The effects of polycyclic aromatic hydrocarbons on the immune system of fish: A review

International audiencePolycyclic aromatic hydrocarbons are an important class of environmental pollutants that are known to be carcinogenic and immunotoxic. This review summarizes the diverse literature on the effects of these pollutants on innate and acquired immunity in fish and the mechanism of PAH-induced immunotoxicity. Among innate immune parameters, many authors have focused on macrophage activities in fish exposed to polycyclic aromatic hydrocarbons. Macrophage respiratory burst appears especially sensitive to polycyclic aromatic hydrocarbons. Among acquired immune parameters, lymphocyte proliferation appears highly sensitive to polycyclic aromatic hydrocarbon exposure. However, the effects of polycyclic aromatic hydrocarbons on both specific and non-specific immunity are contradictory and depend on the mode of exposure, the dose used or the species studied. In contrast to mammals, fewer studies have been done in fish to determine the mechanism of polycyclic aromatic hydrocarbon-induced toxicity. This phenomenon seems to implicate different intracellular mechanisms such as metabolism by cytochrome P4501 A, binding to the Ah-receptor, or increased intracellular calcium. Advances in basic knowledge of fish immunity should lead to improvements in monitoring fish health and predicting the impact of polycyclic aromatic hydrocarbons on fish populations, which is a fundamental ecotoxicological goal. (c) 2005 Elsevier B.V. All rights reserved

Possible implication of macrophages in the regulation of cytochrome P450 activities in carp (Cyprinus carpio)

International audienceMacrophages play a key role in the regulation of cytochrome P450 activity induced by immunostimulants in mammals. We investigated the effects of immunostimulants (LPS, dextran sulfate and tilorone) on biotransformation and macrophage activities in carp. The major effect of LPS was its capacity to inhibit 3-MC-induced cytochrome P450 activities in the liver and head kidney. Basal phase I activities were reduced by tilorone and dextran sulfate in immune organs. Tilorone and dextran sulfate differently modulated total cytochrome P450 contents and P4501A activities suggesting differential sensitivity for P450 classes. In immune organs, tilorone and dextran sulfate inhibited basal EROD activity. Tilorone inhibited 3-MC-induced EROD activity whereas dextran sulfate enhanced this activity. LPS and dextran sulfate increased ROS production by macrophages and all the immunostimulants induced macrophage activating factor (MAF) production. This study demonstrates for the first time in fish the capacity of CYP-regulated immunostimulants to activate macrophages and provides initial insight into the capacity of macrophages to regulate CYP activity induced by immunostimulants in fish. (c) 2005 Elsevier Ltd. All rights reserved

A microstructural and low-cyle fatigue investigation of weld-repaired heat-resistant cast steels

The multi-pass weld-repair of heat-resistant cast steels is carried out using an automated shielded metal arc welding (SMAW) process, with various filler materials and pre-heating at 400°C. Specimens weld-repaired with a filler material more resistant than the heat-resistant cast steel (over-matching) generally crack within the base metal following the tenth filling pass, whereas specimens buttered with a soft alloy prior to welding remain free of cracks. The high temperature strain-controlled fatigue lifetime of material weld-repaired without buttering is lower than that of bulk initial material. This is due to an increase of the stress amplitude as a result of the so-called over-matching. In the case of material welded following a prior buttering, the fatigue lifetime is reduced because of the stress tri-axiality generated in the thin soft layer which prevents its plastic flow. As a consequence, it is concluded that even though buttering prevents cracking efficiently during welding, it is not acceptable as far as fatigue performance, especially lifetime, is concerned

New investigations on aluminium-steel joining with Nd-YAG laser

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Réalisation d'assemblages acier-aluminium par mouillage réactif induit par laser : approche expérimentale et numérique

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Rupture of GTAW steel-aluminium overlap joint

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