EVALUATION OF INTRA-MUSCLAR OXYGENATION DURING EXERCISE IN HUMANS

Near infrared spectroscopy (NIRS) has been frequently used to assess intra-muscular oxygenation past few decades. In recent years, refinement of NIRS hardware and algorithms used to convert changes in optical absorption to changes in concentration improved the validity of oxygenated haemoglobin (HbO2), deoxyhaemoglobin (Hb), myoglobin (Mb) and the oxidised copper compound of cytochrome aa3, (cyt aa3) measurements. Subsequently, the use of NIRS to study the muscle oxygenation profiles during various types of exercise and to monitor differences in oxygenation levels in patients under various pathological conditions is being increased. It would be thought-provoking to combine future muscle studies with MRS and/or electromyography techniques to improve the understanding of intramuscular oxygenation

EFFECT OF TIME OF DAY ON THE RELATIONSHIP BETWEEN LACTATE AND VENTILATORY THRESHOLDS: A BRIEF REPORT

The purpose of this investigation was to study the effect of time of day on the relationship between lactate (LT) and ventilatory thresholds (VT) of pulmonary oxygen uptake (VO2). Seven moderately active male volunteers (26.3±3.0 years, 1.74±0.08 m, 76±5 kg) performed a maximal incremental test (increases of 30 W every 2 min) on a cycle ergometer on consecutive days at 0900 h, 1400h and 1900 h in a randomized fashion. The anaerobic threshold was determined using both ventilatory gas analysis and blood lactate measures. Each of the following variables was recorded both at VT and the LT; heart rate (HR, beats.min-1), minute ventilation (VE, L.min-1), respiratory exchange ratio (RER), time to threshold (Time, sec), oxygen uptake (VO2, ml.kg-1.min-1) and VO2 as a percentage of maximal oxygen uptake (%VO2max). The correlations between VT and LT variables analyzed by Pearson product moment correlations for each time of day. ANOVA was used to compare the data obtained at different times of the day. There were no significant differences for the data related to time of day either for ventilatory gas analysis or lactate measurements. The correlation coefficients between VT and LT variables were moderate to high (r=0.56-0.94) for time of day. However, the correlations for HR, VO2, and %VO2max (r=0.81-0.94) were slightly stronger compared with Time, VE and RER (r=0.56-0.88). It was concluded that, the data at VT and LT were not influenced by time of da

Irisin relaxes rat thoracic aorta: MEK1/2 signaling pathway, KV channels, SKCa channels, and BKCa channels are involved in irisin-induced vasodilation

In this study, it was aimed to investigate the effects of irisin on vascular smooth muscle contractility in rat thoracic aorta, and the hypothesis that mitogen-activated protein kinase kinase (MEK1/2) signalling pathway, voltage-gated potassium (KV) channels, small-conductance calcium-activated potassium (SKCa) channels, and large-conductance calcium-activated potassium (BKCa) channels may have roles in these effects. Isometric contraction-relaxation responses of isolated thoracic aorta rings were measured with an organ bath model. The steady contraction was induced with 10-5 M phenylephrine (PHE), and then the concentration-dependent responses of irisin (10-9-10-6 M) were examined. Irisin exerted the vasorelaxant effects at concentrations of 10-8, 10-7, and 10-6 M compared to the control group (pThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author