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Compressibility effects in packed and open tubular gas and supercritical fluid chromatography

The influence of the pressure drop on the efficiency and speed of anal. in packed and open tubular supercrit. fluid chromatog. (SFC) is described: methods previously developed to describe the effects of mobile phase compressibility on the performance of open tubular columns in SFC have been extended to packed columns. The Horvath and Lin equation has been used to elucidate the influence of variations in velocity, diffusivity, and capacity factor along the column on the overall efficiency of packed column SFC. In packed columns, in contrast with the situation in open tubular columns, because the diffusion coeffs., the increase in both linear velocity and capacity factor which result from a significant pressure drop cause the plate height to increase along the column. The effect of liq. decompression along the length of the column on the speed of anal. in SFC has been studied and numerical expressions derived which enable calcn. of compressibility correction factors for the plate height. Both the f1 and f2 correction factors remain very close to unity for acceptable pressure drops, which means that the pressure drop has virtually no effect on the no. of plates generated per unit time for an unretained component. For retained species, the decompression of the mobile phase across the column causes the capacity factor to increase and hence leads to increased anal. time