6 research outputs found
Nano-Immunodetection and Quantification of Mycobacteria in Metalworking Fluids
The accurate detection and enumeration of mycobacteria in metalworking fluids (MWFs) is imperative from an environmental protection and occupational health perspective. We report here on a comparison of the labeling efficiency of nano-immunomagnetic particles (NIMP) and free antibody (FAb) to detect mycobacteria in semisynthetic MWF by using both traditional visualization analysis and cluster analysis aided visualization analysis (CAAVA). The NIMP labeling method involved coating nanometer-scale magnetic particles with Protein A, and oriented conjugation of polyclonal antibodies specific to Mycobacterium spp. The FAb labeling method is modified from the traditional immunofluorescence (IF) method for more efficient detection of mycobacteria in a model MWF. The labeling efficiency of NIMP and FAb were 7.2 ± 4.6 and 16.3 ± 5.5%, and the specificity 85.0 ± 6.1 and 88.1 ± 10.5%, respectively, based on traditional visualization analysis. Based on CAAVA analysis, the labeling efficiency of NIMP and FAb increased to 12.4 ± 1.6 and 20.5 ± 3.9%, and the specificity to 97.8 ± 3.2 and 98.5 ± 2.5%, respectively. A linear relationship of FCM counts and seeded concentrations was observed over four orders of magnitude (R 2 ≤ 0.99) in pure and ternary cultures. The results strongly support the applicability of either FAb or NIMP coupled with CAAVA and flow cytometry for rapid detection and enumeration of mycobacteria in complex matrices.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/63114/1/ees.2007.24.58.pd
Recommended from our members
Enhanced oil recovery by improved waterflooding. Fourth annual report, October 1980-September 1981. [Illinois]
Energy Resourcs Co. Inc., and its subcontractor Elf Aquitaine Oil and Gas Company are conducting a 100-acre pilot polymer flood in the Storms Pool Field near Carmi, in White County, Illinois. The project is a cost-sharing venture with the United States Department of Energy (DOE). Preparation for the polymer flood began in September 1977, and the project is scheduled for completion in December 1983. This report reviews progress during the fourth year of performance (October 1980 through September 1981). The Storms Pool, once highly productive, has yielded over 12 million barrels of oil from the Waltersburg formation since its discovery in 1939. The field has been waterflooded for over 20 years and is now largely in stripper production with high watercuts at most producing wells. Material balance and recent electric logs indicate, however, that there is a substantial volume of movable oil still in place, presumably bypassed by the inefficient waterflood. The polymer flood is intended to improve the sweep efficiency, showing that the engineering, management, and financial resources required for such tertiary techniques can be applied to similar fields that might otherwise be abandoned for lack of investment by parties knowledgeable in enhanced oil technology. Preflush injection and polymer injection were both initiated during this period with total polymer injection now standing at 179,453 barrels (or about 6% pore volume). Laboratory testing has continued throughout the year with the emphasis being on field support (troubleshooting field problems and monitoring the field injection and production systems). No evidence of polymer break-through has been detected at the production wells. Details of the interference testing program and the radiotracer study executed during this period are also presented