ПРИМЕНЕНИЕ НАСТОЙКИ ПРОПОЛИСА ДЛЯ ПРОФИЛАКТИКИ РЕСПИРАТОРНЫХ ИНФЕКЦИЙ ЦЫПЛЯТ-БРОЙЛЕРОВ

Scientific and industrial experiment was carried out at the poultry farming on Ross308 broilers. The authors explored various schemes of application of propolis tincture for pre venting respiratory infection of poultry of bacterial etiology. The researchers arranged a control group and two experimental groups according to the principle of analogues. The groups were placed in separate isolated poultry houses. Chickens of the control group were fed with antibiotic Tilmipool (0.3 ml/l of water) aged 1-3; 14-16; 25-27 days; their poultry house was sprayed with Ecocide C (0.5%, 1 l/100 m3, exposure 60 min) on 1, 7, 14, 21-22, 28-29, 35-36 days of their life. In the 1st group the antibiotic was applied as it was in the control group; aerosol treatment was conducted with propolis tincture (dilution 1:20, 0.5 l/100 m3, exposure 60 min) during the same age periods. In the 2nd group, propolis tincture (1 ml/l of water) was applied for broilers aged 1-5; 14-18; 25-30 days combined with aerosol treatment of propolis tincture air (dilution 1:20, 0.5 l/ 100 m3, exposure 60 min) for 1, 7, 14, 21-22, 28-29, 35-36 days of their life. The most effective way to prevent respiratory diseases of poultry is seen in application of propolis tincture according to the scheme used in the experiment with the 2nd group. The scheme reduces the number of pathogenic, relatively pathogenic microorganisms in the scrapes from the laryngeal mucous membrane and in the air of the poultry house, activates the immune system and metabolism of poultry, increases livability on 3.0% and live weight on 342.7 g, which eliminates application of antibiotics for preventive measures, providing environmentally safe products of poultry farming.Научно-производственный опыт проводили в условиях птицеводческого хозяйства на цыплятах-бройлерах кросса Росс-308. Изучали различные схемы применения настойки прополиса для профилактики респираторной инфекции птиц бактериальной этиологии. С этой целью из суточных цыплят по принципу аналогов скомплектованы контрольная и две опытные группы, размещенные в отдельных изолированных птичниках. Цыплятам контрольной группы выпаивали антибиотик Тилмипул (0,3 мл/л воды) в возрасте 1-3; 14-16; 25- 27 дней и проводили аэрозольную обработку воздуха птичника Экоцидом С (0,5%, 1 л/100 м3 , экспозиция 60 мин) на 1, 7, 14, 21-22, 28-29, 35-36-й дни жизни. В 1-й группе применяли антибиотик, как в контрольной группе, аэрозольную обработку осуществляли настойкой прополиса (разведение 1:20, 0,5 л/100 м3 , экспозиция 60 мин) в те же возрастные периоды. Во 2-й группе выпаивали настойку прополиса (1мл/л воды) в возрастные периоды 1-5; 14-18; 25-30 дней в сочетании с аэрозольной обработкой воздуха настойкой прополиса (разведение 1:20, 0,5 л/ 100 м3 , экспозиция 60 мин) на 1, 7, 14, 21-22, 28-29, 35- 36-й дни жизни. Наиболее эффективным для профилактики респираторных инфекций птиц является применение настойки прополиса по схеме 2-й группы. Использование данной схемы снижает количество патогенных, условно-патогенных микроорганизмов в соскобах со слизистой оболочки гортани и в воздухе птичника, активизирует иммунную систему и обмен веществ, повышает сохранность на 3,0% и живую массу на 342,7 г, что позволяет исключить применение антибиотиков в профилактических целях, обеспечивая получение экологически безопасной продукции птицеводства

Oxidative stress induced by the Fe2+/ascorbic acid system or model ischemia in vitro: effect of carvedilol and pyridoindole antioxidant SMe1EC2 in young and adult rat brain tissue

New effective strategies and new highly effective neuroprotective agents are being searched for the therapy of human stroke and cerebral ischemia. The compound SMe1EC2 is a new derivative of stobadine, with enhanced antioxidant properties compared to the maternal drug. Carvedilol, a non-selective beta-blocker, possesses besides its cardioprotective and vasculoprotective properties also an antioxidant effect. We compared the effect of carvedilol and SMe1EC2, antioxidants with a similar chemical structure, in two experimental models of oxidative stress in young and adult rat brain tissue. SMe1EC2 was found to improve the resistance of hippocampal neurons to ischemia in vitro in young and even in 18-month-old rats and inhibited formation of protein carbonyl groups induced by the Fe2+/ascorbic acid pro-oxidative system in brain cortex homogenates of young rats. Carvedilol exerted a protective effect only in the hippocampus of 2-month-old rats and that at the concentration 10-times higher than did SMe1EC2. The inhibitory effect of carvedilol on protein carbonyl formation induced by the pro-oxidative system was not proved in the cortex of either young or adult rats. An increased baseline level of the content of protein carbonyl groups in the adult versus young rat brain cortex confirmed age-related changes in neuronal tissue and may be due to increased production of reactive oxygen species and low antioxidant defense mechanisms in the adult rat brain. The results revealed the new pyridoindole SMe1EC2 to be more effective than carvedilol in neuroprotection of rat brain tissue in both experimental models involving oxidative stress

The relationship between the symptoms of female gonococcal infections and serum progesterone level and the genotypes of Neisseria gonorrhoeae multi-antigen sequence type (NG-MAST) in Wuhan, China

The objective of this investigation was to study the relationship between the symptoms of female gonococcal infections and serum progesterone level and the genotypes of Neisseria gonorrhoeae multi-antigen sequence type (NG-MAST) in Wuhan, China. Eighty-one strains of N. gonorrhoeae were harvested from the vaginal discharge of 975 adult females in Wuhan and were genotyped by using NG-MAST. Serum progesterone (P) and estradiol (E2) levels were measured by radio immunoassay (RIA) in 39 gonorrhea-infected patients with slight symptoms (asymptomatic group) and 42 patients with conspicuous symptoms (symptomatic group). The average levels of serum progesterone in the asymptomatic group were significantly higher than in the symptomatic group (p < 0.05), while no significant difference was found in serum estradiol between the two groups. Of 81 wild-type isolates, 50 NG-MAST sequence types were associated with female infections in Wuhan, and N. gonorrhoeae ST2951, ST735, and ST436 were principally found in asymptomatic patients. ST809 and ST369, however, were mainly detected in asymptomatic female subjects. Gonococcal genetic island (GGI)-positive and GGI-negative strains were found in both the asymptomatic group and the symptomatic group. In females with gonococcal infection, high serum progesterone level is associated with the absence of symptoms, but no association was revealed between genotypes and the presence of symptoms. The GGI bears no relation to the absence of symptoms in the patients

The Discovery of LOX-1, its Ligands and Clinical Significance

LOX-1 is an endothelial receptor for oxidized low-density lipoprotein (oxLDL), a key molecule in the pathogenesis of atherosclerosis.The basal expression of LOX-1 is low but highly induced under the influence of proinflammatory and prooxidative stimuli in vascular endothelial cells, smooth muscle cells, macrophages, platelets and cardiomyocytes. Multiple lines of in vitro and in vivo studies have provided compelling evidence that LOX-1 promotes endothelial dysfunction and atherogenesis induced by oxLDL. The roles of LOX-1 in the development of atherosclerosis, however, are not simple as it had been considered. Evidence has been accumulating that LOX-1 recognizes not only oxLDL but other atherogenic lipoproteins, platelets, leukocytes and CRP. As results, LOX-1 not only mediates endothelial dysfunction but contributes to atherosclerotic plaque formation, thrombogenesis, leukocyte infiltration and myocardial infarction, which determine mortality and morbidity from atherosclerosis. Moreover, our recent epidemiological study has highlighted the involvement of LOX-1 in human cardiovascular diseases. Further understandings of LOX-1 and its ligands as well as its versatile functions will direct us to ways to find novel diagnostic and therapeutic approaches to cardiovascular disease

SB 201823-A antagonises calcium currents in central neurons and reduces the effects of focal ischaemia in rats and mice

Original article can be found at: http://stroke.ahajournals.org/ Copyright American Heart Association. [Full text of this article is not available in the UHRA]Peer reviewe