Send Orders of Reprints at [email protected] Peptides As Therapeutics with Enhanced Bioactivity

Abstract: The development of techniques for efficient peptide production renewed interest in peptides as therapeutics. Numerous modifications for improving stability, transport and affinity profiles now exist. Several new adjuvant and carrier systems have also been developed, enhancing the immunogenicity of peptides thus allowing their development as vaccines. This review describes the established and experimental approaches for manufacturing peptide drugs and highlights the techniques currently used for improving their drug like properties

Peptides as therapeutics with enhanced bioactivity

The development of techniques for efficient peptide production renewed interest in peptides as therapeutics. Numerous modifications for improving stability, transport and affinity profiles now exist. Several new adjuvant and carrier systems have also been developed, enhancing the immunogenicity of peptides thus allowing their development as vaccines. This review describes the established and experimental approaches for manufacturing peptide drugs and highlights the techniques currently used for improving their drug like properties

Biophysical characterization of lectin-glycan interactions for therapeutics, vaccines and targeted drug-delivery.

Lectin-glycan interactions play a role in biological processes, host-pathogen interactions and in disease. A more detailed understanding of these interactions is not only useful for the elucidation of their biological function but can also be applied in immunology, drug development and delivery and diagnostics. We review some commonly used biophysical techniques for studying lectin-glycan interactions; namely: frontal affinity chromatography, glycan/lectin microarray, surface plasmon resonance, electrochemical impedance spectroscopy, isothermal titration calorimetry, fluorescent assays, enzyme linked lectin sorbent assay and saturation transfer difference nuclear magnetic resonance spectroscopy. Each method is evaluated on efficiency, cost and throughput. We also consider the advantages and limitations of each technique and provide examples of their application in biology, drug discovery and delivery, immunology, glycoprofiling and biosensing

Electron microscopical investigations of hexagonal phase precipitation in Zn---12 wt% Al---1 wt% Cu and Zn---27 wt% Al---2 wt% Cu alloys

peer reviewedIn Zn---12 wt% Al---1 wt% Cu and Zn---27 wt% Al---2 wt%Cu alloys aged at 100° and 250°C the precipitation of h.c.p. phases was studied by means of transmission electron microscopy. The presence of the metastable ηm and ηx-phases was established using the Moiré pattern method. The ηm → η phase transition was observed. Screw dislocations are visualized in (114) with b logical and {220} in η phase precipitates by means of Moiré fringes.On étudie, par microscopie électronique en transmission, la précipitation de phases H. C dans des alliages Zn-12% en poids Al---1% en poids Cu et Zn---27% en poids Al---2% en poids Cu. La Présence des phases métastables ηm et ηx est établie par la méthode des moirés. On observe la transition de phase ηm → η. Des dislocations vis avec b logical and •220• sont mises en évidence dans le plan (114βbr) des précipités de la phase η par la méthode des franges de moiré.Die Ausscheidung von hexagonalen Phasen in de Legierungen Zn---12 Al---1 Cu und Zn---27 Al---2 Cu (alles in Gew. —%), die bei 100 und 250°C ausgelagert worden waren, wird mittels Durchstrahlungselektronenmikroskopie studiert. Das Vorkommen der metasbilen Phasen ηm und ηx wird über die Auswertung von Moiré-Streifen bestätigt. Die Phasenumwandlung ηm → η wird beobachtet. In den η-Ausscheidungen werden mit Moiré-Streifen Schraubenversetzungen auf (βbr114) mit b mid left angle bracket220right-pointing angle bracket identifiziert

alpha-1,4-Galactosyltransferase-catalyzed glycosylation of sugar and lipid modified Leu-enkephalins

Glycosylation of therapeutic peptides has been reported to improve delivery and targeting of various vaccines and drugs to specific cells/tissues. However, chemical synthesis of complex oligosaccharide derivatives via conventional methods can be challenging due to the need for several orthogonal hydroxyl group protections. Liposaccharyl galactosyltransferase C, a naturally occurring glycosyltransferase enzyme from Neisseria meningitidis, was found to have the ability to transfer a galactosyl moiety to glyco(lipo)peptides. An enzymatic glycosylation of Leu-enkephalin glyco(lipo)peptides was developed and optimized in this study in order to prepare pain regulating peptides with potentially improved central nervous system delivery