heights (30, 40, 50 and 60 cm), and five replications. Fermentation period and cutting height effects and interaction between both factors were observed on the populations of lactic acid bacteria (LAB), enterobacteria and molds and yeasts. The peak of development of LAB populations was observed on the seventh day of fermentation for the heights of 40 and 50 cm, with 8.25 and 8.30 log cfu/g, respectively. The pH values of silages ranged with different cutting heights, in which at the height of 50 cm the decrease was most pronounced. However, the pH values were similar between the cutting heights at the end of 30 days of fermentation. Quadratic relationship was observed between lactic acid concentrations and cutting heights. The crude protein content behaved linearly, initially showing 128.5 g/kg DM at 30 cm, decreasing as the cutting heights increased. The neutral detergent fiber and ether extract contents increased linearly with the cutting heights. Based on microbial populations, fermentation, losses and chemical composition, it is recommended to harvest buffel grass for silage from 50 cm on

Desenvolvimento de embriões de camundongas após manutenção em diferentes soluções de manipulação

Avaliou-se a eficácia de duas soluções de manipulação (SM) de embriões de camundongas nos estádios de blastocisto inicial (Bin), mórula compacta grau I (McI) e II (McII), distribuídos aleatoriamente em três tratamentos (T), de acordo com a solução de manutenção. No T1 usou-se PBS modificado (controle); no T2, SME e no T3, SME enriquecida. Os embriões foram mantidos durante quatro horas na solução de manutenção e posteriormente classificados quanto ao estádio de desenvolvimento e à qualidade embrionária. Logo após, foram cultivados em meio TCM 199 e classificados novamente quanto ao estádio de desenvolvimento e à qualidade embrionária. A taxa de desenvolvimento dos embriões após manutenção por quatro horas em solução de manipulação foi menor (P0,05) após o cultivo in vitro. Os embriões McII do T3 tiveram maior desenvolvimento (P<0,05) em relação aos embriões do T1 e T2, indicando o efeito benéfico do enriquecimento da solução SME. Conclui-se que as soluções de manipulação SME e SME enriquecida influenciaram beneficamente o desenvolvimento de embriõe

Erratum: first observation of the rare Bþ → DþKþπ− decay [Phys. Rev. D 93, 051101(R) (2016)]

No abstract available