Cytogenetic and Array-CGH Characterization of a Complex de novo Rearrangement Involving Duplication and Deletion of 9p and Clinical Findings in a 4-Month-Old Female

Approximately 15 patients with partial trisomy 9p involving de novo duplications have been previously described. Here, we present clinical, cytogenetic, FISH and aCGH findings in a patient with a de novo complex rearrangement in the short arm of chromosome 9 involving an inverted duplication at 9p24→p21.3 and a deletion at 9pter→p24.2. FISH probes generated from BACs selected from the UCSC genome browser were utilized to verify this rearrangement. It is likely that some previously described duplications of 9p may also be products of complex chromosomal aberrations. This report in which FISH and aCGH were used to more comprehensively characterize the genomic rearrangement in a patient with clinical manifestations of 9p duplication syndrome underscores the importance of further characterizing cytogenetically detected rearrangements

Dokumenty standaryzacyjne NATO w zakresie przeciwdziałania minowego oraz ich aktualizacja jako obszar działań naukowych

NATO standardization documents are the basis for action in many areas of NATO military activity and their affiliated organizations. The development of the NATO standardization documentation has been on-going for more than 60 years, and their content provides a reliable basis for joint action in a unified manner, enabling interoperability in many areas of NATO operations. The article is divided into three parts, the first of which deals with the general principles of NATO standardization, the second part describes the standardization documents related to the measurement of the ship’s signatures in aspect of the mine countermeasures and the third describes the participation in research project of NATO — RIMPASSE 2011 Trial. One of the their task was to verify and update NATO standardization documents including AMP-15.Dokumenty standaryzacyjne NATO są podstawą działania w wielu obszarach aktywności wojsk NATO i stowarzyszonych z nimi organizacji. Rozwój dokumentacji standaryzacyjnej NATO trwa od ponad sześćdziesięciu lat i ich zawartość stanowi rzetelną podstawę do wspólnego działania w zunifikowany sposób, umożliwiając interoperacyjność w wielu dziedzinach działań wojsk NATO. Artykuł podzielono na trzy części, z których pierwsza przybliża ogólne zasady normalizacji w NATO, druga zawiera opis dokumentów standaryzacyjnych związanych z pomiarami pól fizycznych okrętu oraz obroną przeciwminową, trzecia zaś opisuje udział w projekcie badawczym NATO — badania RIMPASSE 2011, którego jednym z zadań była weryfikacja i aktualizacja dokumentów standaryzacyjnych NATO, w tym AMP-15

The practical considerations of dose constraints in diagnostic medical departments using ionizing radiation

The term “dose constraint” has been used in radiological protection since 1990. Its practical application has varied from country to country to the present day. The authors of this paper believe that it is an underestimated tool for the purpose of radiological protection. This study aims to estimate local dose constraints for workers in conventional nuclear medicine and radiology departments, on the basis of doses received in the last 3 to 4 years by different groups of workers. The levels of exposure to employees of the nuclear medicine department correspond well to levels in other countries, but for employees belonging to the radiology department, the levels are significantly lower. Unification of the practical application of dose constraints and legislation concerning their specific values would increase the relevance and effectiveness of these operational units. This is a playing field for radiation safety officers for the determination and widespread publication of local dose constraints in their particular medical departments

The influence of carbon dioxide field flooding in mitral valve operations with cardiopulmonary bypass on S100ß level in blood plasma in the aging brain

Mariusz Listewnik,1 Katarzyna Kotfis,2 Paweł Ślozowski,1 Krzysztof Mokrzycki,1 Mirosław Brykczyński1 1Department of Cardiac Surgery, Pomeranian Medical University, Szczecin, Poland; 2Department of Anesthesiology, Intensive Therapy and Acute Intoxications, Pomeranian Medical University, Szczecin, Poland Introduction: The risk of air microembolism during cardiopulmonary bypass (CPB) is high and influences the postoperative outcome, especially in elderly patients. The use of carbon dioxide (CO2) atmosphere during cardiac surgery may reduce the risk of cerebral air microembolism. The aim of our study was to assess the influence of CO2 field flooding on microembolism-induced brain damage assessed by the level of S100ß protein, regarded as a marker of brain damage.Materials and methods: A group of 100 patients undergoing planned mitral valve operation through median sternotomy using standard CPB was recruited for the study. Echocardiography was performed prior to and after the CPB. CO2 insufflation at 6 L/minute was conducted in the study group. Blood samples for S100ß protein analysis were collected after induction of anesthesia, 2 hours after aorta de-clamping, and 24 hours after operation.Results: The S100ß level in blood plasma did not differ significantly between the study and the control group (0.13±0.08 µg/L, 1.12±0.59 µg/L, and 0.26±0.23 µg/L and 0.18±0.19 µg/L, 1.31±0.62 µg/L, and 0.23±0.12 µg/L, P=0.7, 0.14, and 0.78). The mean increase of the S100ß concentration was 13% lower in the group with CO2 protection than in the control group (0.988 µg/L vs 1.125 µg/L), although statistically insignificant. Tricuspid valve annuloplasties (TVAs) had significant impact on the increase in S100ß concentration in the treatment group after 24 hours (TVA [-] 0.21±0.09 vs TVA [+] 0.42±0.42, P=0.05). In patients >60 years, there were significant differences in the S100ß level 2 and 24 hours after the procedure (1.59±0.682 µg/L vs 1.223±0.571 µg/L, P=0.048, and 0.363±0.318 µg/L vs 0.229±0.105 µg/L, P=0.036) as compared with younger patients.Conclusion: The increase in S100ß concentration was lower in the group with CO2 protection than in the control group. Age and an addition of TVA significantly influenced the level of S100ß concentration in the tests performed 2 hours after aortic clamp release. Keywords: mitral valve surgery, S100ß, air microembolism, cardiopulmonary bypass, carbon dioxide insufflatio