13 research outputs found
Annotations to the Devonian Correlation Table, B 124 di 06: Brachiopod biostratigraphy in the Lower Devonian of the Rheinisches Schiefergebirge (Germany) based on the phylogeny ofArduspirifer (Delthyridoidea, Brachiopoda)
Nouvelles informations sur les trilobites de l’Emsien inférieur basal (étage d’Ulmen; Dévonien inférieur) de Reideschbaach (Luxembourg; anticlinorium de Givonne-Éislek; zone rhéno-hercynienne)
CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF A LIPASE FROM A SPECIES OF PSEUDOMONAS
A lipase from a species of Pseudomonas has been cloned and expressed
in E. coli. Variants of this lipase have been generated, using sitespecific
mutagenesis, that have significantly altered k,,,, K, and
substrate specificity. We have undertaken to determine the threedimensional
structure of this enzyme using X-ray crystallography.
Crystals have been obtained and these crystals diffract to 2.5 Angstrom
resolution. We are now in the process of evaluating heavy atom
derivatives to be used to improve the phase information used to calculate
electron density maps
