Protective responses of antioxidant enzymes against bisphenol-A induced oxidative stress in Asian Seabass Lates calcarifer

1458-1463Bisphenol A (BPA) is an endocrine disruptor used as plastic monomer and plasticizer widely present in the aquatic environment. In the present study, the effect of BPA on the antioxidant system in Lates calcarifer was documented. Three different concentrations such as 1, 10, 100 µg/l were selected and exposed to fishes for 30 days. After BPA exposure, antioxidant parameters were estimated against control. The levels of marker enzymes were assessed in the gill, muscle, liver and brain tissues. The results showed that the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation (LPx) were increased in treated groups than control. On the other hand, reduced glutathione (GSH) level was decreased in BPA exposed groups compared to control. Based on the results, imbalance in the antioxidant defence system clearly indicated that the BPA toxicity could lead to susceptible oxidative stress in various tissues of the fish L. calcarifer

Screening and partial purification of antifungal metabolite from <i style="mso-bidi-font-style:normal">Streptomyces rochei</i> MSA14: an isolate from marine mining soil of Southwest coast of India.

888-897A total of fourteen actinobacterial strains were isolated from the mining sediment of Manavalakurichi, Southeast coast of India. Primary screening results through agar well diffusion method revealed that 28.57% actinobacterial strains had in vitro antifungal activity. Most potent actinobacterial isolate MSA14 showed strongest inhibitory activity and was identified as Streptomyces rochei through morphological, physiological, biochemical and 16S rRNA gene sequence characteristics. Crude ethyl acetate extract of S. rochei exhibited wide spectrum antifungal activity which was ranged between 12 and 17 mm. Further evaluation of Minimum inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) showed the values ranged from 50 to 200 and 100 to 200 µg/ml, respectively. Partial purification of crude extract through TLC using various gradient solvent system recorded different spots of active principles with the respective Rf values between 0.22 and 0.90. TLC autobiography assay evidenced that, spot with the Rf value of 0.54 had promising antagonistic activity

Investigation on antibacterial, antifungal and cytotoxic properties of chosen mangroves

1769-1777In the present study antibacterial, antifungal and cytotoxic properties of organic solvent extracts of three mangroves viz. Avicennia marina, Acanthus ilicifolius and Excoecaria agallocha were evaluated. Results showed significant variation in bioactivity with respect to mangroves and solvents used for extraction. Antibacterial and antifungal activity of crude extracts revealed that, methanol extracts had wide spectrum antagonistic activity than chloroform and acetone extracts. Antagonistic activity exhibited by methanol extracts were in the following order: E. agallocha > A. marina > A. ilicifolius. Artemia cytotoxicity assay results inferred that, methanol extracts of E. agallocha, A. marina and A. ilicifolius recorded higher toxicity at lower concentration. Phytochemical analysis of crude extracts showed marked variation in its presence. Findings of the present study indicated that, the synergistic effect of active principles within the crude extracts were responsible for promising biological activity. Thus mangroves proved to be a good source of antimicrobial and cytotoxic agent