Bio-economic impact of energy and protein level in feed for laying hens raised in organic system

The economic results of organic egg production are largely dependent on the cost of the feed and productive performance. The paper aims to establish the productive performance of laying hens raised under specific conditions specific for the ecological system, and also performing a feeding costs estimation based on mathematical models starting from experimental data required. The hens in the experiment were fed a mixture of concentrated (MC) with 2728 kcal metabolizable energy (ME), 15.85% crude protein (CP), 0.67% lysine, 0.52% methionine + cystine, containing in its structure only fodder types organically certified. Throughout the entire experimental period, chickens have recorded a total MC consumption of 13.240 kg with an average daily consumption between 0.110 and 0.130 kg, when they produced a daily average of 31.55 g mass-egg. The amount of mass-egg produced (y) under this experiment can be predicted based on metabolizable energy intake (x1) and crude protein (x2) using the following mathematical model y=a+b*x1+c*x12+d*x13+e*x14+f*x15+g*x2 (R2=0.99%). Between the values obtained after the experiment and the predicted values obtained using the mathematical relationship, the differences are very small 2.17% at the end of experimental period. The costs incurred by feeding the laying hens (y) organically raised can be predicted using the following mathematical model y=exp(a*x1+b*x2+c*x3+d) (R2=0,96), with a rate of error of less than 2.2%, depending on the amount of mass-egg produced (x1), metabolizable energy intake (x1) and crude protein intake (x2)

Contributions to Knowing the Neural Tube Development in Transylvanian Naked Neck Breed

Abstract. In Romania, the Transylvanian Naked Neck hen breed is considered to be an "endangered" population, reason for which we consider that a special attention should have been given until now. Plymouth Rock breed was imported for the first time to Romania from the Studler Company, France in 1969. This paper is aiming to perform a profound analysis of the development patterns of the neural tube in the two breeds, by measurements carried out at 30, 40, 50, and 60 hours of incubation. Observations show that the closure of the neural canal and its transformation into a tube follows an undulatory pattern, of which positive and negative curls are diametrically opposed in the two breeds, while the development speed during the whole studied period have a relative similar value between the two breeds. We estimate that the two breeds have a good combinative capacity, which recommend the utilization of these genetic materials to obtain hybrids for producing "peasant-type" chicken meat, very well-appreciated by the Europeans between the two World Wars

Antiproliferative Effects of Tetrabuthylammonium Chloride Ionic Liquid on HCT 8 Human Colon Carcinoma Cells

The ionic liquids have attracted a great of attention in the scientific community due to their potential pharmaceutical such as antimicrobial. In this paper, the main objective was the assessment of the cytotoxic effect of tetrabutylammonium chloride against HCT 8 human colon carcinoma cell line. The cells were cultured in 75 cm2 culture flasks  using RPMI medium supplemented with 10% inactivated fetal bovine serum (FBS), penicillin (100 IU/mL) and streptomycin (100 μg/mL) and maintained at 37 °C and 5% CO2. Before achieving viability test, the cells were harvested using trypsin solution (0.25%). Then, the cells were seeded in 24 – well plates at a density of 5 x 105 cells/mL in 100 µL medium/well in order to reach confluence. After 24 h, the medium was replaced with fresh medium containing different concentrations of ionic liquid, respectively, 0.085, 0.17, 0.34, 0.68 and 1.36 mg /mL. Control group contained cells without treatment. Cell proliferation kinetics have been studied at 24 and 48 h after IL treatment, following trypsinization and counting total cells per plate by using a Trypan blue dye and a hemocytometer. Data obtained from the growth kinetics assay shows that the tetrabutylammonium chloride (TBAC) had an inhibitory effect on the growth of cells in a concentration dependent manner. The maximum inhibitory effect on HCT 8 cells it was obtained at 1.36 mg TBAC/mL

Comparative Studies on Slaughter Performances of the European Mouflon (Ovis orientalis musimon Lin.) and the Hybrids Obtained by Crossbreeding between Mouflon and Sheep Breed Tigaie

The aim of this work was to determine slaughter performances and carcass characteristics at European Mouflon (Ovis orientalis musimon Lin.). Also, for studied characteristics were determined the differences between European Mouflon (Ovis orientalis musimon Lin.) and hybrids (F1) obtained by crossbreeding between European Mouflon (Ovis ammon musimon Pal.) and the Romanian sheep breed Tigaie, raised under identical conditions. First generation hybrids (F1) were obtained by crossbreeding between females of sheep breed Tigaie and males of mouflon (FT x MM). The studied characteristics were slaughter yield on hot and chilled carcass, the participation percentage of the cut parts from live weight and from the whole carcass structure. At the age of 90 days 10 lambs were sacrificed (5 mouflons and 5 hybrids FTxMM). For slaughter performances, superior values were obtained from the hybrid lambs. Thus, slaughter yield calculated had higher at FTxMM (P<0.05) as compared with values obtained at mouflon. The participation percentage of the cut parts from the live weight and from the whole carcass structure had the lower values at mouflon lambs compared to FTxMM hybrids. Results from this study showed that hybrid genotype significantly influenced the studied characteristics and have revealed the superiority of the hybrids lambs against mouflon lambs

Corrigendum - Research Regarding the Influence of Sasonality on the Estrus Induction and Synchronization Treatments with PRID, in Dairy Cows

Corrigendum   In the article Research Regarding the Influence of Sasonality on the Estrus Induction and Synchronization Treatments with PRID, in Dairy Cows first published in Scientific Papers: Animal Science and Biotechnologies, Vol 49 (1), by a regrettable error, the hormonal product PRID, produced by CEVA SANTÉ ANIMALE Company, was wrongly assigned to INTERVET Company. We regret the error and assure CEVA SANTÉ ANIMALE Company of our good intentions.  The authors   This article corrects: Research Regarding the Influence of Sasonality on the Estrus Induction and Synchronization Treatments with PRID, in Dairy CowsVol. 49, Issue 1, p. 35-37 Article first published online: 30 May 201

Slaughter Performance and Carcass Characteristics of the Hybrids Obtained by Crossbreeding between European Mouflon (Ovis ammon musimon Pal.) and Sheep Breed Tigaie

The aim of this work was to determine differences between slaughter performances and carcass characteristics of first generation hybrids (F1) obtained by crossbreeding between European Mouflon (Ovis ammon musimon Pal.) and the Romanian sheep breed Tigaie. To assess the yield at slaughter and the participation percentage of the cut parts from the whole carcass structure, crossbreeding were made between females of sheep breed Tigaie and males of mouflon (FT x MM) respectively between mouflon females and males of sheep breed Tigaie (FMxMT). At the age of 60 days 14 lambs were sacrificed (7 hybrids FTxMM and 7 hybrids FMxMT). Slaughter yield calculated on hot and chilled carcasses had higher values at FMxMT (P<0.05). The participation percentage of the cut parts from the whole carcass structure had the highest values at FMxMT hybrids compared to FTxMM hybrids. Results from this study showed that genotype significantly influenced the studied characteristics and have revealed the superiority of FMxMT hybrids as against FTxMM hybrids lambs