Exotic herbaceous species interact with severe drought to alter soil N cycling in a semi-arid shrubland

Mediterranean-type ecosystems are increasingly threatened by climate change and exotic annual species, jeopardizing the native communities and their global biodiversity. In these systems, soil nitrogen (N) limits net primary production, and its availability can be influenced by both of these stressors. To understand the interactive effects of droughts and exotic herbaceous species on soil N, we monitored the temporal variability of soil inorganic N, net N mineralization, net nitrification, and NO3- leaching under native- and exotic-dominated stands exposed to rainfall manipulation plots in a Mediterranean-type shrub-dominated community. Increasing drought severity resulted in the accumulation of soil NH4+ and NO3-, with a more pronounced increase in exotic-dominated plots. Increased net N mineralization and net nitrification and reduced leaching losses were observed as mechanisms of inorganic N accumulation. In comparison to soils under native plants, soils under exotic plants had enhanced leaching losses upon soil rewetting. We propose that distinct traits of exotic annual herbaceous species associated with higher N inputs, faster turnover, and reduced temporal uptake determine the changes in N cycling in response to droughts. Severe droughts and exotic plants may produce a larger, more vulnerable pool of N that is prone to losses while providing a competitive advantage to promote exotic growth in these N-limited ecosystems

Microbial community dynamics and coexistence in a sulfide-driven phototrophic bloom

© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Bhatnagar, S., Cowley, E. S., Kopf, S. H., Pérez Castro, S., Kearney, S., Dawson, S. C., Hanselmann, K., & Ruff, S. E. Microbial community dynamics and coexistence in a sulfide-driven phototrophic bloom. Environmental Microbiome, 15(1),(2020): 3, doi:10.1186/s40793-019-0348-0.Background: Lagoons are common along coastlines worldwide and are important for biogeochemical element cycling, coastal biodiversity, coastal erosion protection and blue carbon sequestration. These ecosystems are frequently disturbed by weather, tides, and human activities. Here, we investigated a shallow lagoon in New England. The brackish ecosystem releases hydrogen sulfide particularly upon physical disturbance, causing blooms of anoxygenic sulfur-oxidizing phototrophs. To study the habitat, microbial community structure, assembly and function we carried out in situ experiments investigating the bloom dynamics over time. Results: Phototrophic microbial mats and permanently or seasonally stratified water columns commonly contain multiple phototrophic lineages that coexist based on their light, oxygen and nutrient preferences. We describe similar coexistence patterns and ecological niches in estuarine planktonic blooms of phototrophs. The water column showed steep gradients of oxygen, pH, sulfate, sulfide, and salinity. The upper part of the bloom was dominated by aerobic phototrophic Cyanobacteria, the middle and lower parts by anoxygenic purple sulfur bacteria (Chromatiales) and green sulfur bacteria (Chlorobiales), respectively. We show stable coexistence of phototrophic lineages from five bacterial phyla and present metagenome-assembled genomes (MAGs) of two uncultured Chlorobaculum and Prosthecochloris species. In addition to genes involved in sulfur oxidation and photopigment biosynthesis the MAGs contained complete operons encoding for terminal oxidases. The metagenomes also contained numerous contigs affiliating with Microviridae viruses, potentially affecting Chlorobi. Our data suggest a short sulfur cycle within the bloom in which elemental sulfur produced by sulfide-oxidizing phototrophs is most likely reduced back to sulfide by Desulfuromonas sp. Conclusions: The release of sulfide creates a habitat selecting for anoxygenic sulfur-oxidizing phototrophs, which in turn create a niche for sulfur reducers. Strong syntrophism between these guilds apparently drives a short sulfur cycle that may explain the rapid development of the bloom. The fast growth and high biomass yield of Chlorobi-affiliated organisms implies that the studied lineages of green sulfur bacteria can thrive in hypoxic habitats. This oxygen tolerance is corroborated by oxidases found in MAGs of uncultured Chlorobi. The findings improve our understanding of the ecology and ecophysiology of anoxygenic phototrophs and their impact on the coupled biogeochemical cycles of sulfur and carbon.This work was carried out at the Microbial Diversity summer course at the Marine Biological Laboratory in Woods Hole, MA. The course was supported by grants from National Aeronautics and Space Administration, the US Department of Energy, the Simons Foundation, the Beckman Foundation, and the Agouron Institute. Additional funding for SER was provided by the Marine Biological Laboratory

Defining a Benchmark Suite for Evaluating the Import of OWL Lite Ontologies

SemanticWeb tools should be able to correctly interchange ontologies and, therefore, to interoperate. This interchange is not always a straightforward task if tools have different underlying knowledge representation paradigms. This paper describes the process followed to define a benchmark suite for evaluating the OWL import capabilities of ontology development tools in a benchmarking activity in progress in the Knowledge Web European Network of Excellence

Evaluation of CNN architectures for gait recognition based on optical flow maps

This work targets people identification in video based on the way they walk (\ie gait) by using deep learning architectures. We explore the use of convolutional neural networks (CNN) for learning high-level descriptors from low-level motion features (\ie optical flow components). The low number of training samples for each subject and the use of a test set containing subjects different from the training ones makes the search of a good CNN architecture a challenging task.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Universal prototype for adapting from conventional centrifugal fertilizer to variable-rate spreader

Congreso Agroingeniería 2015. Orihuela (Alicante). 1 a 3 de junio de 2015La Agricultura de Precisión constituye un nuevo paradigma en la agricultura de hoy. A objetivos como el aumento de la productividad o de la eficiencia económica, se les unen otros en auge como el respeto medioambiental y un riguroso control en la seguridad alimentaria. En general las técnicas de agricultura de precisión requieren de un mejor conocimiento de las necesidades del cultivo y del suelo, y también del empleo de la maquinaria más adecuada que tenga en cuenta la variabilidad espacial y temporal en las necesidades de los cultivos. En este contexto, este estudio tiene como objetivo principal, el diseño, desarrollo y evaluación de una plataforma universal para controlar una abonadora centrífuga convencional mediante un monitor comercial inicialmente diseñado para el control de barras de pulverización de productos líquidos, lo que permite la adaptación dinámica de la cantidad de fertilizante sólido según las necesidades reales del cultivo. Para el desarrollo del sistema de control fue necesario realizar una caracterización de la abonadora mediante pruebas de calibración del sistema dosificador. También se han desarrollado los distintos módulos de hardware (módulo para el control de los discos, interacción con el sistema de control de líquidos comercial, etc.) necesarios para automatizar las acciones que se realizan durante el funcionamiento de la máquina. Para la gestión del sistema se utiliza la plataforma de hardware libre de bajo coste Arduino que resulta fácilmente adaptable para otras abonadoras, desarrollando un software capaz de gestionar el resto de componentes. Finalmente se monta todo el conjunto a bordo del tractor y se realizan las pruebas de funcionamiento. Se comprobó que el sistema trabaja correctamente, con una respuesta adecuada, consiguiendo automatizar el proceso de variar la dosis durante la marcha de forma eficaz.Como se observa en estos primeros resultados, la utilización de este equipo como elemento importante en una aplicación variable de insumos, puede suponer una reducción económica y del impacto medioambiental de la operación, además de permitir una distribución mas racional del nitrógeno u otros fertilizantes en campo, algo sin duda de especial relevancia en sistemas modernos de producción.Precision Agriculture represents a new paradigm in modern agriculture. To objective such as increasing productivity and economic efficiency, are joined other arising objective as the environmental concern. Precision agriculture techniques require a better understanding of crop and soil needs, and the use of precise machinery that take into account the spatial-temporal variability across fields. In these terms, the goal of this study was the design, development and evaluation of a universal platform to control a conventional disc fertilizer spreader, and thus able to manage the amount of fertilizer on the go. A specific on-field calibration was necessary to determine the spreader settings for a given type of fertilizer. The components of the hardware modules (control system of disks, interaction with sections control system, etc.) capable of automating actions performed in operation of the spreader were developed. The control system was developed based on an open-source and low-cost microcontroller-board (Arduino), which could be adapted easily to other spreader model, and a firmware capable to control the hardware components. Once installed on-board, field tests were provided to investigate the proper functioning and response time (approx. 1 sec) of the control system and achieve automate the process of varying the fertilizer dose on the go. As noted these preliminary results, the utilization of this prototype as an essential part of a variable input application may found an economic reduction in fertilizer operation and environmental impact, and allows the control of nitrogen or other fertilizer in fields, techniques that definitely significant in modern agriculture production systems

Searching for galactic sources in the Swift GRB catalog

Since the early 1990s Gamma Ray Bursts have been accepted to be of extra-galactic origin due to the isotropic distribution observed by BATSE and the redshifts observed via absorption line spectroscopy. Nevertheless, upon further examination at least one case turned out to be of galactic origin. This particular event presented a Fast Rise, Exponential Decay (FRED) structure which leads us to believe that other FRED sources might also be Galactic. This study was set out to estimate the most probable degree of contamination by galactic sources that certain samples of FREDs have. In order to quantify the degree of anisotropy the average dipolar and quadripolar moments of each sample of GRBs with respect to the galactic plane were calculated. This was then compared to the probability distribution of simulated samples comprised of a combination of isotropically generated sources and galactic sources. We observe that the dipolar and quadripolar moments of the selected subsamples of FREDs are found more than two standard deviations outside those of random isotropically generated samples.The most probable degree of contamination by galactic sources for the FRED GRBs of the Swift catalog detected until February 2011 that do not have a known redshift is about 21 out of 77 sources which is roughly equal to 27%. Furthermore we observe, that by removing from this sample those bursts that may have any type of indirect redshift indicator and multiple peaks gives the most probable contamination increases up to 34% (17 out of 49 sources). It is probable that a high degree of contamination by galactic sources occurs among the single peak FREDs observed by Swift.Comment: Published to A&A, 4 pages, 5 figures, this arXiv version includes appended table with all the bursts considered in this stud

Stability of nanoscale secondary phases in an oxide dispersion strengthened Fe-12Cr alloy

Transmission electron microscopy and atom-probe tomography were used to characterize on a near-atomic scale the microstructure and oxide and carbide phases that form during thermo-mechanical treatments of a model oxide dispersion strengthened Fe-12 wt.% Cr-0.4 wt.% Y₂O₃ alloy. It was found that some of the Y-rich nanoparticles retained their initial crystallographic structure but developed a Cr-enriched shell, while others evolved into ternary oxide phases during the initial processing. The Y- and Cr-rich oxide phases formed remained stable after annealing at 1023 K for 96 h. However, the number of Cr-rich carbides appeared to increase, inducing Cr depletion in the matrix.the FP6 Euratom Research and Training Programme on Nuclear Energy (VdC), The Engineering and Physical Sciences Research Council (SLP) and The Royal Society (EAM). The Comunidad de Madrid, through the ESTRUMATCM (MAT-77) programme, and the use of the Chemical Database Service at DaresburyPublicad

Analytical characterization of secondary phases and void distributions in an ultrafine-grained ODS Fe─14Cr model alloy

Proceedings of the: The 14th International Conference on Fusion Reactor Materials (ICFRM-14) was held at the Sapporo Convention Center in Sapporo, Japan from 7 to 12 September 2009.Two model Fe─14Cr alloys, one containing 0.3 wt.% of Y₂O₃ particles, were fabricated by mechanical alloying of Fe and Cr elemental powders under a He atmosphere. They were subsequently consolidated and thermomechanically treated to produce ultra-fine grained materials. Cr-carbides and oxides were found in both alloys. The oxide dispersion-strengthened (ODS) alloy also contained a fine dispersion of nanoparticles, some of them having a Y─O rich core and a Cr-rich shell. Nanometric sized voids were found in both materials, often attached to secondary phases, dislocations and grain boundaries. Their sizes were significantly smaller in the ODS alloy.This research has been supported by the Fusion Energy Materials Science (FEMaS) FP7 coordination action and the IP3 FP6 ESTEEM project under Contract No. 026019. The financial support from European Fusion Development Agreement (Contract No. 09-240), the Ministerio de Innovacion y Ciencia (Project No. ENE 2008-06403-C06-04 and Juan de la Cierva programme) and the Comunidad de Madrid, through the program ESTRUMAT-CM, Grant S-0505/MAT/0077Publicad

Determination of field capacity and yield mapping in olive harvesting using remote data acquisition

Sensors, communication systems and geo-reference units are required to achieve an optimized management of agricultural inputs with respect to the economic and environmental aspects of olive groves. In this study, three commercial olive harvesters were tracked in Spain and Chile using remote and autonomous equipment to determine their time efficiency and field capacity. An experimental methodology for analyzing the data to determine the field capacity and efficiency is proposed, which, along with a conventional methodology, was used to analyze the data to determine field capacity and efficiency. The results of both methodologies are compared to validate the suitability of the experimental methodology. Furthermore, a yield monitor was developed and evaluate using one of the tested olive harvesters. The results show that yield monitoring of olives is possible, but further research is needed to archieve a more reliable methodology