6 research outputs found
Cleaning Oil-Contaminated Vessel By Emulsan Producers (Autochthonous Bacteria)
In a process for cleaning hydrocarbonaceous residues, including
residual petroleum from laboratory made oil-contaminated vessels,
several previously isolated bacteria from Ilam and Paydar oil
reservoirs, were used. The isolated strains were compared with the
standard sample of Acinetobacter calcoaceticus PTCC 1318 from Persian
Type Culture Collection (PTCC). This gram-negative bacterium grows on a
variety of different substrates as sole carbon and energy sources,
including crude oil, soy oil and ethanol. It is oxidase-negative,
non-motile and strictly aerobic. Among the isolated strains, two
autochthonous strains were found to produce an extracellular
emulsifying agent when grown in Mineral Salt Medium containing soy oil,
ethanol or local crude oil. The crude emulsifier of PTCC1318, Paydar-4
and Ilam-1 were concentrated from the cell-free culture fluid by
ammonium sulfate precipitation to yield 1.89 g, 1.78 g and 1.69 g of
bioemulsan, respectively. Although measuring the surface tension (ST)
is not very applicable procedure in case of bioemulsan, but in order to
prove this theory, ST was conducted.Further analysis of purified
emulsion was performed to prove the molecular structure by Carbon13
Nuclear Magnetic Resonance, Proton1Nuclear Magnetic Resonance and
Fourier Transform Infrared Radiation methods. These investigations
showed that the molecular weight of emulsion produced by species
isolated from Ilam and Paydar crude oil reservoirs are comparable with
Acinetobacter calcoaceticus PTCC 1318
Emulsan Analysis Produced by Locally Isolated Bacteria and Acinetobacter Calcoaceticus RAG-1
Growth of previously isolated bacteria from Iranian oil reservoirs on
different carbon and energy sources and under varying conditions have
been used to produce a class of extracellular microbial
protein-associated lipopolysaccharides named emulsan.Several Bacteria
were previously isolated from Iranian oil reservoirs and designated as;
Ilam-1 and Paydar-4. In present study, the isolated strains were
compared with standard sample of Acinetobacter Calcoaceticus RAG-1 from
Persian Type Culture Collection (PTCC 1641), IROST. Among the isolated
strains, two strains were found to produce an extracellular,
emulsifying agent when grown in Mineral Salt Medium containing soya
oil, ethanol or local crude oil. The isolated bacteria were cultured
and further analysed using protein estimation, reducing sugar analysis,
hemolytic activity, surface tension and emulsification activity tests.
The crude emulsifier of RAG-1, PAYDAR-4 and ILAM-1 were concentrated
from the cell-free culture fluid by ammonium sulfate precipitation to
yield 1.89g, 1.78g and 1.69g of bioemulsan respectively. Emulsifying
activity was observed over the entire production process. These
investigations showed that emulsan produced by isolated Iranian crude
oil reservoir were comparable with Acinetobacter Calcoaceticus RAG-1
which is made of carbohydrate backbone as its hydrophilic part
(N-acetyl-D-galactoseamine, N-acetylgalactoseamine uronic acid,
diamino-6-deoxy-D-glucose) and fatty acid chain as its hydrophobic
portion