SPECTROPHOTOMETRIC DETERMINATION OF BALOFLOXACIN BY ION-PAIR COMPLEXATION REACTION IN THE BULK AND TABLET DOSAGE FORMS

A rapid, sensitive and accurate spectrophotometric method for the determination of balofloxacin, an antibacterial fluoroquinolone has been developed and validated for its analysis in tablet formulation. In this method, balofloxacin is allowed to react with the bromocresol green (BCG) dye in aqueous acidic buffer. The yellow colored complex species was formed that was extracted into organic solvent, dichloromethane. The formed complexes are quantified spectrophotometrically at the absorption maxima at 412 nm. Linearity was observed over the concentration range of 4-30 µg /ml, with the calibration curve having a good coefficient of correlation of 0.9940. The validated method was accurate as found from recovery of 96.65%-101.26% when applied for marketed tablet formulation of balofloxacin, and thus can be used in quality control and its routine analysis