Immunolocalization of zinc transporters and metallothioneins reveals links to microvascular morphology and functions

Published online: 18 July 2022Zinc homeostasis is vital to immune and other organ system functions, yet over a quarter of the world’s population is zinc deficient. Abnormal zinc transport or storage protein expression has been linked to diseases, such as cancer and chronic obstructive pulmonary disorder. Although recent studies indicate a role for zinc regulation in vascular functions and diseases, detailed knowledge of the mechanisms involved remains unknown. This study aimed to assess protein expression and localization of zinc transporters of the SLC39A/ZIP family (ZIPs) and metallothioneins (MTs) in human subcutaneous microvessels and to relate them to morphological features and expression of function-related molecules in the microvasculature. Microvessels in paraffin biopsies of subcutaneous adipose tissues from 14 patients undergoing hernia reconstruction surgery were analysed for 9 ZIPs and 3 MT proteins by MQCM (multifluorescence quantitative confocal microscopy). Zinc regulation proteins detected in human microvasculature included ZIP1, ZIP2, ZIP8, ZIP10, ZIP12, ZIP14 and MT1-3, which showed differential localization among endothelial and smooth muscle cells. ZIP1, ZIP2, ZIP12 and MT3 showed significantly (p < 0.05) increased immunoreactivities, in association with increased microvascular muscularization, and upregulated ET-1, α-SMA and the active form of p38 MAPK (Thr180/Tyr182 phosphorylated, p38 MAPK-P). These findings support roles of the zinc regulation system in microvascular physiology and diseases.Hai B. Tran, Rachel Jakobczak, Adrian Abdo, Patrick Asare, Paul Reynolds, John Beltrame, Sandra Hodge, Peter Zalewsk

Boundary functions on a bounded balanced domain

summary:We solve the following Dirichlet problem on the bounded balanced domain $\Omega$ with some additional properties: For $p>0$ and a positive lower semi-continuous function $u$ on $\partial \Omega$ with $u(z)=u(\lambda z)$ for $|\lambda |=1$, $z\in \partial \Omega$ we construct a holomorphic function $f\in \Bbb O(\Omega )$ such that $u(z)=\int _{\Bbb Dz}|f|^pd \frak L_{\Bbb Dz}^2$ for $z\in \partial \Omega$, where $\Bbb D=\{\lambda \in \Bbb C\:|\lambda |<1\}$