13 research outputs found

    DEATH AND THE FOOLS

    No full text

    Late complications of intracorneal ring segments: a case report

    No full text

    CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF A LIPASE FROM A SPECIES OF PSEUDOMONAS

    No full text
    A lipase from a species of Pseudomonas has been cloned and expressed in E. coli. Variants of this lipase have been generated, using sitespecific mutagenesis, that have significantly altered k,,,, K, and substrate specificity. We have undertaken to determine the threedimensional structure of this enzyme using X-ray crystallography. Crystals have been obtained and these crystals diffract to 2.5 Angstrom resolution. We are now in the process of evaluating heavy atom derivatives to be used to improve the phase information used to calculate electron density maps
    corecore