Body fat distribution and metabolic consequences — Examination opportunities in dogs

The relationship between metabolic disorders and the distribution of fat in different body regions is not clearly understood in humans. The aim of this study was to develop a suitable method for assessing the regional distribution of fat deposits and their metabolic effects in dogs. Twenty-five dogs were subjected to computed tomographic (CT) imaging and blood sampling in order to characterise their metabolic status. The different fat areas were measured on a cross-sectional scan, and the animals’ metabolic status was evaluated by measuring fasting glucose, insulin and leptin levels. The volume of visceral adipose tissue is the main determinant of leptin levels. The correlation of visceral fat volume and leptin concentration was found to be independent of insulin levels or the degree of insulin resistance. There was a positive correlation between the visceral to subcutaneous fat volume ratio and serum insulin concentration, and a similar trend was observed in the relationship of fat ratio and insulin resistance. The distribution of body fat essentially influences the metabolic parameters in dogs, but the effects of adiposity differ between humans and dogs. The findings can facilitate a possible extrapolation of results from animal studies to humans with regard to the metabolic consequences of different obesity types

Lu-177-labeled zirconia particles for radiation synovectomy

The present article describes the preparation of beta emitter lutetium-177-lebeled zirconia colloid and its preliminary physico-chemical and biological evaluation of suitability for local radionuclide therapy. The new 177Lu-labeled therapeutic radiopharmaceutical candidate was based on the synthesis mode of a previously described zirconia nanoparticle system. Morphology and size of developed radiopharmaceutical compound were evaluated through scanning electron microscope and dynamic light scattering methods. The radiocolloid had a 1.7 micrometer mean diameter and showed suitable radiolabeling and colloid size in vitro stability at RT and during blood sera stability test. After the in vitro characterizations, the product was investigated in the course of the treatment of a spontaneously diseased dog veterinary patient’s hock joint completed with SPECT imaging follow-up measurements and a dual-isotope SPECT imaging tests with conventional 99mTc-MDP bone scintigraphy. In the treated dog any clinical side effects or signs of histopatological alterations of the joints could not be observed during the treatment. SPECT follow-up studies unequivocally showed appropriately high localization of the 177Lu-labeled colloid in hock joint and detectable but negligible radiocolloid leakages in the nearest lymph node, respectively

Pharmacokinetics of selegiline in a rabbit model

Background: Selegiline is one of the most widely used monoamine oxidase B inhibitors for the treatment of Parkinson’s disease in early stage. Objective: Time-dependent distribution of selegiline was monitored in various tissues of rabbits treated with a dose of 30 mg/kg intravenously. Method: Selegiline content was determined by validated HPLC method following 5,15, 30, 60 and 120 minutes of treatment. Results: A time-dependent decline of selegiline tissue levels was observed in the iris, the cornea and the intraocular lens, while the maximum level of selegiline in the serum, in the brain, in the testis and in the retina was found at 15 min following administration

Az ertapenem in vitro hatékonyságának vizsgálata kiterjedt Béta-laktamáz (ESBL) törzsek körében

A kísérletek során arra kerestem a választ, hogy lehet-e az ertapenem az imipinem és meropenem terápiás alternatívája az ESBL termelő baktériumok esetében, továbbá milyen hatékonyságú ez az antibiotikum in vitro vizsgálati környezetben.Mag

Study on medicinal chemistry of k203 in wistar rats and beagle dogs

K203 is an experimental bis-pyridinium mono-aldoxime type cholinesterase reactivator of potential use in organophosphate/ organophosphonate poisoning. Pharmacokinetics of K203 were examined in Wistar rats and beagle dogs using ion-pair HPLC. Serum and cerebrospinal fluid concentrations of K203 were determined using ion-pair reversedphase chromatography on octadecyl silica column. HPLC with ultraviolet detection was used for determination of serum concentration of K203 higher than 0.1 μg/mL while its low concentrations in cerebrospinal fluid required electrochemical detection (0.015 through 4 μg/mL range). In rats the serum levels of K203 followed zero order pharmacokinetics from 15 to 120 minutes post administration. Zero order pharmacokinetics was also observed in beagle dogs after low dose (15 μmol/kg) of K203 administration. High dose administration (250 ?mol/kg) led to subsequent hindered elimination from both cerebrospinal fluid and serum. © 2013 Bentham Science Publishers

The possibility of using mesenchymal stem cells for veterinary research and medicine Part 2 - Own examinations

SUMMARY In the first part of the two-part article series the authors introduced the possibility of the application of mesenchymal stem cell (MSC) in the field of human and principally veterinary medicine. In this second article they present their own results of the in vitro proliferative capacity and osteogenic differentiation potential of adipose tissue MSCs isolated from dogs of different ages. MSC cells were isolated from canine subcutaneous adipose tissue which were proliferated, characterized according to the international standard, differentiated and frozen for following experiments. Their observation revealed that MSCs from old and young donor Beagle dogs proliferated properly in vitro and they were able to show the required fibroblast- like morphology and differentiated into adipo-, chondro- and osteogenic lineages. The osteogenic differentiation of MSCs was followed by the measurement of alkaline phosphatase activity and calcium accumulation. Both of the determined parameters were higher in the case of younger donors compared to the old donor dog