Optical control of L-Type Ca2+ channels using a diltiazem photoswitch

L-type Ca2+ channels (LTCCs) play a crucial role in excitation-contraction coupling and release of hormones from secretory cells. They are targets of antihypertensive and antiarrhythmic drugs such as diltiazem. Here, we present a photoswitchable diltiazem, FHU-779, which can be used to reversibly block endogenous LTCCs by light. FHU-779 is as potent as diltiazem and can be used to place pancreatic β-cell function and cardiac activity under optical control

Fluorescence lifetime images and correlation spectra obtained by multidimensional time-correlated single photon counting

Multidimensional time‐correlated single photon counting (TCSPC) is based on the excitation of the sample by a high‐repetition rate laser and the detection of single photons of the fluorescence signal in several detection channels. Each photon is characterized by its arrival time in the laser period, its detection channel number, and several additional variables such as the coordinates of an image area, or the time from the start of the experiment. Combined with a confocal or two‐photon laser scanning microscope and a pulsed laser, multidimensional TCSPC makes a fluorescence lifetime technique with multiwavelength capability, near‐ideal counting efficiency, and the capability to resolve multiexponential decay functions. We show that the same technique and the same hardware can be used for precision fluorescence decay analysis and fluorescence correlation spectroscopy (FCS) in selected spots of a sample

Recording device for natural haptic textures felt with the bare fingertip

The perception of haptic textures depends on the mechanical interaction between a surface and a biological sensor. A texture is apprehended by sliding one’s fingers over the surface of an object. We describe here an apparatus that makes it possible to record the mechanical fluctuations arising from the friction between a human fingertip and easily interchangeable samples. Using this apparatus, human participants tactually scanned material samples. The analysis of the results indicates that the biomechanical characteristics of individual fingertips clearly affected the mechanical fluctuations. Nevertheless, the signals generated for a single material sample under different conditions showed some invariant features. We propose that this apparatus can be a valuable tool for the analysis of natural haptic surfaces