Factores trombogénicos sistémicos en paciente con síndrome coronario agudo con cinecoronariografía normal y patológica

En la fisiopatología del síndrome coronario agudo (SCA) sin evidencia de lesión ateromatosa oclusiva, podría participar un estado de hipertrombogenicidad sanguínea, generado por factores trombogénicos sistémicos, como los factores de riesgo cardiovascular y los que intervienen en el balance coagulación-anticoagulación, fibrinolisis y formación de fibrina. Objetivo: Estudiar y comparar los factores trombogénicos sistémicos en pacientes con SCA y cinecoronariografía (CCG) normal y patológica.In the pathophysiology of acute coronary syndrome (ACS) without evidence of occlusive atherosclerotic lesion, could participate a state of blood hipertrombogenicidad generated by systemic thrombogenic factors such as cardiovascular risk factors and those involved in the balance coagulation-anticoagulation, fibrinolysis and fibrin formation. Objective: Study and compare systemic thrombogenic factors in patients with ACS and normal and pathological coronary angiography (GCC).Fil: Testasecca, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de InmunologíaFil: Testasecca, A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de InmunologíaFil: Maneschi, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de InmunologíaFil: Fragapane, P.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de InmunologíaFil: Diumenjo, M. S.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Inmunologí

Two different snoRNAs are encoded in introns of amphibian and human L1 ribosomal protein genes.

We previously reported that the third intron of the X.laevis L1 ribosomal protein gene encodes for a snoRNA called U16. Here we show that four different introns of the same gene contain another previously uncharacterized snoRNA (U18) which is associated with fibrillarin in the nucleolus and which originates by processing of the pre-mRNA. The pathway of U18 RNA release from the pre-mRNA is the same as the one described for U16: primary endonucleolytic cleavages upstream and downstream of the U18 coding region produce a pre-U18 RNA which is subsequently trimmed to the mature form. Both the gene organization and processing of U18 are conserved in the corresponding genes of X.tropicalis and H.sapiens. The L1 gene thus has a composite structure, highly conserved in evolution, in which sequences coding for a ribosomal protein are intermingled with sequences coding for two different snoRNAs. The nucleolar localization of these different components suggests some common function on ribosome biosynthesis

A novel small nucleolar RNA (U16) is encoded inside a ribosomal protein intron and originates by processing of the pre-mRNA.

We report that the third intron of the L1 ribosomal protein gene of Xenopus laevis encodes a previously uncharacterized small nucleolar RNA that we called U16. This snRNA is not independently transcribed; instead it originates by processing of the pre-mRNA in which it is contained. Its sequence, localization and biosynthesis are phylogenetically conserved: in the corresponding intron of the human L1 ribosomal protein gene a highly homologous region is found which can be released from the pre-mRNA by a mechanism similar to that described for the amphibian U16 RNA. The presence of a snoRNA inside an intron of the L1 ribosomal protein gene and the phylogenetic conservation of this gene arrangement suggest an important regulatory/functional link between these two components