FGB1 and WSC3 are in planta-induced beta-glucan-binding fungal lectins with different functions

In the root endophyte Serendipita indica, several lectin-like members of the expanded multigene family of WSC proteins are transcriptionally induced in planta and are potentially involved in beta-glucan remodeling at the fungal cell wall. Using biochemical and cytological approaches we show that one of these lectins, SiWSC3 with three WSC domains, is an integral fungal cell wall component that binds to long-chain beta 1-3-glucan but has no affinity for shorter beta 1-3- or beta 1-6-linked glucose oligomers. Comparative analysis with the previously identified beta-glucan-binding lectin SiFGB1 demonstrated that whereas SiWSC3 does not require beta 1-6-linked glucose for efficient binding to branched beta 1-3-glucan, SiFGB1 does. In contrast to SiFGB1, the multivalent SiWSC3 lectin can efficiently agglutinate fungal cells and is additionally induced during fungus-fungus confrontation, suggesting different functions for these two beta-glucan-binding lectins. Our results highlight the importance of the beta-glucan cell wall component in plant-fungus interactions and the potential of beta-glucan-binding lectins as specific detection tools for fungi in vivo

The role of small-scale fisheries in Mediterranean coastal communities. An analytical framework for their development.

Small-scale fisheries make an important contribution to nutrition, food security, sustainable livelihoods and poverty alleviation of coastal areas. This paper deals with the integration of small-scale fisheries in coastal communities, their contribution to sustainable development, and some methodological approaches that can be used to carry out this kind of analysis. Concepts as supply chain, blue growth, ecosystem services and multifunctionality are discussed and finally integrated in a framework for the analysis of coastal communities, which are assimilated to a specific pattern of industrial clusters. The approach proposed represents a useful and relatively easy to use instrument supporting the involvement of people, enterprises and institutions for the analysis of the local context and the definition of future strategies, in order to create a favourable environment offering income and employment opportunities to marginalized communities

Residential building and occupant vulnerability to tephra fall

International audiencePlinian and subplinian volcanic eruptions can be accompanied by tephra falls which may last hours or days, posing threats to people, buildings and economic activity. Numerous historical examples exist of tephra damage and tephra casualties. The mechanisms and consequences of roof collapse from static tephra load are an important area of tephra damage requiring more research. This paper contributes to this work by estimating the structural vulnerability of buildings to tephra load based on both analytical studies and observed damage. New studies are presented of roof strengths in the area around Mt. Vesuvius in southern Italy and of field surveys undertaken in other European volcanic locations to assess building vulnerability to tephra fall. The results are a proposed set of new European tephra fall roof vulnerability curves in areas potentially threatened by explosive volcanic eruptions along with comments on the human casualty implications of roof collapse under tephra loading. Some mitigation recommendations are provided

Biodiesel Production From Lignocellulosic Biomass Using Oleaginous Microbes: Prospects for Integrated Biofuel Production

Biodiesel is an eco-friendly, renewable, and potential liquid biofuel mitigating greenhouse gas emissions. Biodiesel has been produced initially from vegetable oils, non-edible oils, and waste oils. However, these feedstocks have several disadvantages such as requirement of land and labor and remain expensive. Similarly, in reference to waste oils, the feedstock content is succinct in supply and unable to meet the demand. Recent studies demonstrated utilization of lignocellulosic substrates for biodiesel production using oleaginous microorganisms. These microbes accumulate higher lipid content under stress conditions, whose lipid composition is similar to vegetable oils. In this paper, feedstocks used for biodiesel production such as vegetable oils, non-edible oils, oleaginous microalgae, fungi, yeast, and bacteria have been illustrated. Thereafter, steps enumerated in biodiesel production from lignocellulosic substrates through pretreatment, saccharification and oleaginous microbe-mediated fermentation, lipid extraction, transesterification, and purification of biodiesel are discussed. Besides, the importance of metabolic engineering in ensuring biofuels and biorefinery and a brief note on integration of liquid biofuels have been included that have significant importance in terms of circular economy aspects.Fil: Chintagunta, Anjani Devi. Vignan’s Foundation for Science, Technology and Research. Department of Biotechnology; IndiaFil: Zuccaro, Gaetano. Institut National de la Recherche Agronomique; Francia. Università degli Studi di Napoli Federico II; ItaliaFil: Kumar, Mahesh. Central Agricultural University; IndiaFil: Kumar, S. P. Jeevan. Indian Institute of Seed Science; India. Directorate of Floricultural Research; IndiaFil: Garlapati, Vijay Kumar. Jaypee University of Information Technology; IndiaFil: Postemsky, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Kumar, N. S. Sampath. Vignan’s Foundation for Science, Technology and Research. Department of Biotechnology; IndiaFil: Chandel, Anuj K.. Universidade de Sao Paulo; BrasilFil: Simal Gandara, Jesus. Universidad de Vigo; Españ

A small organic compound enhances the religation reaction of human topoisomerase I and identifies crucial elements for the religation mechanism

The different steps of the human Top1 (topoisomerase I) catalytic cycle have been analysed in the presence of a pentacyclic-diquinoid synthetic compound. The experiments indicate that it efficiently inhibits the cleavage step of the enzyme reaction, fitting well into the catalytic site. Surprisingly the compound, when incubated with the binary topoisomerase–DNA cleaved complex, helps the enzyme to remove itself from the cleaved DNA and close the DNA gap, increasing the religation rate. The compound also induces the religation of the stalled enzyme–CPT (camptothecin)– DNA ternary complex. Analysis of the molecule docked over the binary complex, together with its chemical properties, suggests that the religation enhancement is due to the presence on the compound of two oxygen atoms that act as hydrogen acceptors. This property facilitates the deprotonation of the 5 DNA end, suggesting that this is the limiting step in the topoisomerase religation mechanism

Emergency planning and mitigation at Vesuvius: A new evidence-based approach

Disasters from explosive volcanic eruptions are infrequent and experience in emergency planning and mitigation for such events remains limited. The need for urgently developing more robust methods for risk assessment and decision making in volcanic crises has become increasingly apparent as world populations continue to expand in areas of active explosive volcanism. Nowhere is this more challenging than at Vesuvius, Italy, with hundreds of thousands of people living on the flanks of one of the most dangerous volcanoes in the world. We describe how a new paradigm, evidence-based volcanology, has been applied in EXPLORIS to contribute to crisis planning and management for when the volcano enters its next state of unrest, as well as in long-term land-use planning. The analytical approach we adopted enumerates and quantifies all the processes and effects of the eruptive hazards of the volcano known to influence risk, a scientific challenge that combines field data on the vulnerability of the built environment and humans in past volcanic disasters with theoretical research on the state of the volcano, and including evidence from the field on previous eruptions as well as numerical simulation modelling of eruptive processes. Formal probabilistic reasoning under uncertainty and a decision analysis approach have provided the basis for the development of an event tree for a future range of eruption types with probability paths and hypothetical casualty outcomes for risk assessment. The most likely future eruption scenarios for emergency planning were derived from the event tree and elaborated upon from the geological and historical record. Modelling the impacts in these scenarios and quantifying the consequences for the circumvesuvian area provide realistic assessments for disaster planning and for showing the potential risk–benefit of mitigation measures, the main one being timely evacuation, but include for consideration protecting buildings against dilute, low dynamic pressure surges, and temporary roof supports in the most vulnerable buildings, as well as hardening infrastructure and lifelines. This innovative work suggests that risk-based methods could have an important role in crisis management at cities on volcanoes and small volcanic islands

The finding of vascular and urinary anomalies in the harvested kidney for transplantation.

INTRODUCTION: In kidney transplantation, anatomical vascular and excretory anomalies may represent causes of failure. Today's surgical techniques have made the most of the organs with anatomic anomalies and iatrogenic injury successfully used for transplantation. MATERIALS AND METHODS: From January 2000 to June 2006, we harvested 230 kidneys, of including 88 kidneys (20%) with vascular, urinary, or vascular-urinary anomalies; 64 kidneys were implanted and 15 were sent to other transplantation centers. Only 9 kidneys were not appropriate for transplantation. RESULTS: All patients who received kidneys with the above-mentioned anomalies were carefully examined after the transplantation and short-term and long-term complications were evaluated with respect to controls without anomalies. DISCUSSION: Renal anatomic anomalies are frequently observed during kidney transplantation and may produce postsurgical complications. However, the presence of these anomalies does not necessarily imply the impossibility of using the kidney for a transplant, especially because of improved surgical techniques. Our experience in transplantation procedures showed that even if kidneys present the above-mentioned anomalies they can still be considered appropriate for transplantation when we perform a correct harvesting/back-table transplant surgery. So vascular and urinary anomalies have to be considered always an incentive to research new surgical solutions and to perform a careful surgical technique

NATURAL AND ANTHROPOGENIC RISKS: PROPOSAL FOR AN INTERDISCIPLINARY GLOSSARY

In the past few years, Italy has been ravaged by catastro- phic events of natural or anthropogenic origin. These events have highlighted, once again, the need to take prevention measures in order to mitigate the damage that similar future events could inflict on the Italian social and economic system. As the costs of these measures might be very high, available resources should be allocated carefully, assigning priority to areas with the highest potential risk. Therefore, estimating the potential damage caused by events expected both in the near future and on a longer timescale in a reliable way might be an important tool for pursuing an effective prevention policy. The growing diversification and extent of urbanised areas and economic activities make the estimation of the above effects increasingly complex. Hence, reliance should be made on a combination of disciplines that may be very different from or that have often developed independently of one another over the years. However, this combination has resulted in discre- pancies in methodological approaches, which have impaired the effectiveness of communication to the authorities in char- ge of prevention policies. This document proposes a common way through which the scientific community may present its arguments and ex- press its opinions about risks at the request of policymakers. In particular, the document proposes the use of a glossary with a view to overcoming “language” barriers among the va- rious disciplines and defining a common lexicon. The construction of a glossary requires the definition of key terms and their mutual relations, which may become a common legacy within a scientific and technical community. Moreover, a glossary may help overcome possible differences in the usage of those terms across the various disciplines, con- sidering both consolidated uses at international level and ope- rational practices at national level. Thus, the proposed glossa- ry is not a mere listing of terms with their definitions. Indeed, the meaning of the definitions depends only on the use of the related terms in the reasoning process and in the opinions that represent the outcome of this process. In this sense, the construction of a glossary requires the adoption of a common approach by the members of the scientific community involved. The document is organised as follows. The second sec- tion provides an overview of the rationale for a common ter- minology and the features that it should have, explaining its logical structure and normative purpose. Section 3 defines the concept of risk analysis in simple terms, highlighting the pos- sible ambiguities associated with this concept that need fur- ther insights. Section 4 proposes a formalisation of the logical process to be followed in carrying out assessments and ex- pressing opinions on various risks. This section also deals, in general terms, with the main items of information considered and their mutual relations. Section 5 presents the glossary, i.e. a list of terms, their definitions, and, where necessary, their application contexts. Finally, a short list of references used in the document is provided

Cord serum cotinine as a biomarker of fetal exposure to cigarette smoke at the end of pregnancy.

This study investigated the association between biomarkers of fetal exposure to cigarette smoke at the end of pregnancy, cotinine in cord serum and in maternal and newborn urine samples, and quantitative measurement of smoking intake and exposure evaluated by maternal self-reported questionnaire. Study subjects were 429 mothers and their newborns from a hospital in Barcelona, Spain. A questionnaire including smoking habits was completed in the third trimester of pregnancy and on the day of delivery. Cotinine concentration in cord serum was associated with daily exposure to nicotine in nonsmokers and with daily nicotine intake in smokers. The geometric mean of cotinine concentration in cord serum statistically discriminated between newborns from nonexposed and exposed nonsmoking mothers, and between these two classes and smokers, and furthermore was able to differentiate levels of exposure to tobacco smoke and levels of intake stratified in tertiles. Urinary cotinine levels in newborns from nonsmoking mothers exposed to more than 4 mg nicotine daily were statistically different from levels in two other categories of exposure. Cotinine concentration in urine from newborns and from mothers did not differentiate between exposure and nonexposure to environmental tobacco smoke (ETS) in nonsmoking mothers. Cord serum cotinine appeared to be the most adequate biomarker of fetal exposure to smoking at the end of pregnancy, distinguishing not only active smoking from passive smoking, but also exposure to ETS from nonexposure