Aegla nebeccana n. sp. (Crustacea, Aeglidae) from Ivaí Basin, Paraná, Brazil

Trombetta, Alana De Souza, Páez, Fernanda Polli, Santos, Sandro, Teixeira, Gustavo Monteiro (2019): Aegla nebeccana n. sp. (Crustacea, Aeglidae) from Ivaí Basin, Paraná, Brazil. Zootaxa 4712 (1): 138-150, DOI: 10.11646/zootaxa.4712.1.1

Aegla buenoi Marcal & Teixeira 2021, n. sp.

Aegla buenoi Marçal & Teixeira n. sp. (Figs 3–5) urn:lsid:zoobank.org:act: F032E974-6EDE-4BA5-8577-17207631A62 Type-material. Holotype: male, Brazil, Paraná, Ibaiti, Paranapanema River basin, Cinzas River sub-basin, Carneiro stream, 23°57′19.71″S, 50°14′16.09″W, altitude 656 m, C.A. da Silva, R.H.C. do Nascimento and R. Rockembacher coll., 17.v.2016 (MZUEL 249). Paratypes: 1 male and 2 females, same data as holotype (MZUEL 245). 3 males and 2 females, Brazil, Paraná, Ibaiti, Paranapanema River basin, Cinzas River sub-basin, tributary of the Carneiro stream, 23°57′27.00″S, 50°14′5.00″W, altitude 620 m, G.M. Teixeira and J.J.S. Rosa coll., 12.vi.2017 (MZUEL 246, genetic vouchers: BOLD access AEGPR016-18, AEGPR017-18, AEGPR018-18, AEGPR019-18, AEGPR020-18). Type-locality. Carneiro stream, city of Ibaiti, Paraná state, Brazil. Geographical distribution. The new species has been collected in two streams within the Cinzas River subbasin, Paranapanema River basin. Both streams are located in the city of Ibaiti, Paraná state, Brazil. Diagnosis. Rostrum triangular, narrow base, reaching distal apex of compound eyes, carinate. Subrostral process well developed, occupying proximal half of subrostral margin, anterior and posterior margins forming obtuse angle (122°). Extra-orbital sinus deep. Anterolateral spines not reaching basal margin of cornea. Epigastric prominences pronounced and oval. Protogastric lobes pronounced. Cervical groove U-shaped. Cardiac area subrectangular. Proximal dorsal margin of dactylus with rudimentary or absent lobe. Palmar crest disciform, outer surface excavated. Subterminal lobe of carpus well defined, pointed. Ventromesial border of cheliped ischium with 4 or 5 tubercles. Anteromesial region of third thoracic sternite tapered. Anterior angle of second abdominal epimeron and ventral angles of third and fourth abdominal epimeron with corneous scale apically. Anterior margin of second abdominal epimeron almost straight. Uropods wide. Description of holotype. Carapace moderately convex, gastric region convex, dorsal surface scabrous, covered with punctations, anterior and posterior branchial areas expanded laterally (CW/CLE = 1.02) (Figs 3A, 4A). Rostrum triangular, narrow base (RBW/LMR = 0.98), reaching distal apex of compound eyes, carinate along entire length, small corneous scales on lateral margins, tip apparently broken; ventral portion of rostrum much higher than dorsal in profile. Rostral carina beginning at level of protogastric lobes, with row of corneous scales extending next to apex. Subrostral process well developed and covered by small setae, occupying proximal half of subrostral margin, tip rounded, anterior and posterior margins forming obtuse angle (122°) (Figs 3B, 4B). Eyestalk and cornea well developed. Orbital and extra-orbital sinuses deep. Orbital sinus U-shaped. Orbital spines well developed, with small terminal corneous scale. Anterolateral spines acuminate apically with small corneous scales terminally, not reaching basal margin of cornea. Epigastric prominences pronounced and oval, with corneous scales. Protogastric lobes pronounced, with corneous scales (Fig. 4B). Gastric area elevated in relation to hepatic lobes and rostrum in lateral view. Demarcation between hepatic lobes well defined. Lateral margins of hepatic lobes with small corneous scales. Cervical groove U-shaped. Transverse dorsal linea sinuous along its extension. Areola rectangular (AH/ [(APM+AAD)/2] = 2.54). Cardiac area subrectangular (TDL/PMC = 1.22). Epibranchial area strongly elongated, anterolateral angle with 2 corneous scales, lateral margin with row of small corneous scales and small setae. Lateral margins of anterior and posterior branchial areas with row of corneous scales and small setae. Chelipeds unequal, left largest. Major cheliped (left). Dactylus (Fig. 4C): dorsal margin and outer surface with small corneous scales, inner surface with setal tufts and scales; dorsal margin without proximal lobe; cutting margin with well-developed lobular basal tooth, followed by row of corneous scales up to distal end; row of small tufts of long setae next to cutting margin; pre-dactylar lobe well developed, rounded, smooth, without corneous scales. Propodus (Fig. 4C): outer surface granular, globose aspect; palmar crest disciform with outer surface excavated, margin poorly serrated, covered by corneous scales; fixed finger cutting margin with well-developed lobular basal tooth, followed by row of corneous scales up to distal end; inner and outer surfaces of fixed finger with rows of long setae tufts next to cutting margin. Carpus (Fig. 4C): dorsal margin with 2 proximal tubercles, 2 median spines, distal tubercle, double-tipped, each tubercle or spine with terminal corneous scale, subterminal lobe well defined, pointed, with small corneous scales, setae apically; inner surface with 2 distinct tubercles with acute corneous scale terminally; outer surface with carpal ridge high, with small corneous scales. Merus (Fig. 4D): dorsolateral edge with distal tubercle, with corneous scale terminally, followed by row of tubercles decreasing in size proximally; ventromesial edge with 2 distal spines, with corneous scale terminally, followed by 3 tubercles of similar size, with corneous scale; ventrolateral border with 2 distal tubercles, with terminal corneous scale, followed by several small tubercles proximally. Ischium (Figs 3C, 4D): dorsolateral edge with spine, with corneous scale terminally; ventromesial border with large proximal tubercle, 3 small median tubercles and large distal tubercle, each with terminal corneous scale; ventrolateral border smooth. Minor cheliped (right) similar to major cheliped except as noted hereafter. Dactylus (Fig. 4E): cutting margin with rudimentary lobular basal tooth. Propodus (Fig. 4E): cutting margin with rudimentary lobular basal tooth. Carpus (Fig. 4E): dorsal margin with 2 proximal tubercles, 2 median spines, distal spine, each with terminal corneous scale; inner surface with 5 tubercles, corneous scale terminally. Merus (Fig. 4F): ventromesial edge with distal spine, followed by 5 tubercles, each with terminal corneous scale. Ischium (Fig. 4F): ventromesial border with proximal tubercle, distal tubercle, each with terminal corneous scale. Second, third, and fourth pereiopods similar. Dactyli, propodi, carpi, meri and ischii with several rows of setal tufts and small scales on surface. Carpi and meri with row of tubercles with terminal corneous scale along dorsal margin. Meri and ischii with long setae concentrated along dorsal margin. Anteromesial region of third thoracic sternite tapered, projecting between coxae of third maxillipeds, with scattered setae. Fourth thoracic sternite with anterolateral angles produced anteriorly, with scattered setae (Fig. 3D). Anterolateral angle of second abdominal epimeron and ventral angles of third, fourth abdominal epimeron well defined, with corneous scale apically. Anterior margin of second abdominal epimeron almost straight (Fig. 3E). Uropods well developed, wide (WU/HWT = 1.04). Pleopods 2–5 absent. Telson divided by longitudinal suture. Anterolateral and posterolateral margins well differentiated. Variations. The rostrum is generally narrow base, but in some paratypes the base is wider than usual (RBW/ LMR = 1.03 ± 0.04; n = 5). In some specimens, the anterolateral spines can reach the basal margin of the cornea. The areola can be subrectangular (AH/[(APM+AAD)/2] = 2.12 ± 0.20; n = 4), trapezoidal (APM/AAD = 1.79 ± 0.10; n = 3), or rectangular (AH/[(APM+AAD)/2] = 2.31; n = 1). The cardiac area varies from subrectangular (TDL/PMC = 1.28 ± 0.06; n = 7) to trapezoidal (TDL/PMC = 1.45; n = 1). The proximal lobe on the dorsal margin of the dactylus of the major and minor chela may be rudimentary (as opposed to absent) (Fig. 5A). The palmar crest is rectangular instead of disciform in small individuals (CLE <12 mm) (Figs 5B–C). The ventromesial border of ischium may present four small tubercles instead of five (Fig. 5D). The anterolateral angle of second abdominal epimeron may be unarmed instead of present a corneous scale as in the holotype. The uropods are narrow (WU/HWT = 0.92 ± 0.03; n = 3) in some specimens. All measurements taken on type-series and morphometric relationships are summarized in Table 1. Biology. Unknown. Etymology. The specific epithet is given in honor of Dr. Sérgio Luiz de Siqueira Bueno, for his outstanding contributions to the taxonomy and knowledge of the biology of aeglids. Molecular data. Sequences of COI generated in this study were deposited in the BOLD database (access numbers AEGPR016-18, AEGPR017-18, AEGPR018-18, AEGPR019-18, AEGPR020-18).Altogether we analyzed 884 base pairs (bp) of COI with no insertions, deletions, or stop-codon. The mean base frequencies were: A = 0.2880, C = 0.1444, G = 0.1537, T = 0.4140. The genetic distance between A. buenoi n. sp. and its congeners ranged from 2.1% to 4.8% (Table 2). Aegla jacutinga showed the lowest divergence relative to A. buenoi n. sp., and the other species. In contrast, A. strinatii was found to be most distantly related to the new species and exhibited higher interspecific variations than the others, with a minimum K2P divergence of 3.8%. Most of the species showed intraspecific distances lower than 0.4%. Aegla castro and A. schmitti, however, presented intraspecific variations higher than 2.6% (Table 2). Regarding species delimitation, the GMYC analysis indicated the presence of 10 independent strains (Fig. 6). Two distinct clusters, with various well-supported subclusters, were obtained. Interestingly, the sequences of A. castro and A. schmitti formed non-monophyletic groups. On the other hand, all sequences of A. buenoi n. sp. were joined in the same cluster, indicating the presence of a single species.Published as part of Marçal, Ingrid Costa, Páez, Fernanda Polli, Souza-Shibatta, Lenice, Sofia, Silvia Helena & Teixeira, Gustavo Monteiro, 2021, Aegla buenoi n. sp. (Decapoda: Anomura): first record of aeglid crab from Cinzas River basin, Brazil, pp. 291-303 in Zootaxa 5005 (3) on pages 294-299, DOI: 10.11646/zootaxa.5005.3.4, http://zenodo.org/record/514177

Aegla abrupta, sp. nov.

Aegla abrupta &lt;i&gt;sp. nov. Mar&ccedil;al and Teixeira&lt;/i&gt; &lt;p&gt; &lt;i&gt;(Figures 7&ndash;10)&lt;/i&gt;&lt;/p&gt; &lt;p&gt; urn:lsid:zoobank.org:act: 8DC448C9-6106-447A-90E0-9B143E120783 &lt;i&gt;Holotype&lt;/i&gt;&lt;/p&gt; &lt;p&gt; Male [CLE 16.2 mm], Brazil, Paran&aacute;, Ortigueira, Paranapanema River basin, Tibagi River sub-basin, Formiga River, 24&deg;11 &lt;i&gt;ʹ&lt;/i&gt; 11.77&rdquo;S, 50&deg;56 &lt;i&gt;ʹ&lt;/i&gt; 28.60&rdquo;W, altitude 725 m, G.M. Teixeira, I.C. Mar&ccedil;al and R.S. Vieira coll., 10 March 2020 (MZUEL 515).&lt;/p&gt; &lt;p&gt; &lt;i&gt;Paratypes&lt;/i&gt;&lt;/p&gt; &lt;p&gt;5 males [CLE 12.8&ndash;24.4 mm] and 8 females [CLE 12.5&ndash;18.2 mm], same data as holotype (MZUEL 499).&lt;/p&gt; &lt;p&gt; &lt;i&gt;Additional material examined&lt;/i&gt;&lt;/p&gt; &lt;p&gt; 4 males [size not recorded] and 6 females [size not recorded], same data as holotype (MZUEL 504, genetic vouchers: Bold Systems access AEGLA 003-21, AEGLA 004-21). 1 male [CLE 16.7 mm], Brazil, Paran&aacute;, Ortigueira, Paranapanema River basin, Tibagi River sub-basin, tributary of the Formiga River, 24&deg;10 &lt;i&gt;ʹ&lt;/i&gt; 56.50&rdquo;S, 50&deg;56 &lt;i&gt;ʹ&lt;/i&gt; 31.50&rdquo;W, altitude 726 m, O.A. Shibatta coll., August 2014 (MZUEL 388). 1 male [CLE 20.1 mm] and 6 females [CLE 13.6&ndash;19.4 mm], Brazil, Paran&aacute;, Ortigueira, Paranapanema River basin, Tibagi River sub-basin, Piquira River, coordinates and coll. unknown, 2 February 2001 (MZUEL 440). 1 male [CLE 20.1 mm] and 1 female [CLE 19.2 mm], same locality, coordinates and coll. unknown, 25 April 2002 (MZUEL 441).&lt;/p&gt; &lt;p&gt; &lt;i&gt;Diagnosis&lt;/i&gt;&lt;/p&gt; &lt;p&gt;Rostrum triangular, narrow base, extending beyond distal apex of compound eyes, carinate along entire length. Subrostral process well developed, occupying proximal half of subrostral margin, anterior and posterior margins forming acute angle. Orbital and extraorbital sinuses deep. Anterolateral spines reaching basal margin of cornea. Epigastric prominences pronounced. Protogastric lobes pronounced. Cervical groove trapezoidal. Areola subrectangular. Epibranchial area shortened. Lobe on proximal dorsal margin of dactylus rudimentary. Carpal ridge high on outer surface of carpus. Ventromesial border of cheliped ischium with 4 or 5 tubercles. Anteromesial region of third thoracic sternite abrupt. Anterior margin of second abdominal epimeron almost straight. Uropods wide.&lt;/p&gt; &lt;p&gt; &lt;i&gt;Description of holotype&lt;/i&gt;&lt;/p&gt; &lt;p&gt;Carapace moderately convex, gastric region convex, dorsal surface scabrous, covered with punctations (Figure 7A). Rostrum triangular, narrow base (RBW/LMR = 0.79), extending beyond distal apex of compound eyes, carinate along entire length, small corneous scales on lateral margins and tip; ventral portion of rostrum much higher than dorsal in profile. Rostral carina beginning at level of protogastric lobes, with 2 rows of corneous scales extending next to apex. Subrostral process well developed, occupying proximal half of subrostral margin, triangular, tip rounded, anterior and posterior margins forming acute angle (88&deg;) (Figure 8A).&lt;/p&gt; &lt;p&gt;Eyestalk and cornea well developed. Orbital and extra-orbital sinuses deep. Orbital sinus U-shaped. Orbital spines well developed, with small terminal corneous scale. Anterolateral spines acuminate apically with small corneous scales terminally, reaching basal margin of cornea.&lt;/p&gt; &lt;p&gt;Epigastric prominences pronounced, with corneous scales. Protogastric lobes pronounced, with corneous scales (Figure 8A). Gastric area elevated in relation to hepatic lobes and rostrum in lateral view. Demarcation between the first and the second hepatic lobes well defined, between the second and the third hepatic lobes weakly delimited. Lateral margins of hepatic lobes with small corneous scales and small setae.&lt;/p&gt; &lt;p&gt;Cervical groove trapezoidal (Figure 8B). Transverse dorsal linea sinuous along its extension. Areola subrectangular (AH/[(APM+AAD)/2] = 2.20). Cardiac area trapezoidal (TDL/PMC = 1.39). Epibranchial area shortened, triangular, anterolateral angle with corneous scale, lateral margin with row of corneous scales and small setae (Figure 8B). Lateral margins of anterior and posterior branchial areas with row of corneous scales and small setae.&lt;/p&gt; &lt;p&gt;Chelipeds unequal. Left cheliped largest (Figure 9 (A,B)). Dactylus: dorsal margin and outer surface with small corneous scales, inner surface with setal tufts and scales. Proximal dorsal margin with rudimentary lobe. Cutting margin with well-developed lobular basal tooth, followed by row of corneous scales to distal end. Row of small tufts of long setae next to cutting margin. Pre-dactylar lobe well developed, rounded, without corneous scales. Propodus: outer surface granular, globose aspect. Palmar crest subdisciform with outer surface excavated, margin poorly serrated, covered by acuminate corneous scales. Cutting margin of fixed finger with well-developed lobular basal tooth, followed by row of corneous scales to distal end. Inner and outer surfaces of fixed finger with rows of long setal tufts next to cutting margin. Carpus: dorsal margin with 2 proximal tubercles, median spine, distal spine longest, each tubercle or spine with small setae and terminal corneous scale, subterminal lobe well defined, pointed, with small corneous scales and setae. Inner surface with large tubercle, long setae next to dorsal margin. Outer surface with carpal ridge high, with small corneous scales. Merus: dorsolateral edge with distal tubercle, with corneous scale terminally, followed by row of tubercles decreasing in size proximally. Ventromesial edge with distal spine, with corneous scale terminally, followed by 4 tubercles of similar size, with corneous scale. Ventrolateral border with distal tubercle, with terminal corneous scale, followed by row of small tubercles. Ischium (Figure 7B): dorsolateral edge with spine, with corneous scale terminally. Ventromesial border with proximal tubercle, 2 median tubercles and large distal tubercle, each with terminal corneous scale. Ventrolateral border smooth.&lt;/p&gt; &lt;p&gt;Minor cheliped (right) similar to major cheliped except as noted hereafter (Figure 9 (C, D)). Dactylus: cutting margin with rudimentary lobular basal tooth. Propodus: cutting margin with rudimentary lobular basal tooth. Ischium: ventromesial border with proximal tubercle, 3 small median tubercles and large distal tubercle, each with terminal corneous scale.&lt;/p&gt; &lt;p&gt;Second, third and fourth pereiopods similar. Dactyli, propodi, carpi, meri and ischii with several rows of setal tufts and small scales on surface. Carpi and meri with row of small tubercles with terminal corneous scale along dorsal margin. Meri with row of small tubercles with terminal corneous scale along ventral margin. Meri and ischii with long setae concentrated along dorsal margin.&lt;/p&gt; &lt;p&gt;Anteromesial region of third thoracic sternite abrupt, projecting between coxae of third maxillipeds, with scattered setae (Figure 7C). Fourth thoracic sternite with anterolateral angles produced anteriorly, with scattered setae (Figure 10A). Pleopods 2&ndash;5 absent. Anterolateral angle of second abdominal epimeron well defined, unarmed (Figure 10B). Ventral angle of third abdominal epimeron well defined with small corneous scales apically. Ventral angle of fourth abdominal epimeron well defined, unarmed. Anterior margin of second abdominal epimeron almost straight (Figures 7D, 10B). Uropods well developed, wide (WU/HWT = 1.04). Telson divided by longitudinal suture. Anterolateral and posterolateral margins well differentiated.&lt;/p&gt; &lt;p&gt; &lt;i&gt;Variations&lt;/i&gt;&lt;/p&gt; &lt;p&gt;The areola can be subrectangular (n = 10), trapezoidal (n = 2) or rectangular (n = 1). The cardiac area varies from subrectangular (n = 9) to trapezoidal (n = 4). The palmar crest may appear rectangular instead of subdisciform. The anteromesial region of third thoracic sternite can be abrupt (n = 8) or tapered (n = 5). The anterolateral angle of second abdominal epimeron may present apical corneous scales instead of being naked as in the holotype. In most specimens, the ventral angle of the third abdominal epimeron is devoid of corneous scales. The uropods are narrow in a few specimens (n = 2). All measurements are summarised in Table 1.&lt;/p&gt; &lt;p&gt; &lt;i&gt;Etymology&lt;/i&gt;&lt;/p&gt; &lt;p&gt; The specific epithet &lsquo;abrupta&rsquo;, from the Latin &lt;i&gt;abruptus&lt;/i&gt;, refers to the abrupt third thoracic sternite of the species.&lt;/p&gt; &lt;p&gt; &lt;i&gt;Distribution&lt;/i&gt;&lt;/p&gt; &lt;p&gt;The new species has been collected in three watercourses within the Tibagi River subbasin, Paranapanema River basin. These watercourses are located in the city of Ortigueira, Paran&aacute; state, Brazil.&lt;/p&gt; &lt;p&gt; &lt;i&gt;Conservation status&lt;/i&gt;&lt;/p&gt; &lt;p&gt; We suggest that &lt;i&gt;Aegla abrupta&lt;/i&gt; sp. nov. be assigned as &lsquo;Vulnerable&rsquo; (VU) under criteria B1, B2ab(iii) as defined by the IUCN (2019). This species has been recorded in three localities (B2a) and the known extent of occurrence is estimated to be less than 500 km 2 (B1). The streams where the specimens of &lt;i&gt;Aegla abrupta&lt;/i&gt; sp. nov. were found are in agricultural and urbanised areas (B2b(iii)).&lt;/p&gt; &lt;p&gt; &lt;i&gt;Remarks&lt;/i&gt;&lt;/p&gt; &lt;p&gt; &lt;i&gt;Aegla abrupta&lt;/i&gt; sp. nov. can be separated from its congeners, mainly by, among other characters, subrostral process well developed (undeveloped in &lt;i&gt;A. strinatii&lt;/i&gt;), occupying proximal half of subrostral margin (on proximal third of subrostral margin in &lt;i&gt;A. lata&lt;/i&gt;, &lt;i&gt;A. jacutinga&lt;/i&gt;, &lt;i&gt;Aegla santosi&lt;/i&gt; sp. nov. and &lt;i&gt;Aegla nanopedis&lt;/i&gt; sp. nov., and occupying median portion of subrostral margin in &lt;i&gt;A. jaragua&lt;/i&gt;); anterior and posterior margins of subrostral process forming acute angle (right angle in &lt;i&gt;A. lata&lt;/i&gt; and &lt;i&gt;A. jacutinga&lt;/i&gt;, and obtuse angle in &lt;i&gt;A. jaragua&lt;/i&gt;, &lt;i&gt;Aegla santosi&lt;/i&gt; sp. nov. and &lt;i&gt;Aegla nanopedis&lt;/i&gt; sp. nov.); orbital and extra-orbital sinuses deep (shallow in &lt;i&gt;A. odebrechtii&lt;/i&gt;); epigastric prominences pronounced (poorly pronounced in &lt;i&gt;A. lata&lt;/i&gt; and &lt;i&gt;A. jacutinga&lt;/i&gt;); protogastric lobes pronounced (poorly pronounced in &lt;i&gt;A. odebrechtii&lt;/i&gt;, &lt;i&gt;A. lata&lt;/i&gt;, &lt;i&gt;A. jarai&lt;/i&gt; and &lt;i&gt;A. jaragua&lt;/i&gt;); cervical groove trapezoidal (U-shaped in &lt;i&gt;A. lata&lt;/i&gt; and &lt;i&gt;Aegla nanopedis&lt;/i&gt; sp. nov.); subterminal lobe of carpus well defined, pointed (poorly defined in &lt;i&gt;A. strinatii&lt;/i&gt;); outer surface of carpus with carpal ridge high (low in &lt;i&gt;A. jacutinga&lt;/i&gt;); dorsal margin of carpus and merus of second pereiopods with small tubercles with terminal corneous scale (with distal spine followed by tubercles decreasing in size proximally in &lt;i&gt;A. jarai&lt;/i&gt;); ventral margin of merus of second pereiopods with small tubercles with terminal corneous scale (one or two small spines in &lt;i&gt;A. castro&lt;/i&gt; and &lt;i&gt;A. schmitti&lt;/i&gt;); anteromesial region of third thoracic sternite abrupt (tapered or truncate in &lt;i&gt;A. lata&lt;/i&gt;, truncate in &lt;i&gt;A. jaragua&lt;/i&gt;, and tapered in &lt;i&gt;A. jacutinga&lt;/i&gt;, &lt;i&gt;Aegla santosi&lt;/i&gt; sp. nov. and &lt;i&gt;Aegla nanopedis&lt;/i&gt; sp. nov.); anterior margin of second abdominal epimeron almost straight (concave in &lt;i&gt;A. castro&lt;/i&gt; and &lt;i&gt;A. schmitti&lt;/i&gt; and slightly concave in &lt;i&gt;A. jacutinga&lt;/i&gt;); uropods wide (narrow in &lt;i&gt;A. lata&lt;/i&gt; and &lt;i&gt;A. jaragua&lt;/i&gt;).&lt;/p&gt; &lt;p&gt; Our Bayesian tree based on the COI mitochondrial gene (Figure 6) recovered &lt;i&gt;Aegla abrupta&lt;/i&gt; sp. nov. as the sister species to &lt;i&gt;Aegla nanopedis&lt;/i&gt; sp. nov., and both clustered in the same subclade as &lt;i&gt;A. jacutinga&lt;/i&gt;. The interspecific genetic distances (Table 2) reinforce the relationships evidenced in the Bayesian analysis.&lt;/p&gt;Published as part of &lt;i&gt;Marçal, Ingrid Costa, Páez, Fernanda Polli, Silva, Priscila Frazato Da, Souza-Shibatta, Lenice &amp; Teixeira, Gustavo Monteiro, 2021, Cryptic diversity among populations of Aegla Leach, 1820 (Decapoda: Anomura: Aeglidae) from Tibagi River basin, Paraná state, Brazil, with descriptions of three new species, pp. 2145-2171 in Journal of Natural History 55 (33 - 34)&lt;/i&gt; on pages 2155-2161, DOI: 10.1080/00222933.2021.1984599, &lt;a href="http://zenodo.org/record/5727887"&gt;http://zenodo.org/record/5727887&lt;/a&gt

Aegla okora Páez & Marçal & Souza-Shibatta & Gregati & Sofia & Teixeira 2018, n. sp.

&lt;i&gt;Aegla okora&lt;/i&gt; P&aacute;ez &amp; Teixeira n. sp. &lt;p&gt;(Figs 2, 3, 4, 5, 6,)&lt;/p&gt; &lt;p&gt; &lt;b&gt;Type-material&lt;/b&gt;. &lt;b&gt;Holotype:&lt;/b&gt; Male (CLE 21.3 mm), Brazil, Paran&aacute;, Pinh&atilde;o, Igua&ccedil;u River basin, Tapera River, 25&deg;41&rsquo;39.51&rdquo;S, 51&deg;40&rsquo;13.23&rdquo;W, G.M. Teixeira, F.P. Paez and R.A. Gregati coll., February 2018 (MZUEL 250). &lt;b&gt;Paratypes:&lt;/b&gt; 10 males (CLE 10.4 &macr;23.0 mm) and 10 females (CLE 13.0&macr;18.0 mm), same data as holotype (MZUEL 251, genetic voucher: Bold Systems access AEGBR001-18, AEGBR002-18, AEGBR003-18, AEGBR004-18).&lt;/p&gt; &lt;p&gt; &lt;b&gt;Type-locality.&lt;/b&gt; Tapera River, city of Pinh&atilde;o, Paran&aacute;, Brazil.&lt;/p&gt; &lt;p&gt; &lt;b&gt;Geographical distribution.&lt;/b&gt; Known only from the type-locality, despite searching in five nearby streams within a radius of the 32 km.&lt;/p&gt; &lt;p&gt; &lt;b&gt;Diagnosis.&lt;/b&gt; Triangular rostrum with narrow base. Subrostral process developed, anteriorly oriented at a 45&deg; with the rostrum. Epigastric prominences and protogastric lobes pronounced, with scales and small setae. Anterolateral spine reaching basal margin of cornea. Branchial region swollen. Areola trapezoidal. Cardiac area trapezoidal. Proximal dorsal margin of movable finger of cheliped without lobe. Palmar crest of major cheliped rectangular. Anterolateral angle of second abdominal epimeron unarmed, with setae. Ventromesial border of ischium of the cheliped ornate with three tubercles, one proximal, one median and one distal. Uropodal (endopods) wide.&lt;/p&gt; &lt;p&gt; &lt;b&gt;Description of male holotype&lt;/b&gt;. Carapace convex, branchial region swollen. Rostrum triangular, base narrow (RBW/LMR = 0.87), extending beyond distal apex of compound eyes, with scales on lateral margins. Rostral carina beginning at level of protogastric lobes, with two parallel rows of scales becoming one row on distal third near apex (Fig. 2A, 3, 4A). Subrostral process developed forming angle of 45&deg; (Fig. 4B).&lt;/p&gt; &lt;p&gt;Eyestalk and cornea well developed. Orbital and extra-orbital sinuses deep. Orbital sinus with scales. Orbital spines developed, rounded. Anterolateral spines with corneous scales on lateral margin of carapace, reaching basal margin of cornea (Fig. 4A).&lt;/p&gt; &lt;p&gt;Epigastric prominences pronounced, with scales and short setae. Protogastric lobes pronounced, with scales. Gastric area prominently inflated in relation to hepatic lobe and rostrum in lateral view. Demarcation between hepatic lobes well defined. Lateral margins of first hepatic lobe with corneous scale, second and third hepatic lobes with sparse scales.&lt;/p&gt; &lt;p&gt;Cervical groove U-shaped (Fig. 4C). Transverse dorsal linea slightly sinuous throughout its extension, sinuosity more pronounced in mesial section. Areola trapezoidal (APM/AAD = 2.5). Cardiac area trapezoidal (TDL/PMC = 1.53) (Fig. 4C).&lt;/p&gt; &lt;p&gt;Epibranchial area with spine or well developed scales on apex. Lateral margin of anterior branchial area with distal spine, setae and scales, posterior area with setae and scales.&lt;/p&gt; &lt;p&gt;Anteromesial region of third thoracic sternite abrupt, with scattered setae on surface. Fourth thoracic sternite elevated in median region with setae, anterolateral angles well developed with spines (Fig. 2B, 4D).&lt;/p&gt; &lt;p&gt; Chelipeds unequal in size (Figs 2A, 3). Major cheliped (left) (Fig. 5A). Dactylus: dorsal margin and outer surface granulate and ornamented with short scales. Pre-dactylar lobe absent. Proximal lobe on dorsal margin absent. Cutting margin with lobular basal tooth well developed proximally, with flattened corneous scales, followed by row of wide corneous scales up to distal end. Propodus: outer surface granulate. Palmar crest rectangular with outer surface excavated, margin with scales. Cutting margin of fixed finger with flattened corneous scales over its entire surface, with lobular basal tooth well developed proximally and acuminate corneous scale on distal end. Scattered tufts of long setae over inner surface, and alongside inner and outer surfaces next to cutting margin. Scattered scales and scales clustered into groups of 2 or 3 on inner surface. Carpus: dorsal margin with two tubercles proximally, two median spines with terminal corneous scale, one tubercle, internally displaced from the margin, distally, with terminal corneous scale, and sub-terminal lobe well defined, pointed, with corneous scales and setae apically. Inner surface with three tubercles with terminal corneous scale and setae. Outer surface with carpal ridge elevated along entire length, with scales clustered into groups of 3 &lt;b&gt;&ndash;&lt;/b&gt; 5. Merus: dorsal margin with one tubercle. Dorsolateral edge with row of corneous scales and tubercles with corneous scales on distal third. Ventromesial edge with five tubercles decreasing in size proximally. Ventrolateral border with two tubercles distally, followed by row of scales clustered into groups of 2 &lt;b&gt;&ndash;&lt;/b&gt; 3. Ischium: dorsolateral edge with distal spine with terminal corneous scale. Ventromesial border ornamented with one proximal tubercle, one median tubercle and one distal tubercle with one terminal corneous scale each (Fig. 2C, 6A).&lt;/p&gt; &lt;p&gt;Minor chelipeds (right) similar to major chelipeds except as noted hereafter (Fig. 2D, 5B, 6B): Propodus: palmar crest rectangular to subdisciform. Merus: ventromesial edge with four tubercles decreasing in size proximally.&lt;/p&gt; &lt;p&gt;Second, third and fourth pereiopods morphologically similar; general surface of dactylus, propodus and carpus with longitudinal lines of short setae and scales; dorsal margin of merus and ischium with long tufts setae; ventral margin of ischium with tufts of setae.&lt;/p&gt; &lt;p&gt;Pleopods 2&ndash;5 absent.&lt;/p&gt; &lt;p&gt;Anterolateral angle of second abdominal epimeron unarmed, with small setae (Fig. 2E, 6C). Anterior margin of second abdominal epimeron slightly concave.&lt;/p&gt; &lt;p&gt;Uropods wide (WU/HWT = 1.18). Telson divided by longitudinal suture (Fig. 6D).&lt;/p&gt; &lt;p&gt; &lt;b&gt;Variations.&lt;/b&gt; Anterolateral angle of the carapace projected with a conical spine, protruding anteriorly, may just reach basal margin of the cornea. Of the 20 paratypes analyzed, the spine in 13 individuals is longer, extending beyond the basal margin of the cornea.&lt;/p&gt; &lt;p&gt;The shape of the cardiac area may vary in some specimens, being trapezoidal (n = 15) or subrectangular (n = 5).&lt;/p&gt; &lt;p&gt;The third thoracic sternite may vary from abrupt (n = 13) to tapered (n = 7).&lt;/p&gt; &lt;p&gt;Uropods may vary between narrow (n = 9) and wide (n = 11).&lt;/p&gt; &lt;p&gt; &lt;b&gt;Biology.&lt;/b&gt; Unknown.&lt;/p&gt; &lt;p&gt; &lt;b&gt;Etymology.&lt;/b&gt; The specific epithet &ldquo;okora&rdquo;, from the indigenous Kaingang language &ldquo;ȍkor&rdquo; means &ldquo;pine cone seed in the water&rdquo;, refers Pinh&atilde;o City where the type-locality is located. It is a noun in apposition.&lt;/p&gt; &lt;p&gt; &lt;b&gt;Molecular data.&lt;/b&gt; A total of 511 bp of COI were analyzed. No insertions, deletions or stop-codons were detected, indicating that all amplified regions correspond to a functional portion of the COI gene.&lt;/p&gt; &lt;p&gt; The genetic distance between &lt;i&gt;Aegla okora&lt;/i&gt; &lt;b&gt;n. sp.&lt;/b&gt; and the other species included in the analysis ranged from 0.017 to 0.041 (Tab. 2). &lt;i&gt;Aegla parana&lt;/i&gt; e &lt;i&gt;A. schmitti&lt;/i&gt; presented the smallest genetic distance from A. okora &lt;b&gt;n. sp.&lt;/b&gt;, with a value of 0.017 for both. On the other hand, &lt;i&gt;A. meloi&lt;/i&gt; was most divergent from &lt;i&gt;A. okora&lt;/i&gt; &lt;b&gt;n. sp.&lt;/b&gt;, with a value of 0.041. The intra-populational distance ranged from 0.000 in &lt;i&gt;A. parva&lt;/i&gt; to 0.002 in &lt;i&gt;A. okora&lt;/i&gt; &lt;b&gt;n. sp.&lt;/b&gt; However, &lt;i&gt;A. parana and A. schmitti&lt;/i&gt; showed an intraspecific variation of up to 0.044 and 0.024, respectively (Tab. 2).&lt;/p&gt; &lt;p&gt; The GMYC analysis suggests the presence of seven independent strains within the analyzed samples. All sequences of &lt;i&gt;Aegla okora&lt;/i&gt; &lt;b&gt;n. sp.&lt;/b&gt; were grouped in a single clade, indicating a single species. On the other hand, the disjunct distribution of the sequences of two species analyzed (&lt;i&gt;A. parana and A. schmitti&lt;/i&gt;) suggests merophyletic clusters (Fig. 7).&lt;/p&gt;Published as part of &lt;i&gt;Páez, Fernanda Polli, Marçal, Ingrid Costa, Souza-Shibatta, Lenice, Gregati, Rafael Augusto, Sofia, Silvia Helena &amp; Teixeira, Gustavo Monteiro, 2018, A new species of Aegla Leach, 1820 (Crustacea, Anomura) from the Iguaçu River basin, Brazil, pp. 335-346 in Zootaxa 4527 (3)&lt;/i&gt; on pages 338-342, DOI: 10.11646/zootaxa.4527.3.3, &lt;a href="http://zenodo.org/record/2612284"&gt;http://zenodo.org/record/2612284&lt;/a&gt

Decapoda Latreille 1802

Decapoda Latreille, 1802 Anomura H. Milne Edwards, 1832Published as part of &lt;i&gt;Páez, Fernanda Polli, Marçal, Ingrid Costa, Souza-Shibatta, Lenice, Gregati, Rafael Augusto, Sofia, Silvia Helena &amp; Teixeira, Gustavo Monteiro, 2018, A new species of Aegla Leach, 1820 (Crustacea, Anomura) from the Iguaçu River basin, Brazil, pp. 335-346 in Zootaxa 4527 (3)&lt;/i&gt; on page 338, DOI: 10.11646/zootaxa.4527.3.3, &lt;a href="http://zenodo.org/record/2612284"&gt;http://zenodo.org/record/2612284&lt;/a&gt

FIGURE 5. Aegla okora n in A new species of Aegla Leach, 1820 (Crustacea, Anomura) from the Iguaçu River basin, Brazil

FIGURE 5. Aegla okora n. sp., male holotype, CLE 21.3 mm (MZUEL 250). A, Dorsal view of dactylus and carpus of major cheliped (left). B, Dorsal view of dactylus, propodus and carpus of minor cheliped (right). Note: A and B, palmar crest (arrow)

FIGURE 7 in A new species of Aegla Leach, 1820 (Crustacea, Anomura) from the Iguaçu River basin, Brazil

FIGURE 7. The Bayesian inference tree for Aegla species, obtained using the COI gene in BEAST v.2.2.1. The numbers above the branches are the posterior probability values (values <50% are not indicated). The vertical line indicates the species delimitation assigned by the single-threshold method in GMYC analysis

A new species of Aegla Leach, 1820 (Crustacea, Anomura) from the Iguaçu River basin, Brazil

Páez, Fernanda Polli, Marçal, Ingrid Costa, Souza-Shibatta, Lenice, Gregati, Rafael Augusto, Sofia, Silvia Helena, Teixeira, Gustavo Monteiro (2018): A new species of Aegla Leach, 1820 (Crustacea, Anomura) from the Iguaçu River basin, Brazil. Zootaxa 4527 (3): 335-346, DOI: https://doi.org/10.11646/zootaxa.4527.3.