Antimicrobial Peptides and Surfactant Proteins: Potential New Factors Against Respiratory Tract Infection

Although some vaccines and antibiotics have been very effective in preventing and treating respiratory disease, they have not been fully satisfactory. Recently, components of the innate immune system have been increasingly appreciated for their role in host defense against microbial pathogens. These molecules include lysozyme and lactoferrin, but recent work in cattle, sheep, man and other species have identified new classes of peptides expressed by respiratory epithelial cells that have potent microcidal activity in nanogram quantities. These peptides, termed antimicrobial peptides (AMP), include defensins, cathelicidins and anionic peptides. Some are expressed continuously whereas expression of others is stimulated by infection/inflammation. In calves, we have found that two AMP from the defensin family, tracheal antimicrobial peptide (TAP) and lingual antimicrobial peptide (LAP), are expressed in the newborn and increased in response to Mannheimia (Pasteurella) haemolytica infection. In contrast, sheep beta defensin-1 (SBD-1) is not induced by infection and, in fact, appears decreased during viral infection with paramyxovirus-3 (Pl-3). Decreased SBD-1 by Pl-3 may increase the lung\u27s susceptibility to secondary infections or re-infections. Other innate defense molecules include proteins released with lung surfactant. Surfactant protein A and D (SAD) can opsonize and aggregate respiratory syncytial virus (RSV) and activate alveolar macrophages. Preliminary work suggests that chronic bacterial infections result in reduced SpA and SpD expression and we are currently assessing SAD expression in response to PI-3 and RSV. A long-term goal of our work is to identify ways to up-regulate expression of AMP and/or surfactant proteins in the neonate and at times of stress in older animals in order to reduce microbial colonization. Other investigators are seeking ways to utilize AMP\u27s as a new class of antibiotics

Subclinical microcrania, subclinical macrocrarnia, and fifth-month fetal markers (of growth retardation or edema) in schizophrenia: a co-twin control study of discordant monozygotic twins.

Summary: We tested the hypothesis that gestational injuries in some patients with schozophrenia would leave their mark as a subtle reduction in head circumference (subclinical microcrania). Conclusions: The head circumferences of all subjects were in the normal range. Decreased head circumference in affected MZ co-twins (relative to unaffected MZ co-twin) characteriazes discordant MZ pairs with larger finger-ridge-count differences (i.e., second-trimester fetal-size differences). This study using ideal genetic controls suggests that, while present only in some patients with schizophrenia, the decrease in head circumference is most likely a consequence of in-utero nonshared environmental deleterious events manifesting as groth retardation or as fetal edema and occurring around the fifth prenatal month

The Axially Symmetric Ejecta of Supernova 1987A

Extensive early observations proved that the ejecta of supernova 1987A (SN 1987A) are aspherical. Fifteen years after the supernova explosion, the Hubble Space Telescope has resolved the rapidly expanding ejecta. The late-time images and spectroscopy provide a geometrical picture that is consistent with early observations and suggests a highly structured, axially symmetric geometry. We present here a new synthesis of the old and new data. We show that the Bochum event, presumably a clump of $^{56}$Ni, and the late-time image, the locus of excitation by $^{44}$Ti, are most naturally accounted for by sharing a common position angle of about 14\degree, the same as the mystery spot and early speckle data on the ejecta, and that they are both oriented along the axis of the inner circumstellar ring at 45\degree to the plane of the sky. We also demonstrate that the polarization represents a prolate geometry with the same position angle and axis as the early speckle data and the late-time image and hence that the geometry has been fixed in time and throughout the ejecta. The Bochum event and the Doppler kinematics of the [Ca II]/[O II] emission in spatially resolved HST spectra of the ejecta can be consistently integrated into this geometry. The radioactive clump is deduced to fall approximately along the axis of the inner circumstellar ring and therefore to be redshifted in the North whereas the [Ca II]/[O II] 7300 \AA emission is redshifted in the South. We present a jet-induced model for the explosion and argue that such a model can account for many of the observed asymmetries. In the jet models, the oxygen and calcium are not expected to be distributed along the jet, but primarily in an expanding torus that shares the plane and northern blue shift of the inner circumstellar ring.Comment: To Appear in Ap

Three-dimensional distribution of ejecta in Supernova 1987A at 10 000 days

Due to its proximity, SN 1987A offers a unique opportunity to directly observe the geometry of a stellar explosion as it unfolds. Here we present spectral and imaging observations of SN 1987A obtained ~10,000 days after the explosion with HST/STIS and VLT/SINFONI at optical and near-infrared wavelengths. These observations allow us to produce the most detailed 3D map of H-alpha to date, the first 3D maps for [Ca II] \lambda \lambda 7292, 7324, [O I] \lambda \lambda 6300, 6364 and Mg II \lambda \lambda 9218, 9244, as well as new maps for [Si I]+[Fe II] 1.644 \mu m and He I 2.058 \mu m. A comparison with previous observations shows that the [Si I]+[Fe II] flux and morphology have not changed significantly during the past ten years, providing evidence that it is powered by 44Ti. The time-evolution of H-alpha shows that it is predominantly powered by X-rays from the ring, in agreement with previous findings. All lines that have sufficient signal show a similar large-scale 3D structure, with a north-south asymmetry that resembles a broken dipole. This structure correlates with early observations of asymmetries, showing that there is a global asymmetry that extends from the inner core to the outer envelope. On smaller scales, the two brightest lines, H-alpha and [Si I]+[Fe II] 1.644 \mu m, show substructures at the level of ~ 200 - 1000 km/s and clear differences in their 3D geometries. We discuss these results in the context of explosion models and the properties of dust in the ejecta.Comment: Accepted for publication in Ap

Three-dimensional distribution of ejecta in Supernova 1987A at 10 000 days

Due to its proximity, SN 1987A offers a unique opportunity to directly observe the geometry of a stellar explosion as it unfolds. Here we present spectral and imaging observations of SN 1987A obtained ~10,000 days after the explosion with HST/STIS and VLT/SINFONI at optical and near-infrared wavelengths. These observations allow us to produce the most detailed 3D map of H-alpha to date, the first 3D maps for [Ca II] \lambda \lambda 7292, 7324, [O I] \lambda \lambda 6300, 6364 and Mg II \lambda \lambda 9218, 9244, as well as new maps for [Si I]+[Fe II] 1.644 \mu m and He I 2.058 \mu m. A comparison with previous observations shows that the [Si I]+[Fe II] flux and morphology have not changed significantly during the past ten years, providing evidence that it is powered by 44Ti. The time-evolution of H-alpha shows that it is predominantly powered by X-rays from the ring, in agreement with previous findings. All lines that have sufficient signal show a similar large-scale 3D structure, with a north-south asymmetry that resembles a broken dipole. This structure correlates with early observations of asymmetries, showing that there is a global asymmetry that extends from the inner core to the outer envelope. On smaller scales, the two brightest lines, H-alpha and [Si I]+[Fe II] 1.644 \mu m, show substructures at the level of ~ 200 - 1000 km/s and clear differences in their 3D geometries. We discuss these results in the context of explosion models and the properties of dust in the ejecta.Comment: Accepted for publication in Ap

Photoionized Lines in the X-ray Spectra of SMC X-1

We present a detailed spectral analysis of Chandra/ACIS-S CC mode observations of the massive X-ray binary system SMC X-1. The system was observed during both the high and low X-ray states of the roughly 60-day superorbital period. The continuum spectra during both states are well represented by a power law with photon index $\alpha$=0.9 and a blackbody of kT = 0.15keV. The high state spectra are dominated by the continuum and independent of orbital phase whereas the low state spectra show a strong orbital dependence as well as line emission from O, Ne, Mg, Fe, and Si. This is consistent with the states attributed to disk precession: during the high state X-ray emission is dominated by the compact source which is abrubtly eclipsed and during the low state the compact object is hidden by the disk and a larger, less luminous scattering region is responsible for the X-ray emission. A prominent Ne IX feature places a stringent limit (Log $\xi$ = 2.0-2.5) on the ionization parameter which constrains the wind dynamics of the system. The Fe line fluxes are related linearly to the blackbody fluxes indicating that both originate in the same region or are excited by the same mechanism. There is evidence for structure in the Fe-line that cannot be fully resolved by the current observations. The pulse period measured during our observations, 0.7057147$\pm$0.00000027s shows that the uninterrupted spin-up trend of SMC X-1 continues. We discuss the implications of our results for models of SMC X-1.Comment: 31 pages including 10 figures. Accepted for publication in Ap

Preliminary Spectral Analysis of the Type II Supernova 1999em

We have calculated fast direct spectral model fits to two early-time spectra of the Type-II plateau SN 1999em, using the SYNOW synthetic spectrum code. The first is an extremely early blue optical spectrum and the second a combined HST and optical spectrum obtained one week later. Spectroscopically this supernova appears to be a normal Type II and these fits are in excellent agreement with the observed spectra. Our direct analysis suggests the presence of enhanced nitrogen. We have further studied these spectra with the full NLTE general model atmosphere code PHOENIX. While we do not find confirmation for enhanced nitrogen (nor do we rule it out), we do require enhanced helium. An even more intriguing possible line identification is complicated Balmer and He I lines, which we show falls naturally out of the detailed calculations with a shallow density gradient. We also show that very early spectra such as those presented here combined with sophisticated spectral modeling allows an independent estimate of the total reddening to the supernova, since when the spectrum is very blue, dereddening leads to changes in the blue flux that cannot be reproduced by altering the ``temperature'' of the emitted radiation. These results are extremely encouraging since they imply that detailed modeling of early spectra can shed light on both the abundances and total extinction of SNe II, the latter improving their utility and reliability as distance indicators.Comment: to appear in ApJ, 2000, 54

Dust Production and Particle Acceleration in Supernova 1987A Revealed with ALMA

Supernova (SN) explosions are crucial engines driving the evolution of galaxies by shock heating gas, increasing the metallicity, creating dust, and accelerating energetic particles. In 2012 we used the Atacama Large Millimeter/Submillimeter Array to observe SN 1987A, one of the best-observed supernovae since the invention of the telescope. We present spatially resolved images at 450um, 870um, 1.4mm, and 2.8mm, an important transition wavelength range. Longer wavelength emission is dominated by synchrotron radiation from shock-accelerated particles, shorter wavelengths by emission from the largest mass of dust measured in a supernova remnant (>0.2Msun). For the first time we show unambiguously that this dust has formed in the inner ejecta (the cold remnants of the exploded star's core). The dust emission is concentrated to the center of the remnant, so the dust has not yet been affected by the shocks. If a significant fraction survives, and if SN 1987A is typical, supernovae are important cosmological dust producers.Comment: ApJL accepte

Hubble space telescope observations of high-velocity Lyα and Hα emission from supernova remnant 1987A: The structure and development of the reverse shock

We present two-dimensional line profiles of high-velocity (∼±12,000 km s -1) Lyα and Hα emission from supernova remnant 1987A obtained with the Space Telescope Imaging Spectrograph between 1997 September and 2001 September (days 3869-5327 after the explosion). This emission comes from hydrogen in the debris that is excited and ionized as it passes through the remnant's reverse shock. We use these profiles to measure the geometry and development of the reverse-shock surface. The observed emission is confined within ∼±30° about the remnant's equatorial plane. At the equator, the reverse shock has a radius of ∼75% of the distance to the equatorial ring. We detect marginal differences (6% ± 3%) between the location of the reverse-shock front in the northeast and southwest parts of the remnant. The radius of the reverse shock surface increases for latitudes above the equator, a geometry consistent with a model in which the supernova debris expands into a bipolar nebula. Assuming that the outer supernova debris has a power-law density distribution, we can infer from the reverse-shock emission light curve an expansion rate (in the northeast part of the remnant) of 3700 ± 900 km s -1, consistent with the expansion velocities determined from observations in radio (Manchester et al.) and X-ray (Park et al.; Michael et al.) wavelengths. However, our most recent observation (at day 5327) suggests that the rate of increase of mass flux across the northeast sector of the reverse shock has accelerated, perhaps because of deceleration of the reverse shock caused by the arrival of a reflected shock created when the blast wave struck the inner ring. Resonant scattering within the supernova debris causes Lyα photons created at the reverse shock to be directed preferentially outward, resulting in a factor of ∼5 difference in the observed brightness of the reverse shock in Lyαa between the near and far sides of the remnant. Accounting for this effect, we compare the observed reverse-shock Lyα and Hα fluxes to infer the amount of interstellar extinction by dust as E(B - V) = 0.17 ± 0.01 mag. We also notice extinction by dust in the equatorial ring with E(B - V) ≈ 0.02-0.08 mag, which implies dust-to-gas ratios similar to that of the LMC. Since Hα photons are optically thin to scattering, the observed asymmetry in brightness of Hα from the near and far sides of the remnant represents a real asymmetry in the mass flux through the reverse shock of ∼30%. We discuss future observational strategies that will permit us to further investigate the reverse-shock dynamics and resonant scattering of the Lyα line and to constrain better the extinction by dust within and in front of the remnant.published_or_final_versio

Analysis of the Type IIn Supernova 1998S: Effects of Circumstellar Interaction on Observed Spectra

We present spectral analysis of early observations of the Type IIn supernova 1998S using the general non-local thermodynamic equilibrium atmosphere code \tt PHOENIX}. We model both the underlying supernova spectrum and the overlying circumstellar interaction region and produce spectra in good agreement with observations. The early spectra are well fit by lines produced primarily in the circumstellar region itself, and later spectra are due primarily to the supernova ejecta. Intermediate spectra are affected by both regions. A mass-loss rate of order $\dot M \sim 0.0001-0.001$\msol yr$^{-1}$ is inferred for a wind speed of 100-1000 \kmps. We discuss how future self-consistent models will better clarify the underlying progenitor structure.Comment: to appear in ApJ, 2001, 54