Bioaugmentation of biofloc system with enzymatic bacterial strains for high health and production performance of Penaeus indicus

Not AvailableThe beneficial effects of two probiotic bacterial strains Marinilactibacillus piezotolerans and Novosphingobium sp. during the culture of Indian white shrimp, Penaeus indicus, under biofloc and clear water system were evaluated. The experimental variation were CW1 (M. piezotolerans in clear water), BFT1 (biofloc + M. piezotolerans), CW2 (Novosphingobium sp. in clear water), BFT2 (biofloc + Novosphingobium sp.) and control (without bacterial strains and biofloc). Growth and survival considerably increased in probiotic bio-augmented treatments. Probiotic incorporation significantly improved water quality, especially ammonia reduction. Microbiota analysis from gut samples taken from different treatments revealed varied microbial population structure among clear water culture, biofloc culture and control. Proteobacteria and Firmicutes were the top phyla observed in the treatments which were significantly higher in bio-augmented systems than the control. Vibrio genera were predominantly observed in control and clear water system compared to that of biofloc systems. Immune genes were significantly altered in response to probiotic gut microbial supplementation than the control. Higher gene expression profile of important immune genes was observed in the biofloc reared shrimps. Expression of digestive enzyme related genes such as trypsin, chymotrypsin, cathepsin L, cathepsin B and alpha amylase were also upregulated significantly in probiotic supplementation especially in the biofloc treatments. Proteomic analysis of hepatopancreas of shrimps from different treatments was carried out by using 2D gel electrophoresis and MALDITOF analysis. The proteins were mostly related to growth and stress tolerance. Eukaryotic initiation factor 4E binding protein was expressed in all the groups and it was high in biofloc treated animals followed by animals treated solely with probiotics compared to those of control groups. The results concludes that biofloc already proved as an effective culture method for healthy shrimp production and supplementation of probiotic bacterial strains registered additional benefit for growth, survival, microbial, immunological status of P, indicus culture.Not Availabl

Enzyme production from food wastes using a biorefinery concept

According to Food and Agricultural Organization (FAO), one-third of food produced globally for human consumption (nearly 1.3 billion tonnes) is lost along the food supply chain. In many countries food waste is currently landfilled or incinerated together with other combustible municipal wastes for possible recovery of energy. However, these two options are facing more and more economic and environmental stresses. Due to its organic- and nutrient-rich nature, theoretically food waste can be converted to valuable products (e.g. bio-products such as methane, hydrogen, ethanol, enzymes, organic acids, chemicals and fuels) through various fermentation processes. Such conversion of food waste is potentially more profitable than its conversion to animal feed or transportation fuel. Food waste valorisation has therefore gained interest, with value added bio-products such as methane, hydrogen, ethanol, enzymes, organic acids, chemicals, and fuels. Therefore, the aim of this review is to provide information on the food waste situation with emphasis on Asia–Pacific countries and the state of the art food waste processing technologies to produce enzymes

Evaluation of Mechanical Behaviour of Multiwalled Nanotubes Reinforcement Particles in Jute-Glass Fibres Hybrid Composites

Fibre-reinforced polymers (FRPs) are composite materials of plastics reinforced with fibres. Cars, sea, aeronautics, and foundation projects progressively utilize fibre-reinforced polymers. This study aims to study the effect of adding multiwalled nanotubes fillers into the hybridized jute-glass FRP composites and their relative properties. This study uses multiwalled nanotubes (MWCNTs), and particles-hybrid jute-glass composites containing jute fibre chopped layer mats, woven glass mats, epoxy resin, and multiwalled nanotubes fillers were created using the hand layup method. After adding multiwalled nanotubes fillers in various weight proportions, the mechanical behaviours of fibre-reinforced polymers were analysed. The mechanical behaviours of laminated composites were tested using the ASTM standard; the following properties are tensile, flexural, and impact strength. The multiwalled nanotubes with 6% wt. attained the maximum mechanical properties compared to the 2 and 4 wt. % of MWCNTs. The E-based specimen contributes the most to the different types of specimens, with a contribution of 24.21% for tensile, 25.03% for flexural, and 24.56% for impact. The microstructures of hybrid composites were studied using a scanning electron microscope

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Not AvailableThe beneficial effects of two probiotic bacterial strains Marinilactibacillus piezotolerans and Novosphingobium sp. during the culture of Indian white shrimp, Penaeus indicus, under biofloc and clear water system were evaluated. The experimental variation were CW1 (M. piezotolerans in clear water), BFT1 (biofloc + M. piezotolerans), CW2 (Novosphingobium sp. in clear water), BFT2 (biofloc + Novosphingobium sp.) and control (without bacterial strains and biofloc). Growth and survival considerably increased in probiotic bio-augmented treatments. Probiotic incorporation significantly improved water quality, especially ammonia reduction. Microbiota analysis from gut samples taken from different treatments revealed varied microbial population structure among clear water culture, biofloc culture and control. Proteobacteria and Firmicutes were the top phyla observed in the treatments which were significantly higher in bio-augmented systems than the control. Vibrio genera were predominantly observed in control and clear water system compared to that of biofloc systems. Immune genes were significantly altered in response to probiotic gut microbial supplementation than the control. Higher gene expression profile of important immune genes was observed in the biofloc reared shrimps. Expression of digestive enzyme related genes such as trypsin, chymotrypsin, cathepsin L, cathepsin B and alpha amylase were also upregulated significantly in probiotic supplementation especially in the biofloc treatments. Proteomic analysis of hepatopancreas of shrimps from different treatments was carried out by using 2D gel electrophoresis and MALDI-TOF analysis. The proteins were mostly related to growth and stress tolerance. Eukaryotic initiation factor 4E binding protein was expressed in all the groups and it was high in biofloc treated animals followed by animals treated solely with probiotics compared to those of control groups. The results concludes that biofloc already proved as an effective culture method for healthy shrimp production and supplementation of probiotic bacterial strains registered additional benefit for growth, survival, microbial, immunological status of P, indicus culture.Not Availabl

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Not AvailableA 60 days nursery-based co-culture of Indian white shrimp, Penaeus indicus along with seaweed, Gracilaria tenuistipitata trial was carried out in Recirculatory Aquaculture System (RAS) of 6000 Liters capacity tank (FRP, reinforced fibre plastics) by using biofloc technology (BFT) to evaluate the growth, survival performance and immune potential. Accordingly a completely randomized experiment was designed with six treatments. These treatments were: T1- Static system (Control), T2 - RAS with BFT, T3 - RAS with Gracilaria, T4 - RAS and BFT customized with bacterial consortium, T5 - RAS with BFT + Gracilaria + bacterial consortium, T6 - Static + Gracilaria + BFT (without consortium and without RAS). Shrimp post larvae (0.08 ± 0.03 g) were stocked at a density of 3000 nos./ton. RAS with BFT along with the seaweed and bacterial consortium showed significantly higher growth and survival compared to control (T1) and treatments without BFT (T3). The control (T1) exhibited significantly lower growth, weight gain, specific growth rate, yield and higher FCR value than that of the RAS + BFT (T2), RAS+ seaweed (T3) and RAS + BFT or their combinations with bacterial consortium (T4 and T5). The treatment having seaweed without BFT (T3) showed higher survival than the static control (T1), but no significant difference in growth. The highest ABW of 4.23 ± 0.39 was obtained in T4 (BFT customized with consortium) followed by T5 (BFT + seaweed + bacterial consortium) and lowest was observed in T1 (2.14 ± 0.4). Similarly, the highest survival (92.33 ± 1.52) and production (10.39 ± 0.24 kg/ton) was observed in T5. Significantly higher NH4-N and NO2-N was accumulated in the control (T1) in comparison with other treatment like T4 and T5. Immune parameters viz., PPO activity, Total Serum Protein and Total Hemocyte Count (THC) showed significantly higher values in all the treatments compared to control. This study establishes the novel concept of incorporation of macroalgae in biofloc based RAS improving the production performance and immune response of shrimp during nursery as well as grow-out phaseNot Availabl

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Experiments were conducted to evaluate the probiotic effect of bio-augmented Bacillus tequilensis AP BFT3 on improving production, immune response, and proteomic changes of Penaeus vannamei reared in a biofloc system. Penaeus vannamei larvae (PL13) were stocked in 100-L tanks at a rate of 100 no per tank to study the effect of B. tequilensis AP BFT3 with and without biofloc (BFT-PRO and PRO). Control tanks devoid of probiotic strain were maintained in a clear water system. The growth and survival considerably increased in probiotic added biofloc reared shrimp than probiotic added clear water reared ones and control. Water quality significantly improved in probiotic added (PRO) and biofloc-probiotics (BFT-PRO) system than control. Microbiological investigations indicate increased heterotrophic bacterial load in BFT-PRO compared to the PRO and control. The quality of the isolated microbes was analyzed in terms of enzyme production, and an abundance of enzyme-producing bacterial population was observed in BFT-PRO shrimp. Immune-related genes were significantly upregulated in BFT-PRO shrimp, followed by the PRO and control. The proteomic data (2D gel electrophoresis and MALDI-TOF) of muscle tissue from the experimental animals identified 11 differentially expressed proteins. The Daxx OS and Lit v 1 tropomyosin was found upregulated in BFT-PRO shrimps. Downregulation of Na+/K+ATPase was observed in biofloc with probiotic-supplied groups. The findings revealed that the BFT system’s efficacy could be improved through the addition of probiotics. The addition of B. tequilensis AP BFT3 as a probiotic in biofloc induced the expression of essential proteins, reducing contracting diseases during culture.Not Availabl